anti-Horse (Equine) IL1B antibody for Western Blotting

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL1B
Epitope
N-Term
38
29
29
15
15
15
15
14
13
12
8
8
8
7
7
7
6
6
6
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Horse (Equine), Human, Mouse (Murine), Rabbit
510
189
139
37
27
21
19
14
14
11
10
9
8
4
4
2
2
1
1
Host
Rabbit
433
331
17
10
10
7
6
2
2
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
102
45
32
15
14
11
10
9
9
8
8
8
8
8
8
8
5
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
547
379
215
133
102
74
62
56
44
40
33
32
23
16
14
5
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Options
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Pubmed
2 references available

Available images

Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN5326748)
Immunohistochemistry (IHC) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN5326748)
Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN5326748)
Catalog No. ABIN5326748
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1357021 ABIN1173150 ICC IHC WB Rabbit IgG AA 63-247 Log in to see Polyclonal 0
3.1357021 ABIN3201450 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 63-247 Log in to see 0
3.1357021 ABIN2935277 IF/ICC IHC IP WB Rabbit AA 63-247 Log in to see Polyclonal 0
3.1357021 ABIN6002459 IF/ICC IHC IP WB Mouse Log in to see 0
1 ABIN575295 WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN2609655 WB Mouse IgG AA 63-247 Log in to see 0
1 ABIN2609633 WB Rabbit IgG AA 63-247 Log in to see Polyclonal 0
1 ABIN2609642 WB Biotin Rabbit IgG AA 63-247 Log in to see Polyclonal 0
1 ABIN2609646 WB FITC Rabbit IgG AA 63-247 Log in to see Polyclonal 0
1 ABIN2474895 IHC (fro) FACS ELISA WB Mouse IgG1 Log in to see 1D4 4
1 ABIN6290527 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2298096 ELISA WB Rat IgG2a AA 116-268 Log in to see 8B25 0
1 ABIN1173152 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN6050367 IF/ICC IHC WB Biotin Rabbit IgG Log in to see Polyclonal 0

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope N-Term
(38), (29), (29), (15), (15), (15), (15), (14), (13), (12), (8), (8), (8), (7), (7), (7), (6), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Horse (Equine), Human, Mouse (Murine), Rabbit
(510), (189), (139), (37), (27), (21), (19), (14), (14), (11), (10), (9), (8), (4), (4), (2), (2), (1), (1)
Host Rabbit
(433), (331), (17), (10), (10), (7), (6), (2), (2)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(102), (45), (32), (15), (14), (11), (10), (9), (9), (8), (8), (8), (8), (8), (8), (8), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(547), (379), (215), (133), (102), (74), (62), (56), (44), (40), (33), (32), (23), (16), (14), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B antibody (ABIN5326748) Images
Blocking Peptide Blocking peptide for this product available: ABIN978485
Sequence MAEVPELASE MMAYYSGNED DLFFEADGPK QMKCSFQDLD LCPLDGGIQL
Predicted Reactivity Dog: 92%, Horse: 86%, Human: 100%, Mouse: 75%, Rabbit: 92%
Characteristics This is a rabbit polyclonal antibody against IL1B. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human IL1B
Plasmids, Primers & others

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B antibody (ABIN5326748) Images back to top
Antigen
Alternative Name IL1B (IL1B Antibody Abstract)
Background IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. The gene encoding IL1B and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
Alias Symbols: IL-1, IL1-BETA, IL1F2
Protein Interaction Partner: PTPN1, LYN, FYN, CMA1, CASP1, APP, SP1, EGR1, ELAVL1, CASP4, IL1RAP, PRTN3, IL1R2, IL1R1, IL1B, MMP2, ADRB2, A2M, UBE2N, ZNF675, MAPK8IP2,
Protein Size: 269
Molecular Weight 17 kDa
Gene ID 3553
NCBI Accession NM_000576, NP_000567
UniProt P01584
Pathways NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B antibody (ABIN5326748) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 269 AA
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B antibody (ABIN5326748) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-IL1B antibody (ABIN5326748)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Product cited in:

Lee, Lu, Chai, Sheu, Chen, Huang, Kao, Chen, Chung, Sung, Chang, Lee, Yip: "Circulating microparticles enhanced rat vascular wall remodeling following endothelial denudation." in: American journal of translational research, Vol. 8, Issue 11, pp. 4511-4522, 2016

Wang, Jiang, Li, He, Zhao, Yang, Zhu, Yin, Li, Wang, Liu, Shang, Yang, Wang, Zhen, Zhang, Yu, Zhong, Ye, Huang, Yuan: "Shigella flexneri T3SS effector IpaH4.5 modulates the host inflammatory response via interaction with NF-?B p65 protein." in: Cellular microbiology, Vol. 15, Issue 3, pp. 474-85, 2013

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B antibody (ABIN5326748) back to top
Supplier Images
Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN5326748) WB Suggested Anti-IL1B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Immunohistochemistry (IHC) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN5326748) Sample Type: Human Macrophange Cells
Green: primary
Red: p...
Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN5326748) WB Suggested Anti-IL1B
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:12500