anti-Horse (Equine) IL1B antibody for Western Blotting
Recommended IL1B Antibody (supplied by: Log in to see )
- Antigen
-
Interleukin 1, beta (IL1B) Antibodies
show synonyms for this antigen- il1-b
- zgc:111873
- IL-1beta
- Il-1b
- IL1B
- IL-1
- IL1-BETA
- IL1F2
- IL-1BETA
- IL1beta
- IL-1B
- interleukin 1, beta
- interleukin 1 beta
- il1b
- Il1b
- IL1B
- Epitope
-
N-Term
Alternatives - Reactivity
-
Dog (Canine), Horse (Equine), Human, Mouse (Murine), Rabbit
Alternatives - Host
-
Rabbit
Alternatives - Clonality
- Conjugate
-
This IL1B antibody is un-conjugated
Alternatives - Application
-
Immunohistochemistry (IHC), Western Blotting (WB)
Alternatives - Options
- Supplier
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- Supplier Product No.
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Catalog No. ABIN5326748
$ 289.00
Plus shipping costs $45.00
Plus shipping costs $45.00
| Relevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
|---|---|---|---|---|---|---|---|---|---|---|
| 3.0962598 | ABIN1173150 | ICC IHC WB | Rabbit | IgG | AA 63-247 | Log in to see | Polyclonal | 0 | ||
| 3.0962598 | ABIN3201450 | ICC IHC (fro) IHC (p) ELISA WB | Mouse | IgG | AA 63-247 | Log in to see | 0 | |||
| 3.0962598 | ABIN2935277 | IF/ICC IHC IP WB | Rabbit | AA 63-247 | Log in to see | Polyclonal | 0 | |||
| 3.0962598 | ABIN6002459 | IF/ICC IHC IP WB | Mouse | Log in to see | 0 | |||||
| 1 | ABIN575295 | WB | Rabbit | N-Term | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN2609655 | WB | Mouse | IgG | AA 63-247 | Log in to see | 0 | |||
| 1 | ABIN2609633 | WB | Rabbit | IgG | AA 63-247 | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN2609642 | WB | Biotin | Rabbit | IgG | AA 63-247 | Log in to see | Polyclonal | 0 | |
| 1 | ABIN2609646 | WB | FITC | Rabbit | IgG | AA 63-247 | Log in to see | Polyclonal | 0 | |
| 1 | ABIN2474895 | IHC (fro) FACS ELISA WB | Mouse | IgG1 | Log in to see | 1D4 | 4 | |||
| 1 | ABIN2298096 | ELISA WB | Rat | IgG2a | AA 116-268 | Log in to see | 8B25 | 0 | ||
| 1 | ABIN1173152 | IHC ELISA WB | Biotin | Rabbit | IgG | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN6050367 | IF/ICC IHC WB | Biotin | Rabbit | IgG | Log in to see | Polyclonal | 0 |
General |
|
|---|---|
| Antigen | Interleukin 1, beta (IL1B) Antibodies |
| Epitope | N-Term Alternatives |
| Reactivity | Dog (Canine), Horse (Equine), Human, Mouse (Murine), Rabbit Alternatives |
| Host | Rabbit Alternatives |
| Clonality | |
| Conjugate | This IL1B antibody is un-conjugated Alternatives |
| Application |
Immunohistochemistry (IHC), Western Blotting (WB)
Alternatives
|
|
2 references available |
| Supplier | Log in to see |
Product Details anti-IL1B AntibodyTarget Details IL1B Application Details Handling References for anti-IL1B antibody (ABIN5326748) Images |
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| Blocking Peptide | Blocking peptide for this product available: ABIN978485 |
| Sequence | MAEVPELASE MMAYYSGNED DLFFEADGPK QMKCSFQDLD LCPLDGGIQL |
| Predicted Reactivity | Dog: 92%, Horse: 86%, Human: 100%, Mouse: 75%, Rabbit: 92% |
| Characteristics | This is a rabbit polyclonal antibody against IL1B. It was validated on Western Blot using a cell lysate as a positive control. |
| Purification | Affinity Purified |
| Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human IL1B |
| Plasmids, Primers & others | |
Target Details IL1BProduct Details anti-IL1B Antibody Application Details Handling References for anti-IL1B antibody (ABIN5326748) Images back to top |
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| Antigen | |
| Alternative Name | IL1B (IL1B Antibody Abstract) |
| Background |
IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. The gene encoding IL1B and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Alias Symbols: IL-1, IL1-BETA, IL1F2 Protein Interaction Partner: PTPN1, LYN, FYN, CMA1, CASP1, APP, SP1, EGR1, ELAVL1, CASP4, IL1RAP, PRTN3, IL1R2, IL1R1, IL1B, MMP2, ADRB2, A2M, UBE2N, ZNF675, MAPK8IP2, Protein Size: 269 |
| Molecular Weight | 17 kDa |
| Gene ID | 3553 |
| NCBI Accession | NM_000576, NP_000567 |
| UniProt | P01584 |
| Pathways | NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy |
Application DetailsProduct Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B antibody (ABIN5326748) Images back to top |
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| Application Notes | Optimal working dilutions should be determined experimentally by the investigator. |
| Comment |
Antigen size: 269 AA |
| Restrictions | For Research Use only |
HandlingProduct Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B antibody (ABIN5326748) Images back to top |
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| Format | Liquid |
| Concentration | Lot specific |
| Buffer | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose. |
| Preservative | Sodium azide |
| Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Handling Advice | Avoid repeated freeze-thaw cycles. |
| Storage | -20 °C |
| Storage Comment | For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
References for anti-IL1B antibody (ABIN5326748)Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top |
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| Product cited in: |
Lee, Lu, Chai, Sheu, Chen, Huang, Kao, Chen, Chung, Sung, Chang, Lee, Yip: "Circulating microparticles enhanced rat vascular wall remodeling following endothelial denudation." in: American journal of translational research, Vol. 8, Issue 11, pp. 4511-4522, 2016 Wang, Jiang, Li, He, Zhao, Yang, Zhu, Yin, Li, Wang, Liu, Shang, Yang, Wang, Zhen, Zhang, Yu, Zhong, Ye, Huang, Yuan: "Shigella flexneri T3SS effector IpaH4.5 modulates the host inflammatory response via interaction with NF-?B p65 protein." in: Cellular microbiology, Vol. 15, Issue 3, pp. 474-85, 2013 |
ImagesProduct Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B antibody (ABIN5326748) back to top |
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| Supplier Images |
WB Suggested Anti-IL1B Antibody Titration: 0.2-1 ug/mlELISA Titer: ... 
Sample Type: Human Macrophange CellsGreen: primary Red: p... 
WB Suggested Anti-IL1B Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:12500 |
