anti-Human IL1B antibody for Western Blotting

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL1B
Reactivity
Human
510
192
140
37
28
21
19
15
15
11
10
9
8
4
4
2
2
1
1
Host
Mouse
439
324
19
10
9
8
6
2
2
Clonality
Monoclonal
Conjugate
This IL1B antibody is un-conjugated
102
45
32
14
14
11
10
9
9
8
8
8
8
8
8
8
5
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
553
380
215
132
97
74
63
52
44
40
36
31
23
16
14
5
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN969215
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
27.251972 ABIN4899223 CyTOF FACS ICC Neut WB Mouse IgG1 AA 117-269 Log in to see 8516 15
26.5 ABIN728503 IF (p) IHC (p) WB Rabbit IgG AA 120-170 Log in to see Polyclonal 11
23.5 ABIN872644 IF (p) IHC (p) WB Rabbit IgG AA 190-240 Log in to see Polyclonal 2
22.563305 ABIN3187737 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal 0
22.563305 ABIN103012 FACS IP IHC Neut RIA ELISA WB Rabbit IgG Cleavage Site, N-Term, AA 153 Log in to see Polyclonal 0
21.251972 ABIN516996 IF IP ELISA WB Mouse IgG2b kappa AA 170-269 Log in to see 2A8 1
19.751972 ABIN6262623 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 9
19.751972 ABIN4880333 ELISA ICC IHC WB Mouse IgG1 Log in to see 3A6 0
19.751972 ABIN1845349 ELISA IF/ICC IHC WB Mouse IgG1 Log in to see 0
19.751972 ABIN1107772 EIA IF IHC (p) WB Mouse IgG1 Log in to see 3A6 2
19.563305 ABIN3185165 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
19.493721 ABIN269770 ELISA EM ICC IF IHC IHC (p) IP WB Rabbit N-Term Log in to see Polyclonal 16
19 ABIN1498871 ELISA IF IHC IHC (p) WB Mouse IgG1 Log in to see 3A6 0
18.251972 ABIN652470 FACS IHC (p) WB Rabbit Ig Fraction AA 148-174, Center Log in to see Polyclonal 5
17.311333 ABIN966384 WB Rabbit IgG C-Term Log in to see Polyclonal 1
16.751972 ABIN395309 ELISA IF IP WB Mouse IgG2b kappa AA 170-269 Log in to see 2A8 5
16.751972 ABIN5580782 ELISA IF IHC (p) WB Mouse IgG1 Log in to see 3A6 0
16.563305 ABIN964768 FACS IP IHC Neut RIA ELISA WB HRP Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal 0
16.563305 ABIN964752 FACS IP IHC Neut RIA ELISA WB Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal 0
16.563305 ABIN634267 WB Rabbit N-Term Log in to see Polyclonal 0

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Human
(510), (192), (140), (37), (28), (21), (19), (15), (15), (11), (10), (9), (8), (4), (4), (2), (2), (1), (1)
Host Mouse
(439), (324), (19), (10), (9), (8), (6), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL1B antibody is un-conjugated
(102), (45), (32), (14), (14), (11), (10), (9), (9), (8), (8), (8), (8), (8), (8), (8), (5), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(553), (380), (215), (132), (97), (74), (63), (52), (44), (40), (36), (31), (23), (16), (14), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B antibody (ABIN969215) Images
Purification Ascites
Immunogen Purified recombinant fragment of human IL1B expressed in E. Coli.
Clone 3A6
Isotype IgG1
Plasmids, Primers & others

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B antibody (ABIN969215) Images back to top
Antigen
Alternative Name IL1B (IL1B Antibody Abstract)
Background The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
Molecular Weight 31 kDa
Gene ID 3553
HGNC 3553
Pathways NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B antibody (ABIN969215) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B antibody (ABIN969215) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-IL1B antibody (ABIN969215)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Product cited in:

Mohideen, Capili, Bilimoria, Yamada, Bonni, Lima: "A molecular basis for phosphorylation-dependent SUMO conjugation by the E2 UBC9." in: Nature structural & molecular biology, Vol. 16, Issue 9, pp. 945-52, 2009

Kuo, Bentsi-Barnes, Barlow, Bae, Pisarska: "Sumoylation of forkhead L2 by Ubc9 is required for its activity as a transcriptional repressor of the Steroidogenic Acute Regulatory gene." in: Cellular signalling, Vol. 21, Issue 12, pp. 1935-44, 2009

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B antibody (ABIN969215) back to top
Supplier Images
ELISA image for anti-Interleukin 1, beta (IL1B) antibody (ABIN969215) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Immunocytochemistry (ICC) image for anti-Interleukin 1, beta (IL1B) antibody (ABIN969215) Figure 2: Immunofluorescence analysis of HepG2 cells using IL1B mouse mAb (green) . B...
Immunohistochemistry (IHC) image for anti-Interleukin 1, beta (IL1B) antibody (ABIN969215) Figure 3: Immunohistochemical analysis of paraffin-embedded muscle tissues using IL1B...
Immunohistochemistry (IHC) image for anti-Interleukin 1, beta (IL1B) antibody (ABIN969215) Figure 4: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using...
Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) antibody (ABIN969215) Figure 5: Western blot analysis using IL1B mAb against human IL1B (AA: 126-261) recom...
Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) antibody (ABIN969215) Figure 6: Western blot analysis using IL1B mAb against HEK293 (1) and IL1B-hIgGFc tra...