anti-Mouse (Murine) IL1B antibody for Immunocytochemistry

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL1B
Epitope
N-Term
42
31
30
20
16
15
15
14
12
11
11
7
7
7
6
6
6
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Bacteria, Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus)
538
189
127
38
28
20
16
13
12
12
9
7
6
4
4
2
2
2
1
Host
Rabbit
420
353
16
16
8
7
6
2
2
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
97
46
33
24
18
15
13
12
10
10
8
8
8
8
8
8
8
4
4
4
4
4
4
2
2
2
1
1
1
1
1
1
1
1
Application
ELISA, Electron Microscopy (EM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
526
419
175
150
112
74
59
42
42
32
29
24
16
14
14
7
5
4
4
3
3
3
3
3
2
1
1
1
1
1
1
Supplier
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Pubmed
11 references available

Available images

Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN269770)
Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN269770)
Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN269770)
Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN269770)
Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN269770)
Immunohistochemistry (IHC) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN269770)
Catalog No. ABIN269770
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4324365 ELISA FACS ICC IF IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 4
1 ABIN1859383 ICC IHC WB Rabbit IgG AA 118-269 Log in to see Polyclonal 0
1 ABIN1173157 ICC IHC WB Rabbit IgG AA 118-269 Log in to see Polyclonal 0
1 ABIN5565692 FACS ICC IF IHC APC Rabbit AA 118-269 Log in to see Polyclonal 0
1 ABIN5565694 FACS ICC IF IHC FITC Rabbit AA 118-269 Log in to see Polyclonal 0
1 ABIN5565696 FACS ICC IF IHC PE Rabbit AA 118-269 Log in to see Polyclonal 0
1 ABIN1982628 ICC IHC WB Sheep IgG AA 118-269 Log in to see Polyclonal 0
1 ABIN1980439 ICC IHC (fro) IHC (p) ELISA WB Mouse AA 118-269 Log in to see 0

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope N-Term
(42), (31), (30), (20), (16), (15), (15), (14), (12), (11), (11), (7), (7), (7), (6), (6), (6), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bacteria, Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus)
(538), (189), (127), (38), (28), (20), (16), (13), (12), (12), (9), (7), (6), (4), (4), (2), (2), (2), (1)
Host Rabbit
(420), (353), (16), (16), (8), (7), (6), (2), (2)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(97), (46), (33), (24), (18), (15), (13), (12), (10), (10), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Electron Microscopy (EM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(526), (419), (175), (150), (112), (74), (59), (42), (42), (32), (29), (24), (16), (14), (14), (7), (5), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 11 references available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B antibody (ABIN269770) Images
Specificity Detects IL-1 beta and does not cross react with IL-1 alpha. Weakly detects the non-denatured 31 kDa precursor molecule but strongly detects all of the 17 kDa mature molecule. Additionally this IL-1 beta antibody will bind the denatured 31 kDa precursor molecule with greater affinity than the native form, but will often detect past the 35 kDa mark. This IL-1 beta antibody has also been shown to be useful in neutralization of human and primate IL-1 beta activity in bioassays.
Purification IgG purified
Immunogen Recombinant 153 aa human IL-1beta produced in E.coli. MW of recombinant IL-1beta 15kDa, with the N-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1beta converting enzyme (ICE, capase-1).

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B antibody (ABIN269770) Images back to top
Antigen
Alternative Name IL-1 beta/il-1F2 (IL1B Antibody Abstract)
Background Gene Symbol: IL1B
Gene ID 3553
UniProt P01584
Pathways NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B antibody (ABIN269770) Images back to top
Application Notes Western Blot 1:1000, ELISA 1:500-1:2000, Immunohistochemistry 1:100-1:200, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:400-1:800, Immunohistochemistry-Paraffin 1:10-1:500, Electron MicroscopyAnti-Human IL-1B has been tested for use in neutralizations, ELISA, radioimmunoassays, flow cytometry, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17,000 MW mature IL-1B. For immunoblots, typically, IL-1B is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) serum plus 10 ng/mL E.coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG (p/n 011-001-297) to reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL-1B. For ELISA use HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (611-1302) for detection. In ELISA formats this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This antibody is also useful for neutralization of human and primate IL-1B activity in bioassays. It does not neutralize the biological activity IL-1a. It does not neutralize the biological activity of murine, rat or rabbit IL-1B. For neutralization, it is recommended to incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F(c) domain of the rabbit IgG molecule may interact with cells non-specifically.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B antibody (ABIN269770) Images back to top
Format Liquid
Concentration 2.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-IL1B antibody (ABIN269770)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Product cited in:

Ahmad, Chung, Tang, Hockman, Liu, Khan, Huo, Billings, Amar, Remaley, Manganiello: "Phosphodiesterase 3B (PDE3B) regulates NLRP3 inflammasome in adipose tissue." in: Scientific reports, Vol. 6, pp. 28056, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Rossi, Cadamuro, Biselli-Périco, Leite, Severino, Reis, Cordeiro, Silva: "Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection." in: Cellular microbiology, Vol. 18, Issue 10, pp. 1444-58, 2016 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Ahlstrand, Tuominen, Beklen, Torittu, Oscarsson, Sormunen, Pöllänen, Permi, Ihalin: "A novel intrinsically disordered outer membrane lipoprotein of Aggregatibacter actinomycetemcomitans binds various cytokines and plays a role in biofilm response to interleukin-1β and interleukin-8." in: Virulence, Vol. 8, Issue 2, pp. 115-134, 2016

Su, Luo, Zhu, Fu, Zhao, Chen, Zhang, Ren, Yu, Yang, Wu, Feng, Li, Chen, Wang: "Interleukin-1β/Iinterleukin-1 receptor-associated kinase 1 inflammatory signaling contributes to persistent Gankyrin activation during hepatocarcinogenesis." in: Hepatology (Baltimore, Md.), Vol. 61, Issue 2, pp. 585-97, 2015 Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Bimpis, Papalois, Voumvourakis, Oláh, Tiszlavicz, Liapi: "Neuronal tumour necrosis factor-α and interleukin-1β expression in a porcine model of intracerebral haemorrhage: Modulation by U-74389G." in: Brain research, Vol. 1615, pp. 98-105, 2015 (Sample species: Pig (Porcine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Arendt, McCready, Keller, Baker, Naber, Seewaldt, Kuperwasser: "Obesity promotes breast cancer by CCL2-mediated macrophage recruitment and angiogenesis." in: Cancer research, Vol. 73, Issue 19, pp. 6080-93, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunoprecipitation

Paino, Ahlstrand, Nuutila, Navickaite, Lahti, Tuominen, Välimaa, Lamminmäki, Pöllänen, Ihalin: "Identification of a novel bacterial outer membrane interleukin-1Β-binding protein from Aggregatibacter actinomycetemcomitans." in: PLoS ONE, Vol. 8, Issue 7, pp. e70509, 2013 (Sample species: Bacteria). Further details: Western Blotting

Paino, Lohermaa, Sormunen, Tuominen, Korhonen, Pöllänen, Ihalin: "Interleukin-1β is internalised by viable Aggregatibacter actinomycetemcomitans biofilm and locates to the outer edges of nucleoids." in: Cytokine, Vol. 60, Issue 2, pp. 565-74, 2012 (Sample species: Bacteria). Further details: Immunoelectron Microscopy,Immunohistochemistry

Petrovski, Ayna, Majai, Hodrea, Benko, Mádi, Fésüs: "Phagocytosis of cells dying through autophagy induces inflammasome activation and IL-1β release in human macrophages." in: Autophagy, Vol. 7, Issue 3, pp. 321-30, 2011 (Sample species: Human). Further details: Western Blotting

Paino, Tuominen, Jääskeläinen, Alanko, Nuutila, Asikainen, Pelliniemi, Pöllänen, Chen, Ihalin: "Trimeric form of intracellular ATP synthase subunit β of Aggregatibacter actinomycetemcomitans binds human interleukin-1β." in: PLoS ONE, Vol. 6, Issue 4, pp. e18929, 2011

Komiya, Shimizu, Noma, Tsuboi, Honda, Kanno, Ohara, Shinoda, Ogiso, Iwata: "Role of Neuron-Glial Interaction Mediated by IL-1β in Ectopic Tooth Pain." in: Journal of dental research, pp. 22034517741253, 1970 Method employed by authors: Western Blotting (WB)

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B antibody (ABIN269770) back to top
Supplier Images
Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN269770) anti-Interleukin 1, beta (IL1B) (N-Term) antibody
Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN269770) Western Blot: IL-1 beta/IL-1F2 Antibody [ABIN269770] - Lane 1: Human IL-1Beta. Load: ...
Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN269770) Western Blot: IL-1 beta/IL-1F2 Antibody [ABIN269770] - Antibody was used at a 1:200 d...
Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN269770) Western Blot: IL-1 beta/IL-1F2 Antibody [ABIN269770] - pro-IL-1beta in glioblastoma c...
Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN269770) Western Blot: IL-1 beta/IL-1F2 Antibody [ABIN269770] - Analysis of IL-1 beta in Rhesu...
Immunohistochemistry (IHC) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN269770) Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [ABIN269770] - Analysis of: Human IL1...
Immunofluorescence (IF) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN269770) Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody [ABIN269770] - Trab...