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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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1 | ABIN4324365 | ELISA FACS ICC IF IHC IHC (p) IP WB | Rabbit | Log in to see | Polyclonal | 4 | ||||
1 | ABIN1859383 | ICC IHC WB | Rabbit | IgG | AA 118-269 | Log in to see | Polyclonal | 0 | ||
1 | ABIN1173157 | ICC IHC WB | Rabbit | IgG | AA 118-269 | Log in to see | Polyclonal | 0 | ||
1 | ABIN5565692 | FACS ICC IF IHC | APC | Rabbit | AA 118-269 | Log in to see | Polyclonal | 0 | ||
1 | ABIN5565694 | FACS ICC IF IHC | FITC | Rabbit | AA 118-269 | Log in to see | Polyclonal | 0 | ||
1 | ABIN5565696 | FACS ICC IF IHC | PE | Rabbit | AA 118-269 | Log in to see | Polyclonal | 0 | ||
1 | ABIN1982628 | ICC IHC WB | Sheep | IgG | AA 118-269 | Log in to see | Polyclonal | 0 | ||
1 | ABIN1980439 | ICC IHC (fro) IHC (p) ELISA WB | Mouse | AA 118-269 | Log in to see | 0 |
General |
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Antigen | Interleukin 1, beta (IL1B) Antibodies |
Epitope | N-Term Alternatives |
Reactivity | Bacteria, Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus) Alternatives |
Host | Rabbit Alternatives |
Clonality | |
Conjugate | This IL1B antibody is un-conjugated Alternatives |
Application |
ELISA, Electron Microscopy (EM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Alternatives
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11 references available |
Supplier | Log in to see |
Product Details anti-IL1B AntibodyTarget Details IL1B Application Details Handling References for anti-IL1B antibody (ABIN269770) Images |
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Specificity | Detects IL-1 beta and does not cross react with IL-1 alpha. Weakly detects the non-denatured 31 kDa precursor molecule but strongly detects all of the 17 kDa mature molecule. Additionally this IL-1 beta antibody will bind the denatured 31 kDa precursor molecule with greater affinity than the native form, but will often detect past the 35 kDa mark. This IL-1 beta antibody has also been shown to be useful in neutralization of human and primate IL-1 beta activity in bioassays. |
Purification | IgG purified |
Immunogen | Recombinant 153 aa human IL-1beta produced in E.coli. MW of recombinant IL-1beta 15kDa, with the N-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1beta converting enzyme (ICE, capase-1). |
Target Details IL1BProduct Details anti-IL1B Antibody Application Details Handling References for anti-IL1B antibody (ABIN269770) Images back to top |
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Antigen | |
Alternative Name | IL-1 beta/il-1F2 (IL1B Antibody Abstract) |
Background | Gene Symbol: IL1B |
Gene ID | 3553 |
UniProt | P01584 |
Pathways | NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy |
Application DetailsProduct Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B antibody (ABIN269770) Images back to top |
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Application Notes | Western Blot 1:1000, ELISA 1:500-1:2000, Immunohistochemistry 1:100-1:200, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:400-1:800, Immunohistochemistry-Paraffin 1:10-1:500, Electron MicroscopyAnti-Human IL-1B has been tested for use in neutralizations, ELISA, radioimmunoassays, flow cytometry, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17,000 MW mature IL-1B. For immunoblots, typically, IL-1B is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) serum plus 10 ng/mL E.coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG (p/n 011-001-297) to reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL-1B. For ELISA use HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (611-1302) for detection. In ELISA formats this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This antibody is also useful for neutralization of human and primate IL-1B activity in bioassays. It does not neutralize the biological activity IL-1a. It does not neutralize the biological activity of murine, rat or rabbit IL-1B. For neutralization, it is recommended to incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F(c) domain of the rabbit IgG molecule may interact with cells non-specifically. |
Comment |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
Restrictions | For Research Use only |
HandlingProduct Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B antibody (ABIN269770) Images back to top |
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Format | Liquid |
Concentration | 2.0 mg/mL |
Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Buffer contains: No Preservative |
Preservative | Without preservative |
Handling Advice | Avoid freeze-thaw cycles |
Storage | -80 °C,-20 °C |
Storage Comment | Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles. |
References for anti-IL1B antibody (ABIN269770)Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top |
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Product cited in: |
Ahmad, Chung, Tang, Hockman, Liu, Khan, Huo, Billings, Amar, Remaley, Manganiello: "Phosphodiesterase 3B (PDE3B) regulates NLRP3 inflammasome in adipose tissue." in: Scientific reports, Vol. 6, pp. 28056, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting Rossi, Cadamuro, Biselli-Périco, Leite, Severino, Reis, Cordeiro, Silva: "Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection." in: Cellular microbiology, Vol. 18, Issue 10, pp. 1444-58, 2016 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections) Ahlstrand, Tuominen, Beklen, Torittu, Oscarsson, Sormunen, Pöllänen, Permi, Ihalin: "A novel intrinsically disordered outer membrane lipoprotein of Aggregatibacter actinomycetemcomitans binds various cytokines and plays a role in biofilm response to interleukin-1β and interleukin-8." in: Virulence, Vol. 8, Issue 2, pp. 115-134, 2016 Su, Luo, Zhu, Fu, Zhao, Chen, Zhang, Ren, Yu, Yang, Wu, Feng, Li, Chen, Wang: "Interleukin-1β/Iinterleukin-1 receptor-associated kinase 1 inflammatory signaling contributes to persistent Gankyrin activation during hepatocarcinogenesis." in: Hepatology (Baltimore, Md.), Vol. 61, Issue 2, pp. 585-97, 2015 Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections) Bimpis, Papalois, Voumvourakis, Oláh, Tiszlavicz, Liapi: "Neuronal tumour necrosis factor-α and interleukin-1β expression in a porcine model of intracerebral haemorrhage: Modulation by U-74389G." in: Brain research, Vol. 1615, pp. 98-105, 2015 (Sample species: Pig (Porcine)). Further details: Immunohistochemistry (Paraffin-embedded Sections) Arendt, McCready, Keller, Baker, Naber, Seewaldt, Kuperwasser: "Obesity promotes breast cancer by CCL2-mediated macrophage recruitment and angiogenesis." in: Cancer research, Vol. 73, Issue 19, pp. 6080-93, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunoprecipitation Paino, Ahlstrand, Nuutila, Navickaite, Lahti, Tuominen, Välimaa, Lamminmäki, Pöllänen, Ihalin: "Identification of a novel bacterial outer membrane interleukin-1Β-binding protein from Aggregatibacter actinomycetemcomitans." in: PLoS ONE, Vol. 8, Issue 7, pp. e70509, 2013 (Sample species: Bacteria). Further details: Western Blotting Paino, Lohermaa, Sormunen, Tuominen, Korhonen, Pöllänen, Ihalin: "Interleukin-1β is internalised by viable Aggregatibacter actinomycetemcomitans biofilm and locates to the outer edges of nucleoids." in: Cytokine, Vol. 60, Issue 2, pp. 565-74, 2012 (Sample species: Bacteria). Further details: Immunoelectron Microscopy,Immunohistochemistry Petrovski, Ayna, Majai, Hodrea, Benko, Mádi, Fésüs: "Phagocytosis of cells dying through autophagy induces inflammasome activation and IL-1β release in human macrophages." in: Autophagy, Vol. 7, Issue 3, pp. 321-30, 2011 (Sample species: Human). Further details: Western Blotting Paino, Tuominen, Jääskeläinen, Alanko, Nuutila, Asikainen, Pelliniemi, Pöllänen, Chen, Ihalin: "Trimeric form of intracellular ATP synthase subunit β of Aggregatibacter actinomycetemcomitans binds human interleukin-1β." in: PLoS ONE, Vol. 6, Issue 4, pp. e18929, 2011 Komiya, Shimizu, Noma, Tsuboi, Honda, Kanno, Ohara, Shinoda, Ogiso, Iwata: "Role of Neuron-Glial Interaction Mediated by IL-1β in Ectopic Tooth Pain." in: Journal of dental research, pp. 22034517741253, 1970 Method employed by authors: Western Blotting (WB) |
ImagesProduct Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B antibody (ABIN269770) back to top |
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