anti-Primate IL1B antibody for Immunohistochemistry (Frozen Sections)

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL1B
Epitope
N-Term
45
32
29
18
15
15
15
13
12
12
8
8
8
7
7
7
6
6
6
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Primate
550
194
139
38
29
21
19
14
14
12
11
9
8
4
4
2
2
2
1
1
Host
Rabbit
437
370
17
11
10
7
6
2
2
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
104
47
33
22
18
15
12
12
10
10
8
8
8
8
8
8
8
4
4
4
4
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
566
400
221
142
114
73
66
62
44
44
37
32
23
15
14
5
5
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN799612
$ 1,688.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN799831 ELISA IHC IHC (fro) IHC (p) IP WB Rabbit N-Term Log in to see Polyclonal 0

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope N-Term
(45), (32), (29), (18), (15), (15), (15), (13), (12), (12), (8), (8), (8), (7), (7), (7), (6), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Primate
(550), (194), (139), (38), (29), (21), (19), (14), (14), (12), (11), (9), (8), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(437), (370), (17), (11), (10), (7), (6), (2), (2)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(104), (47), (33), (22), (18), (15), (12), (12), (10), (10), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
(566), (400), (221), (142), (114), (73), (66), (62), (44), (44), (37), (32), (23), (15), (14), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling Images
Specificity Recombinant 153 aa human IL-1b produced in E. coli. The MW of the recombinant IL-1b was 17000 with the n-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1b converting enzyme (ICE, caspase-1).
Purification Purified IgG
Immunogen Recombinant human IL1B / IL1 Beta.

Type of Immunogen: Recombinant protein
Plasmids, Primers & others

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL1B (IL1B Antibody Abstract)
Background Name/Gene ID: IL1B
Family: IL-1

Synonyms: IL1B, IL-1B, IL-1 beta, IL1-BETA, Preinterleukin 1 beta, Pro-interleukin-1-beta, Catabolin, IL-1, IL1F2, Interleukin 1, beta, Interleukin-1 beta
Gene ID 3553
UniProt P01584
Pathways NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Application Notes Approved: ELISA (1:500 - 1:2000), IHC, IHC-Fr (1:100 - 1:200), IHC-P (1:100 - 1:200), IP (1:400 - 1:800), RIA (1:5000 - 1:10000), WB (1:100 - 1:200)

Usage: This IgG fraction antibody of anti- Human IL-1beta has been tested for use in neutralizations, ELISA, radioimmunoassays, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17000 MW mature IL-10. For immunoblots, typically, IL-1beta is detected from supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serum plus 10 ng/mL E. coli LPS. For immunoprecipitation a dilution of 1:400 to 1:800 is suggested. Pre-clearing the preparation with a non-specific rabbit IgG (011-001-297) to reduce background. For immunohistochemistry a dilution of 1:100 to 1:200 is suggested. Either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL- 1beta. For ELISA also use HRP Conjugated Anti-Rabbit IgG [HalphaL] (Goat) (611-1302) for detection.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling back to top
Supplier Images
 image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN799612) anti-Interleukin 1, beta (IL1B) (N-Term) antibody