|Antigen||Interleukin 1, beta (IL1B) Antibodies|
|Reactivity||Sheep (Ovine) Alternatives|
|Conjugate||This IL1B antibody is un-conjugated Alternatives|
Functional Studies (Func), Enzyme Immunoassay (EIA), Western Blotting (WB)
|1 reference available|
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Product Details anti-IL1B AntibodyTarget Details IL1B Application Details Handling References for anti-IL1B antibody (ABIN318984) Images
|Specificity||This antibody recognizes Interleukin-1 beta (IL-1 beta). No cross-reactivity is seen with ovine IL-6, IL-8, MCP-1 or TNF alpha.|
Species reactivity (expected):Bovine, Goat.
Species reactivity (tested):Sheep.
|Immunogen||Recombinant IL-1 beta|
Target Details IL1BProduct Details anti-IL1B Antibody Application Details Handling References for anti-IL1B antibody (ABIN318984) Images back to top
|Alternative Name||Interleukin-1 beta / IL-1B (IL1B Antibody Abstract)|
|Background||Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2|
|Pathways||NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy|
Application DetailsProduct Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B antibody (ABIN318984) Images back to top
ELISA: 1/500. Western blot. Functional assay: Neutralises the bioactivity of IL-1 beta. Removal of sodium azide isrecommended prior to use.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B antibody (ABIN318984) Images back to top
|Buffer||0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.|
References for anti-IL1B antibody (ABIN318984)Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Rothel, Hurst, Seow, Pépin, Berthon, Corner, Wood: "Analysis of ovine IL-1 beta production in vivo and in vitro by enzyme immunoassay and immunohistochemistry." in: Veterinary immunology and immunopathology, Vol. 57, Issue 3-4, pp. 267-78, 1997