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|Antigen||Interleukin 1 Receptor Antagonist (IL1RN) Antibodies|
|Reactivity||Human, Monkey Alternatives|
|Conjugate||This IL1RN antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|4 references available|
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Product Details anti-IL1RN AntibodyTarget Details IL1RN Application Details Handling References for anti-IL1RN antibody (ABIN116864) Images
|Immunogen||Prepared by repeated immunizations with recombinant human sIL-1Ra produced in E.coli.|
Target Details IL1RNProduct Details anti-IL1RN Antibody Application Details Handling References for anti-IL1RN antibody (ABIN116864) Images back to top
|Alternative Name||IL1RN / IL1RA (IL1RN Antibody Abstract)|
|Background||IL1RA is a member of the Interleukin 1 cytokine family. This protein inhibits the activities of Interleukin 1, alpha (IL1A) and Interleukin 1, beta (IL1B), and modulates a variety of Interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.Synonyms: ICIL-1RA, IL-1RN, IL-1ra, IL1 inhibitor, IL1F3, IRAP, Interleukin-1 receptor antagonist protein|
|Pathways||NF-kappaB Signaling, Hormone Transport|
Application DetailsProduct Details anti-IL1RN Antibody Target Details IL1RN Handling References for anti-IL1RN antibody (ABIN116864) Images back to top
This IgG fraction antibody of anti-Human IL-1 Receptor Antagonist has been tested for usein Neutralizations, ELISA, Radioimmunoassays, Western blotting, Immunohistochemistryand Flow cytometry. Reactivity in other immunoassays is unknown. Note: This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L]and TMB as a substrate. A working dilution range of 1: 400 to 1: 800 is suggested for thisapplication. This product has been assayed by ELISA against IL-1Receptor Antagonist usingHRP Conjugated Anti-Rabbit IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes atroom temperature. A working dilution range of 1: 500 to 1: 1,000 is suggested for thisproduct. For use in ELISA formats, this antibody is best used as the second antibody incombination with a monoclonal antibody as a capture antibody. This product has beenassayed by Immunohistochemistry. A dilution of 1: 400 or greater is suggested for thisimmunoassay. Either paraffin fixation or cryofixation can be used forImmunohistochemistry. This product has been assayed by radioimmunoassay againstantigen. A dilution of 1: 8,000 is suggested for this immunoassay. Recommended Dilutions: ELISA: 1/1,000-1/5,000. Western Blot: 1/500-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL1RN Antibody Target Details IL1RN Application Details References for anti-IL1RN antibody (ABIN116864) Images back to top
|Concentration||1.0 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store lyophilized product at 2-8 °C. After reconstitution, store at -20 °C.|
References for anti-IL1RN antibody (ABIN116864)Product Details anti-IL1RN Antibody Target Details IL1RN Application Details Handling Images back to top
Arend, Malyak, Guthridge, Gabay: "Interleukin-1 receptor antagonist: role in biology." in: Annual review of immunology, Vol. 16, pp. 27-55, 1998
Hammerberg, Arend, Fisher, Chan, Berger, Haskill, Voorhees, Cooper: "Interleukin-1 receptor antagonist in normal and psoriatic epidermis." in: The Journal of clinical investigation, Vol. 90, Issue 2, pp. 571-83, 1992
Haskill, Martin, Van Le, Morris, Peace, Bigler, Jaffe, Hammerberg, Sporn, Fong: "cDNA cloning of an intracellular form of the human interleukin 1 receptor antagonist associated with epithelium." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 9, pp. 3681-5, 1991
Eisenberg, Evans, Arend, Verderber, Brewer, Hannum, Thompson: "Primary structure and functional expression from complementary DNA of a human interleukin-1 receptor antagonist." in: Nature, Vol. 343, Issue 6256, pp. 341-6, 1990