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|Antigen||Insulin (INS) Antibodies|
|Reactivity||Cat (Feline), Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine) Alternatives|
|Conjugate||This Insulin antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|2 references available|
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Product Details anti-Insulin AntibodyTarget Details Insulin Application Details Handling References for anti-Insulin antibody (ABIN114751) Images
This antibody recognizes purified Insulin from the pancreas of Human, Bovine, Horse, Sheep as well as Proinsulin from Human. Cross reaction has been observed with insulin containing cells in fixed sections of pancreas from Human, Porcine, Dog, Rabbit, Bovine, Sheep, Rat, Guinea Pig and Cat.
Cellular Localization: Cytoplasmic.Cellular Localization
Target Details InsulinProduct Details anti-Insulin Antibody Application Details Handling References for anti-Insulin antibody (ABIN114751) Images back to top
|Alternative Name||Insulin (INS Antibody Abstract)|
|Background||Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.Synonyms: INS|
|Research Area||Neurology, Diabetes, Hormones|
|Pathways||NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation|
Application DetailsProduct Details anti-Insulin Antibody Target Details Insulin Handling References for anti-Insulin antibody (ABIN114751) Images back to top
Immunohistochemistry on Formalin/Paraffin Sections: Use a dilution of 1/25-1/50 in anABC method (30 minutes at room temperature). Recommended Positive Control: Pancreas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Insulin Antibody Target Details Insulin Application Details References for anti-Insulin antibody (ABIN114751) Images back to top
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-Insulin antibody (ABIN114751)Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Madsen: "Proinsulin-specific monoclonal antibodies. Immunocytochemical application as beta-cell markers and as probes for conversion." in: Diabetes, Vol. 36, Issue 10, pp. 1203-11, 1987
Keilacker, Dietz, Witt, Woltanski, Berling, Ziegler: "Kinetic properties of monoclonal insulin antibodies." in: Biomedica biochimica acta, Vol. 45, Issue 8, pp. 1093-102, 1986