anti-Human Insulin antibody for Immunocytochemistry

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
Reactivity
Cow (Bovine), Human, Mouse (Murine)
979
491
378
368
232
185
47
9
7
6
5
5
4
2
2
1
1
1
1
1
1
1
Host
Rat
811
195
50
15
3
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
53
43
38
28
27
22
22
19
17
17
15
14
14
14
14
14
14
14
9
9
9
8
8
8
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
2
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
496
388
278
272
245
233
189
117
106
67
66
31
26
23
21
7
7
6
5
4
4
3
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4900790
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN617877 IEM ICC IF IHC Rabbit IgG Log in to see Polyclonal 44
1 ABIN269653 DB ICC IF IHC (p) IHC RIA Mouse IgG1 Log in to see K36aC10 0
1 ABIN4326102 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN337249 FACS ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal 0
1 ABIN1172910 ICC IHC WB Rabbit IgG AA 25-110 Log in to see Polyclonal 0
1 ABIN4326030 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN94408 Func ICC IHC (p) RIA ELISA Mouse IgG1 Log in to see IN-05 1
1 ABIN206973 BR ELISA Func ICC IHC IHC (p) Neut RIA Mouse IgG1 Log in to see IN-05 0
1 ABIN4326033 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM531 0
1 ABIN4326058 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05) 0
1 ABIN4326109 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326114 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326111 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326112 FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326055 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04) 0
1 ABIN4326104 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326115 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326064 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4904068 ICC IF IHC Rabbit IgG Log in to see 0
1 ABIN1735946 ICC FACS IF IHC (p) ELISA Rabbit IgG Log in to see Polyclonal 0

General

Antigen Insulin (INS) Antibodies
Reactivity Cow (Bovine), Human, Mouse (Murine)
(979), (491), (378), (368), (232), (185), (47), (9), (7), (6), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rat
(811), (195), (50), (15), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(53), (43), (38), (28), (27), (22), (22), (19), (17), (17), (15), (14), (14), (14), (14), (14), (14), (14), (9), (9), (9), (8), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
(496), (388), (278), (272), (245), (233), (189), (117), (106), (67), (66), (31), (26), (23), (21), (7), (7), (6), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling References for anti-Insulin antibody (ABIN4900790) Images
Specificity Detects bovine, human, and mouse insulin.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen E. coli-derived recombinant human Insulin
Clone 182410
Isotype IgG2a
Plasmids, Primers & others

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling References for anti-Insulin antibody (ABIN4900790) Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Alternative Names: IDDM2, ILPR, INS, insulin, IRDN, MODY10, proinsulin
Gene ID 3630
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling References for anti-Insulin antibody (ABIN4900790) Images back to top
Application Notes Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details References for anti-Insulin antibody (ABIN4900790) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-Insulin antibody (ABIN4900790)

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Product cited in:

Cucak, Grunnet, Rosendahl: "Accumulation of M1-like macrophages in type 2 diabetic islets is followed by a systemic shift in macrophage polarization." in: Journal of leukocyte biology, Vol. 95, Issue 1, pp. 149-60, 2014 Method employed by authors: Flow Cytometry (FACS), Flow Cytometry (FACS) (Sample species: Mouse (Murine)).

Cucak, Mayer, Tonnesen, Thomsen, Grunnet, Rosendahl: "Macrophage contact dependent and independent TLR4 mechanisms induce β-cell dysfunction and apoptosis in a mouse model of type 2 diabetes." in: PLoS ONE, Vol. 9, Issue 3, pp. e90685, 2014 Method employed by authors: Flow Cytometry (FACS) (Sample species: Mouse (Murine)).

Kalis, Bolmeson, Esguerra, Gupta, Edlund, Tormo-Badia, Speidel, Holmberg, Mayans, Khoo, Wendt, Eliasson, Cilio: "Beta-cell specific deletion of Dicer1 leads to defective insulin secretion and diabetes mellitus." in: PLoS ONE, Vol. 6, Issue 12, pp. e29166, 2012 Method employed by authors: Immunocytochemistry (ICC), Immunocytochemistry (ICC) (Sample species: Mouse (Murine)).

Hagberg, Mehlem, Falkevall, Muhl, Fam, Ortsäter, Scotney, Nyqvist, Samén, Lu, Stone-Elander, Proietto, Andrikopoulos, Sjöholm, Nash, Eriksson: "Targeting VEGF-B as a novel treatment for insulin resistance and type 2 diabetes." in: Nature, Vol. 490, Issue 7420, pp. 426-30, 2012 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Jin, Li, Xu, Geng, He, Su, Tao, Ding, Wang, Hu: "Conversion of immortal liver progenitor cells into pancreatic endocrine progenitor cells by persistent expression of Pdx-1." in: Journal of cellular biochemistry, Vol. 104, Issue 1, pp. 224-36, 2008 Method employed by authors: Immunocytochemistry (ICC), Immunocytochemistry (ICC) (Sample species: Mouse (Murine)).

Dowling, ODriscoll, OSullivan, Dowd, Henry, Jeppesen, Meleady, Clynes: "Proteomic screening of glucose-responsive and glucose non-responsive MIN-6 beta cells reveals differential expression of proteins involved in protein folding, secretion and oxidative stress." in: Proteomics, Vol. 6, Issue 24, pp. 6578-87, 2006 Method employed by authors: Immunocytochemistry (ICC), Immunocytochemistry (ICC) (Sample species: Mouse (Murine)).

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling References for anti-Insulin antibody (ABIN4900790) back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-Insulin (INS) antibody (ABIN4900790) Insulin in beta TC-6 Mouse Cell Line. Insulin was detected in immersion fixed beta TC...