anti-Human Insulin antibody for Immunoprecipitation

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
Reactivity
Human, Pig (Porcine)
951
469
370
350
240
194
45
11
9
8
7
7
6
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Host
Mouse
785
197
50
9
4
2
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
52
41
36
21
20
20
20
15
15
15
13
13
13
13
13
13
13
10
9
9
7
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
462
358
276
270
259
237
180
118
106
66
66
31
25
23
21
8
7
6
5
4
4
3
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN5556681
$ 619.30
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN129507 IP IHC ELISA WB Mouse IgG Log in to see 2D11-H5 0
1 ABIN1107760 IHC (p) IP Mouse IgG1 Log in to see ISL-8J 1
1 ABIN4326102 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326030 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN2742155 ELISA FACS IF IHC IHC (p) IP WB Mouse IgG1, kappa AA 1-84 Log in to see 2D11-H5 or INS05 0
1 ABIN4326104 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326111 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326112 FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326114 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326115 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326055 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04) 0
1 ABIN4326058 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05) 0
1 ABIN4326033 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM531 0
1 ABIN4326109 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326064 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN2742156 ELISA FACS IF IHC IHC (p) IP WB Mouse IgG1, kappa AA 1-84 Log in to see E2-E3 or INS04 0
1 ABIN2742157 FACS IF IHC IHC (p) IP WB Mouse IgG1, kappa AA 1-84 Log in to see E2-E3-2D11-H5 or INS04-INS05 0
1 ABIN4904069 IF IHC IP Mouse IgG Log in to see 0
1 ABIN2294343 FACS IF IP IHC ELISA WB Mouse IgG1 kappa AA 1-84 Log in to see 2D11-H5 0
1 ABIN4326118 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see SPM531 0

General

Antigen Insulin (INS) Antibodies
Reactivity Human, Pig (Porcine)
(951), (469), (370), (350), (240), (194), (45), (11), (9), (8), (7), (7), (6), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(785), (197), (50), (9), (4), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(52), (41), (36), (21), (20), (20), (20), (15), (15), (15), (13), (13), (13), (13), (13), (13), (13), (10), (9), (9), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(462), (358), (276), (270), (259), (237), (180), (118), (106), (66), (66), (31), (25), (23), (21), (8), (7), (6), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling Images
Sequence Human Proinsulin (precursor) 21 aa A chain 90-110. 30 aa B chain 25-54. 1 MALWMRLLPL LALLALWGPD PAAAFVNQHL CGSHLVEALY LVCGERGFFY TPKTRREAED 61 LQVGQVELGG GPGAGSLQPL ALEGSLQKRG IVEQCCTSIC SLYQLENYCN
Specificity This monoclonal 2D11.H5 antibody recognizes a polypeptide which is identified as Insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. This protein A purified Mouse monoclonal antibody reacts specifically with Insulin from Human and Swine sources. Cross reactivity with Insulin from Mouse and Rat does not occur. Cross reactivity with Insulin from other sources has not been determined.
Cellular Localization: Cytoplasmic.Cellular Localization:
Characteristics Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)
Purification Protein A Chromatography
Immunogen Purified human Insulin coupled to Bovine Serum Albumin (BSA).
Clone 2D11-H5
Isotype IgG1
Plasmids, Primers & others

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-peptide basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain acids. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as β-cell and tumor (insulinoma) markers.Synonyms: INS
Molecular Weight 6 kDa
Gene ID 3630
NCBI Accession NP_000198
UniProt P01308
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
Application Notes This antibody is suitable for ELISA, Immunohistochemistry, Immunoblotting andimmunoprecipitation. Insulin has a cytoplasmic localization. Pancreatic tissue or β-cellsfrom islets of Langerhans can be used as a positive control. Generally a 1: 50 to 1: 200 dilution is suitable for most applications. A 30 to 60 minuteincubation time is usually sufficient. However, depending upon the fixation conditions andthe staining system employed, optimal incubation should be determined by the user. Frozen sections of paraffin embedded tissues are recommended for preparations to use forimmunohistochemistry. No special pretreatment is required. Researchers shoulddetermine the optimal dilution for other specific assays and cell types. ELISA: Results of Mab anti-Insulin antibody were tested against human insulin by ELISA. Each well was coated with 0.1g of conjugate. The starting concentration of antibody in thedilution series was 10 μg/mL. Each point on the Y-axis represents a 3-fold dilution. Themidpoint of the titration curve represents approx-imately 5 ng/mL antibody or a 1: 200,000dilution from the stock concentration. HRP conjugated Gt-a- Mouse IgG H&L and TMBsubstrate were used for detection (see figure 1).
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage -20 °C
Storage Comment Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.