anti-Pig (Porcine) Insulin antibody for Flow Cytometry

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
Reactivity
Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
948
464
363
347
237
191
45
11
9
8
7
7
6
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Host
Mouse
779
201
49
9
4
2
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is conjugated to PerCP
52
40
36
21
20
20
19
15
15
14
13
13
13
13
13
13
13
9
9
9
7
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
458
353
268
266
253
232
174
111
105
66
64
35
25
23
21
7
5
4
4
4
4
3
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN6157476
$ 392.62
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN6157477 FACS IF IHC (f) PE Mouse IgG1, kappa Log in to see IRDN-794 0
7 ABIN6157469 FACS IF IHC (f) APC Mouse IgG1, kappa Log in to see IRDN-794 0
7 ABIN6157480 FACS IF IHC (f) Mouse IgG1, kappa Log in to see IRDN-794 0
4 ABIN6157355 FACS IF IHC (f) APC Mouse IgG1, kappa Log in to see 2D11-H5 0
4 ABIN6157362 FACS IF IHC (f) PerCP Mouse IgG1, kappa Log in to see 2D11-H5 0
4 ABIN6157363 FACS IF IHC (f) PE Mouse IgG1, kappa Log in to see 2D11-H5 0
4 ABIN6157438 FACS IF IHC (f) PerCP Mouse IgG1, kappa Log in to see E2-E3 0
4 ABIN6157439 FACS IF IHC (f) PE Mouse IgG1, kappa Log in to see E2-E3 0
4 ABIN6157514 FACS IF IHC (f) IHC (p) ISt PerCP Mouse IgG1, kappa Log in to see IRDN-805 0
4 ABIN6157507 FACS IF IHC (f) IHC (p) ISt APC Mouse IgG1, kappa Log in to see IRDN-805 0
4 ABIN6157515 FACS IF IHC (f) IHC (p) ISt PE Mouse IgG1, kappa Log in to see IRDN-805 0
4 ABIN6157431 FACS IF IHC (f) APC Mouse IgG1, kappa Log in to see E2-E3 0
4 ABIN6157366 FACS IF IHC (f) Mouse IgG1, kappa Log in to see 2D11-H5 0
4 ABIN6157442 FACS IF IHC (f) Mouse IgG1, kappa Log in to see E2-E3 0
4 ABIN6157518 FACS IF IHC (f) IHC (p) ISt Mouse IgG1, kappa Log in to see IRDN-805 0
1 ABIN6157333 FACS IF IHC (f) CF®640R Mouse IgG1, kappa Log in to see 2D11-H5 0
1 ABIN6157341 FACS IF IHC (f) CF®660R Mouse IgG1, kappa Log in to see 2D11-H5 0
1 ABIN6157343 FACS IF IHC (f) CF®568 Mouse IgG1, kappa Log in to see 2D11-H5 0
1 ABIN6157353 FACS IF IHC (f) CF®594 Mouse IgG1, kappa Log in to see 2D11-H5 0
1 ABIN6157349 FACS IF IHC (f) CF®680R Mouse IgG1, kappa Log in to see 2D11-H5 0

General

Antigen Insulin (INS) Antibodies
Reactivity Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(948), (464), (363), (347), (237), (191), (45), (11), (9), (8), (7), (7), (6), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(779), (201), (49), (9), (4), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is conjugated to PerCP
(52), (40), (36), (21), (20), (20), (19), (15), (15), (14), (13), (13), (13), (13), (13), (13), (13), (9), (9), (9), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
(458), (353), (268), (266), (253), (232), (174), (111), (105), (66), (64), (35), (25), (23), (21), (7), (5), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling Images
Purpose Mouse Monoclonal anti-Insulin (IRDN/794), Per-CP Conjugate
Characteristics This antibody recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Recombinant INS protein
Clone IRDN-794
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background IDDM2, ILPR, IRDN, MODY10, Proinsulin
Molecular Weight 6 kDa
Gene ID 3630, 272259
UniProt P01308
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
Application Notes Immunohistology formalin-fixed 0.1-0.2 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Immunofluorescence 1-2 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

MIA PaCa-2 cells or Pancreas

Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling back to top
Supplier Images
 image for anti-Insulin (INS) antibody (PerCP) (ABIN6157476) anti-Insulin (INS) antibody (PerCP)
 image for anti-Insulin (INS) antibody (PerCP) (ABIN6157476) anti-Insulin (INS) antibody (PerCP) (Image 2)