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|Antigen||Insulin (INS) Antibodies|
|Reactivity||Cow (Bovine), Human, Pig (Porcine) Alternatives|
|Conjugate||This Insulin antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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Product Details anti-Insulin AntibodyTarget Details Insulin Application Details Handling Images
|Specificity||The antibody recognizes Insulin and recombinant Proinsulin, but does not react with synthetic C-Peptide.|
|Purification||Affinity Chromatography on Protein G.|
|Immunogen||Recombinant full length Insulin expressed in Yeast.|
Target Details InsulinProduct Details anti-Insulin Antibody Application Details Handling Images back to top
|Alternative Name||Insulin (INS Antibody Abstract)|
|Background||Proinsulin is a prohormone precursor to Insulin, made by the pancreas in response to increased blood sugar. Proinsulin is 39 amino acids longer than the mature Insulin, of which 4 are completely lost and the remaining 35 forms the C-peptide. The measurement of Proinsulin in serum provides a useful, valuable information for the diagnosis of insulinomas. Proinsulin levels have also been shown to be elevated in non-insulin dependent diabetics (NIDDM), in insulin dependent diabetics (IDDM) and other clinical situations.Synonyms: INS|
|Pathways||NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation|
Application DetailsProduct Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
Indirect ELISA: Use at 2 μg/mL (For detection of antigen). Western Blot: Use at 4-5 μg/mL. Immunohistochemistry: Use at 20-40 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
|Buffer||PBS containing containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.|
|Precaution of Use||This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store the antibody (in aliquots) at -20 °CAvoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-Insulin Antibody Target Details Insulin Application Details Handling back to top