anti-Pig (Porcine) Insulin antibody for Western Blotting

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
Reactivity
Cow (Bovine), Human, Pig (Porcine)
979
490
379
368
232
185
47
9
7
6
5
5
4
2
2
1
1
1
1
1
1
1
Host
Mouse
810
195
50
16
3
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
53
43
38
28
27
22
22
19
17
17
15
14
14
14
14
14
14
14
9
9
9
8
8
8
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
496
388
279
272
245
232
188
118
106
67
66
31
25
23
21
8
7
6
5
4
4
3
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN487416
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.168048 ABIN1859457 ICC IHC WB Rabbit IgG AA 88-108 Log in to see Polyclonal 0
3.168048 ABIN1859458 ICC IHC WB Rabbit IgG AA 25-54 Log in to see Polyclonal 0
1 ABIN5556681 EIA IHC (fro) IP WB Mouse IgG1 Log in to see 2D11-H5 0
1 ABIN4326102 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN272321 EIA IHC (p) WB Mouse IgG1 Log in to see E2E3 3
1 ABIN4326030 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN2742155 ELISA FACS IF IHC IHC (p) IP WB Mouse IgG1, kappa AA 1-84 Log in to see 2D11-H5 or INS05 0
1 ABIN602181 IHC ELISA WB Mouse IgG1 Log in to see 0
1 ABIN4326033 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM531 0
1 ABIN4326058 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05) 0
1 ABIN4326109 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326114 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326111 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326112 FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326055 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04) 0
1 ABIN4326104 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326115 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN4326064 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN1832566 ELISA WB FITC Guinea Pig IgG Log in to see Polyclonal 0
1 ABIN2610272 WB FITC Rabbit IgG AA 25-54 Log in to see Polyclonal 0

General

Antigen Insulin (INS) Antibodies
Reactivity Cow (Bovine), Human, Pig (Porcine)
(979), (490), (379), (368), (232), (185), (47), (9), (7), (6), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(810), (195), (50), (16), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(53), (43), (38), (28), (27), (22), (22), (19), (17), (17), (15), (14), (14), (14), (14), (14), (14), (14), (9), (9), (9), (8), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(496), (388), (279), (272), (245), (232), (188), (118), (106), (67), (66), (31), (25), (23), (21), (8), (7), (6), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling Images
Specificity The antibody recognizes Insulin and recombinant Proinsulin, but does not react with synthetic C-Peptide.
Characteristics Synonyms: INS
Purification Affinity Chromatography on Protein G.
Immunogen Recombinant full length Insulin expressed in Yeast.
Clone HPI-B5
Isotype IgG1
Plasmids, Primers & others

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Proinsulin is a prohormone precursor to Insulin, made by the pancreas in response to increased blood sugar. Proinsulin is 39 amino acids longer than the mature Insulin, of which 4 are completely lost and the remaining 35 forms the C-peptide. The measurement of Proinsulin in serum provides a useful, valuable information for the diagnosis of insulinomas. Proinsulin levels have also been shown to be elevated in non-insulin dependent diabetics (NIDDM), in insulin dependent diabetics (IDDM) and other clinical situations.Synonyms: INS
Gene ID 3630
UniProt P01308
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
Application Notes Indirect ELISA: Use at 2 μg/mL (For detection of antigen). Western Blot: Use at 4-5 μg/mL. Immunohistochemistry: Use at 20-40 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
Concentration 0.5 mg/mL
Buffer PBS containing containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °CAvoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Insulin (INS) antibody (ABIN487416) anti-Insulin (INS) antibody
Western Blotting (WB) image for anti-Insulin (INS) antibody (ABIN487416) anti-Insulin (INS) antibody (Image 2)