anti-Human Integrin beta 2 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • CD18
  • LAD
  • LCAMB
  • LFA-1
  • MAC-1
  • MF17
  • MFI7
  • 2E6
  • AI528527
  • Cd18
  • Lfa1
  • ITGB2
  • itgb2
  • itgb2-a
  • itgb2-b
  • integrin subunit beta 2
  • integrin beta 2
  • integrin subunit beta 2 S homeolog
  • ITGB2
  • Itgb2
  • itgb2.S
Epitope
AA 534-546
46
36
27
21
16
15
11
11
7
6
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
504
198
119
38
24
21
8
4
4
3
3
3
3
3
2
1
1
Host
Mouse
350
174
144
9
3
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is conjugated to PE
81
67
48
26
12
11
8
8
7
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
447
286
177
172
93
89
75
72
65
40
26
15
13
10
7
6
3
1
1
1
1
1
1
1
1
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Catalog No. ABIN192094
$ 366.85
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN94008 FACS Func IHC (f) IHC (p) IP WB FITC Mouse IgG1 AA 534-546 Log in to see MEM-48 20
1 ABIN94007 FACS Func IHC (f) IHC (p) IP WB Biotin Mouse IgG1 AA 534-546 Log in to see MEM-48 20
1 ABIN125688 FACS Func IHC (f) IHC (p) IP WB APC Mouse IgG1 AA 534-546 Log in to see MEM-48 19
1 ABIN94005 FACS Func IHC (f) IHC (p) IP WB Mouse IgG1 AA 534-546 Log in to see MEM-48 19
1 ABIN2749019 Func IHC (f) FACS IP IHC (p) WB Mouse IgG1 Log in to see MEM-48 12

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Epitope AA 534-546
(46), (36), (27), (21), (16), (15), (11), (11), (7), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(504), (198), (119), (38), (24), (21), (8), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1)
Host Mouse
(350), (174), (144), (9), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is conjugated to PE
(81), (67), (48), (26), (12), (11), (8), (8), (7), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(447), (286), (177), (172), (93), (89), (75), (72), (65), (40), (26), (15), (13), (10), (7), (6), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 20 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 antibody (ABIN192094) Images
Specificity The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit, beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
No Cross-Reactivity Dog (Canine)
Characteristics The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Immunogen Leukocytes of a patient suffering from a LGL-type leukemia.
Clone MEM-48
Isotype IgG1
Plasmids, Primers & others

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 antibody (ABIN192094) Images back to top
Antigen
Alternative Name ITGB2 (ITGB2 Antibody Abstract)
Background CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Pathways NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 antibody (ABIN192094) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 antibody (ABIN192094) Images back to top
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-Integrin beta 2 antibody (ABIN192094)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Background publications

Tran, Glass, Uzel, Darnell, Spalding, Holland, Shevach: "Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 5, pp. 2929-38, 2009

Balashova, Shah, Patel, Megalla, Kachlany: "Aggregatibacter actinomycetemcomitans LtxC is required for leukotoxin activity and initial interaction between toxin and host cells." in: Gene, Vol. 443, Issue 1-2, pp. 42-7, 2009

Cera, Fabbri, Molendini, Corada, Orsenigo, Rehberg, Reichel, Krombach, Pardi, Dejana: "JAM-A promotes neutrophil chemotaxis by controlling integrin internalization and recycling." in: Journal of cell science, Vol. 122, Issue Pt 2, pp. 268-77, 2009

Nakayama, Yoshizaki, Prinetti, Sonnino, Mauri, Takamori, Ogawa, Iwabuchi: "Lyn-coupled LacCer-enriched lipid rafts are required for CD11b/CD18-mediated neutrophil phagocytosis of nonopsonized microorganisms." in: Journal of leukocyte biology, Vol. 83, Issue 3, pp. 728-41, 2008

Sewald, Gebert-Vogl, Prassl, Barwig, Weiss, Fabbri, Osicka, Schiemann, Busch, Semmrich, Holzmann, Sebo, Haas: "Integrin subunit CD18 Is the T-lymphocyte receptor for the Helicobacter pylori vacuolating cytotoxin." in: Cell host & microbe, Vol. 3, Issue 1, pp. 20-9, 2008

Morova, Osicka, Masin, Sebo: "RTX cytotoxins recognize beta2 integrin receptors through N-linked oligosaccharides." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 14, pp. 5355-60, 2008

Zemani, Silvestre, Fauvel-Lafeve, Bruel, Vilar, Bieche, Laurendeau, Galy-Fauroux, Fischer, Boisson-Vidal: "Ex vivo priming of endothelial progenitor cells with SDF-1 before transplantation could increase their proangiogenic potential." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 28, Issue 4, pp. 644-50, 2008

Dassanayake, Maheswaran, Srikumaran: "Monomeric expression of bovine beta2-integrin subunits reveals their role in Mannheimia haemolytica leukotoxin-induced biological effects." in: Infection and immunity, Vol. 75, Issue 10, pp. 5004-10, 2007

Peters, Sindrilaru, Wang, Oreshkova, Renkl, Kess, Scharffetter-Kochanek: "CD18 in monogenic and polygenic inflammatory processes of the skin." in: The journal of investigative dermatology. Symposium proceedings / the Society for Investigative Dermatology, Inc. [and] European Society for Dermatological Research, Vol. 11, Issue 1, pp. 7-15, 2006

Solovjov, Pluskota, Plow: "Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2." in: The Journal of biological chemistry, Vol. 280, Issue 2, pp. 1336-45, 2005

Kess, Peters, Zamek, Wickenhauser, Tawadros, Loser, Varga, Grabbe, Nischt, Sunderkötter, Müller, Krieg, Scharffetter-Kochanek: "CD4+ T cell-associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 11, pp. 5697-706, 2003

Drbal, Angelisová, Cerný, Pavlistová, Cebecauer, Novák, Horejsí: "Human leukocytes contain a large pool of free forms of CD18." in: Biochemical and biophysical research communications, Vol. 275, Issue 2, pp. 295-9, 2000

Ottonello, Epstein, Dapino, Barbera, Morone, Dallegri et al.: "Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b ..." in: Blood, Vol. 93, Issue 10, pp. 3505-11, 1999

Shang, Issekutz: "Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers." in: European journal of immunology, Vol. 28, Issue 6, pp. 1970-9, 1998

Gao, Issekutz: "Mac-1 (CD11b/CD18) is the predominant beta 2 (CD18) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers." in: Immunology, Vol. 88, Issue 3, pp. 463-70, 1996

Product cited in:

Kuttruff, Koch, Kelp, Pawelec, Rammensee, Steinle: "NKp80 defines and stimulates a reactive subset of CD8 T cells." in: Blood, Vol. 113, Issue 2, pp. 358-69, 2009

Garnotel, Rittié, Poitevin, Monboisse, Nguyen, Potron, Maquart, Randoux, Gillery: "Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 11, pp. 5928-34, 2000

Stefanová, Horejsí: "Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 5, pp. 1587-92, 1991

Bazil, Stefanová, Hilgert, Kristofová, Van?k, Horejsí: "Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein." in: Folia biologica, Vol. 36, Issue 1, pp. 41-50, 1990

Larson, Springer: "Structure and function of leukocyte integrins." in: Immunological reviews, Vol. 114, pp. 181-217, 1990

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 antibody (ABIN192094) back to top
Supplier Images
Western Blotting (WB) image for anti-Integrin beta 2 (ITGB2) (AA 534-546) antibody (PE) (ABIN192094) Fig. A Flow Cytometry analysis. Surface staining of human peripheral blood cells with...
Western Blotting (WB) image for anti-Integrin beta 2 (ITGB2) (AA 534-546) antibody (PE) (ABIN192094) Western Blotting analysis (non-reducing conditions) of whole cell lysate using anti-h...
Immunohistochemistry (IHC) image for anti-Integrin beta 2 (ITGB2) (AA 534-546) antibody (PE) (ABIN192094) Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (Co...