anti-Human Integrin beta 2 antibody for Western Blotting

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • CD18
  • LAD
  • LCAMB
  • LFA-1
  • MAC-1
  • MF17
  • MFI7
  • 2E6
  • AI528527
  • Cd18
  • Lfa1
  • ITGB2
  • itgb2
  • itgb2-a
  • itgb2-b
  • integrin subunit beta 2
  • integrin beta 2
  • integrin subunit beta 2 S homeolog
  • ITGB2
  • Itgb2
  • itgb2.S
Reactivity
Human
473
207
117
40
38
21
20
13
5
4
3
3
3
3
3
2
1
1
Host
Mouse
361
160
155
11
3
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
83
73
50
27
14
11
10
8
8
8
7
6
6
6
6
6
6
5
5
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
Application
Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
470
285
191
147
95
93
76
71
54
40
28
17
10
9
7
6
4
2
1
1
1
1
1
1
1
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Catalog No. ABIN2749019
$ 253.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2281573 ABIN2749020 Func FACS IP WB Mouse IgG1 Log in to see MEM-148 10
3.2281573 ABIN192094 FACS Func IHC (f) IHC (p) IP WB PE Mouse IgG1 AA 534-546 Log in to see MEM-48 20
3.2281573 ABIN94007 FACS Func IHC (f) IHC (p) IP WB Biotin Mouse IgG1 AA 534-546 Log in to see MEM-48 20
3.2281573 ABIN94008 FACS Func IHC (f) IHC (p) IP WB FITC Mouse IgG1 AA 534-546 Log in to see MEM-48 20
3.2281573 ABIN125688 FACS Func IHC (f) IHC (p) IP WB APC Mouse IgG1 AA 534-546 Log in to see MEM-48 19
3.2281573 ABIN94005 FACS Func IHC (f) IHC (p) IP WB Mouse IgG1 AA 534-546 Log in to see MEM-48 19
3.2281573 ABIN2705756 WB Rabbit Center Log in to see Polyclonal 0
3.2281573 ABIN125714 FACS Func IP WB APC Mouse IgG1 Log in to see MEM-148 10
3.2281573 ABIN192259 FACS Func IP WB PE Mouse IgG1 Log in to see MEM-148 10
3.2281573 ABIN1531540 IF ELISA WB Rabbit IgG AA 720-769, pThr758 Log in to see Polyclonal 2
3.2281573 ABIN3043260 ICC IHC (p) WB Rabbit IgG AA 404-769 Log in to see Polyclonal 0
3.2281573 ABIN94010 Func FACS IP WB Mouse IgG1 Log in to see MEM-148 8
3.2281573 ABIN269112 CyTOF FACS Func IP WB Mouse IgG1 Log in to see MEM-148 0
3.2281573 ABIN3044225 IHC (p) WB Rabbit IgG AA 732-748, C-Term Log in to see Polyclonal 0
3.2281573 ABIN3043698 IHC (p) WB Rabbit IgG AA 24-58, N-Term Log in to see Polyclonal 0
3.2281573 ABIN1873315 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.2281573 ABIN1539259 WB Rabbit Ig AA 272-300, N-Term Log in to see Polyclonal 0
3.2281573 ABIN1859503 ICC IHC WB Rabbit IgG AA 451-627 Log in to see Polyclonal 0
3.2281573 ABIN1859504 ICC IHC WB Rabbit IgG AA 124-363 Log in to see Polyclonal 0
3.2281573 ABIN1882257 WB Mouse IgG1 Log in to see 534CT6-2-1 5

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Reactivity Human
(473), (207), (117), (40), (38), (21), (20), (13), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1)
Host Mouse
(361), (160), (155), (11), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is un-conjugated
(83), (73), (50), (27), (14), (11), (10), (8), (8), (8), (7), (6), (6), (6), (6), (6), (6), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(470), (285), (191), (147), (95), (93), (76), (71), (54), (40), (28), (17), (10), (9), (7), (6), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 12 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 antibody (ABIN2749019) Images
Specificity The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit, beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
No Cross-Reactivity Dog (Canine)
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
Immunogen Leukocytes of a patient suffering from a LGL-type leukemia.
Clone MEM-48
Isotype IgG1

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 antibody (ABIN2749019) Images back to top
Antigen
Alternative Name CD18 (ITGB2 Antibody Abstract)
Background CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Pathways NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 antibody (ABIN2749019) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 antibody (ABIN2749019) Images back to top
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-Integrin beta 2 antibody (ABIN2749019)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Product cited in:

Kuttruff, Koch, Kelp, Pawelec, Rammensee, Steinle: "NKp80 defines and stimulates a reactive subset of CD8 T cells." in: Blood, Vol. 113, Issue 2, pp. 358-69, 2009

Garnotel, Rittié, Poitevin, Monboisse, Nguyen, Potron, Maquart, Randoux, Gillery: "Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 11, pp. 5928-34, 2000

Stefanová, Horejsí: "Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 5, pp. 1587-92, 1991

Bazil, Stefanová, Hilgert, Kristofová, Van?k, Horejsí: "Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein." in: Folia biologica, Vol. 36, Issue 1, pp. 41-50, 1990

Larson, Springer: "Structure and function of leukocyte integrins." in: Immunological reviews, Vol. 114, pp. 181-217, 1990

Background publications

Dassanayake, Maheswaran, Srikumaran: "Monomeric expression of bovine beta2-integrin subunits reveals their role in Mannheimia haemolytica leukotoxin-induced biological effects." in: Infection and immunity, Vol. 75, Issue 10, pp. 5004-10, 2007

Peters, Sindrilaru, Wang, Oreshkova, Renkl, Kess, Scharffetter-Kochanek: "CD18 in monogenic and polygenic inflammatory processes of the skin." in: The journal of investigative dermatology. Symposium proceedings / the Society for Investigative Dermatology, Inc. [and] European Society for Dermatological Research, Vol. 11, Issue 1, pp. 7-15, 2006

Solovjov, Pluskota, Plow: "Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2." in: The Journal of biological chemistry, Vol. 280, Issue 2, pp. 1336-45, 2005

Kess, Peters, Zamek, Wickenhauser, Tawadros, Loser, Varga, Grabbe, Nischt, Sunderkötter, Müller, Krieg, Scharffetter-Kochanek: "CD4+ T cell-associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 11, pp. 5697-706, 2003

Drbal, Angelisová, Cerný, Pavlistová, Cebecauer, Novák, Horejsí: "Human leukocytes contain a large pool of free forms of CD18." in: Biochemical and biophysical research communications, Vol. 275, Issue 2, pp. 295-9, 2000

Shang, Issekutz: "Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers." in: European journal of immunology, Vol. 28, Issue 6, pp. 1970-9, 1998

Gao, Issekutz: "Mac-1 (CD11b/CD18) is the predominant beta 2 (CD18) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers." in: Immunology, Vol. 88, Issue 3, pp. 463-70, 1996

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 antibody (ABIN2749019) back to top
Supplier Images
Western Blotting (WB) image for anti-Integrin beta 2 (ITGB2) antibody (ABIN2749019) Fig. A Flow Cytometry analysis. Surface staining of human peripheral blood cells with...
Western Blotting (WB) image for anti-Integrin beta 2 (ITGB2) antibody (ABIN2749019) Western Blotting analysis (non-reducing conditions) of whole cell lysate using anti-h...
Immunohistochemistry (IHC) image for anti-Integrin beta 2 (ITGB2) antibody (ABIN2749019) Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (Co...