anti-Mouse (Murine) Integrin beta 2 antibody for Functional Studies

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • CD18
  • LAD
  • LCAMB
  • LFA-1
  • MAC-1
  • MF17
  • MFI7
  • 2E6
  • AI528527
  • Cd18
  • Lfa1
  • ITGB2
  • itgb2
  • itgb2-a
  • itgb2-b
  • integrin subunit beta 2
  • integrin beta 2
  • integrin subunit beta 2 S homeolog
  • ITGB2
  • Itgb2
  • itgb2.S
Reactivity
Mouse (Murine)
521
201
120
35
23
21
9
4
4
3
3
3
3
3
2
1
1
Host
Rat
366
178
143
9
3
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
82
71
49
27
12
12
9
9
7
5
5
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
462
296
179
178
91
89
76
71
62
39
27
15
13
10
7
6
3
1
1
1
1
1
1
1
1
Options
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Available images

Catalog No. ABIN457404
$ 325.91
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
24.073498 ABIN457348 FACS Func IHC (f) IHC (p) IP WB FITC Rat IgG2a Log in to see M18-2 9
24.073498 ABIN457430 FACS Func IHC (f) IHC (p) IP WB Rat IgG2a Log in to see M18-2 8
16.573498 ABIN2191828 Func IA FACS WB Rat Log in to see 5G11 3
10 ABIN1176975 BR Func IHC (f) IHC (fro) FACS Rat IgG2a, kappa Log in to see M18-2 6
1 ABIN490178 BR FACS Func IHC IHC (fro) IHC (p) IP Neut WB Rat IgG2a Log in to see M18-2 0
1 ABIN490150 BR FACS Func IHC IHC (fro) IP Neut WB FITC Rat IgG2a Log in to see M18-2 0
1 ABIN490166 BR FACS Func IHC IHC (fro) IP Neut WB Rat IgG2a Log in to see M18-2 0
1 ABIN3199542 FACS Func IHC IP WB Rat IgG2a Log in to see M18-2 0
1 ABIN3199544 FACS Func IHC IP WB FITC Rat IgG2a Log in to see M18-2 0
1 ABIN3199543 FACS Func IHC IP WB Rat IgG2a Log in to see M18-2 0
1 ABIN2567290 Func FACS Rat IgG2a kappa Log in to see M18-2 0
1 ABIN2567291 Func FACS Rat IgG2a kappa Log in to see M18-2 0
1 ABIN5581273 BP FACS Func IHC IP WB Rat IgG2a Log in to see M18-2 0
-6.515954 ABIN5682076 FACS Func IHC IHC (fro) IHC (p) IP WB Rat IgG2a Log in to see M18-2 0

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Reactivity Mouse (Murine)
(521), (201), (120), (35), (23), (21), (9), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1)
Host Rat
(366), (178), (143), (9), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is un-conjugated
(82), (71), (49), (27), (12), (12), (9), (9), (7), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(462), (296), (179), (178), (91), (89), (76), (71), (62), (39), (27), (15), (13), (10), (7), (6), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 antibody (ABIN457404) Images
Specificity The rat monoclonal antibody M18/2 recognizes CD18 antigen (integrin beta2 subunit, beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.
No Cross-Reactivity Dog (Canine)
Cross-Reactivity (Details) Mouse
Purification Purified by protein-G affinity chromatography
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Immunogen Murine cytotoxic T cell glycoproteins
Clone M18-2
Isotype IgG2a
Plasmids, Primers & others

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 antibody (ABIN457404) Images back to top
Antigen
Alternative Name CD18 (ITGB2 Antibody Abstract)
Background CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts.
Pathways NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 antibody (ABIN457404) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 antibody (ABIN457404) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 μ,m filter sterilized.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-Integrin beta 2 antibody (ABIN457404)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Product cited in:

Cullere, Lauterbach, Tsuboi, Mayadas: "Neutrophil-selective CD18 silencing using RNA interference in vivo." in: Blood, Vol. 111, Issue 7, pp. 3591-8, 2008

Varga, Balkow, Wild, Stadtbaeumer, Krummen, Rothoeft, Higuchi, Beissert, Wethmar, Scharffetter-Kochanek, Vestweber, Grabbe: "Active MAC-1 (CD11b/CD18) on DCs inhibits full T-cell activation." in: Blood, Vol. 109, Issue 2, pp. 661-9, 2007

Watts, Beurskens, Martin-Padura, Ballantyne, Klickstein, Brenner, Lee: "Manifestations of inflammatory arthritis are critically dependent on LFA-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 6, pp. 3668-75, 2005

Barlow, Langston, Matthews, Chidlow, Kevil: "CD18 deficiency protects against multiple low-dose streptozotocin-induced diabetes." in: The American journal of pathology, Vol. 165, Issue 6, pp. 1849-52, 2004

Sakurai, Taguchi, Anand, Ambati, Gragoudas, Miller, Adamis, Ambati: "Targeted disruption of the CD18 or ICAM-1 gene inhibits choroidal neovascularization." in: Investigative ophthalmology & visual science, Vol. 44, Issue 6, pp. 2743-9, 2003

Abraham, Miller: "Molecular mechanisms of IL-2 gene regulation following costimulation through LFA-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 9, pp. 5193-201, 2001

Avni, Pur, Yefenof, Baniyash: "Complement receptor 3 of macrophages is associated with galectin-1-like protein." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 12, pp. 6151-8, 1998

Zahalka, Naor: "Beta 2-integrin dependent aggregate formation between LB T cell lymphoma and spleen cells: assessment of correlation with spleen invasiveness." in: International immunology, Vol. 6, Issue 6, pp. 917-24, 1994

Zahalka, Okon, Naor: "Blocking lymphoma invasiveness with a monoclonal antibody directed against the beta-chain of the leukocyte adhesion molecule (CD18)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 10, pp. 4466-77, 1993

Sanchez-Madrid, Simon, Thompson, Springer: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." in: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, 1983

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 antibody (ABIN457404) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin beta 2 (ITGB2) antibody (ABIN457404) Surface staining of mouse splenocytes using anti-CD18 antibody (clone M18/2).