anti-Mouse (Murine) Integrin beta 2 antibody for Western Blotting

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • CD18
  • LAD
  • LCAMB
  • LFA-1
  • MAC-1
  • MF17
  • MFI7
  • 2E6
  • AI528527
  • Cd18
  • Lfa1
  • ITGB2
  • itgb2
  • itgb2-a
  • itgb2-b
  • integrin subunit beta 2
  • integrin beta 2
  • integrin subunit beta 2 S homeolog
  • ITGB2
  • Itgb2
  • itgb2.S
Epitope
AA 720-769, pThr758
46
35
28
21
16
15
11
11
7
6
5
5
5
5
5
5
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
504
197
118
38
24
21
8
4
4
3
3
3
3
3
2
1
1
Host
Rabbit
351
173
144
9
3
1
Clonality
Polyclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
81
68
48
26
12
11
8
8
7
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
448
286
178
172
93
88
74
73
66
40
26
15
13
11
7
6
3
1
1
1
1
1
1
1
1
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Catalog No. ABIN1531540
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1357207 ABIN6262689 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1357207 ABIN1873315 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1357207 ABIN1859502 ICC IHC WB Rabbit IgG AA 431-652 Log in to see Polyclonal 0
3.1357207 ABIN2973629 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1357207 ABIN5964587 WB Rabbit IgG Log in to see Polyclonal 0
3.1357207 ABIN3023118 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1357207 ABIN3031374 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
3.1357207 ABIN3031376 IHC (p) WB Rabbit IgG AA 17-382 Log in to see Polyclonal 0
3.1357207 ABIN2935025 IF/ICC IHC IP WB Rabbit AA 431-652 Log in to see Polyclonal 0
3.1357207 ABIN2430321 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN457404 FACS Func IHC (f) IHC (p) IP WB Rat IgG2a Log in to see M18-2 10
1 ABIN457348 FACS Func IHC (f) IHC (p) IP WB FITC Rat IgG2a Log in to see M18-2 9
1 ABIN457430 FACS Func IHC (f) IHC (p) IP WB Rat IgG2a Log in to see M18-2 8
1 ABIN1105952 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN669411 IF (p) IHC (p) WB Rabbit IgG AA 724-769 Log in to see Polyclonal 1
1 ABIN743723 IF (p) IHC (p) WB Rabbit IgG pSer756, pThr758, pThr759, AA 724-769 Log in to see Polyclonal 0
1 ABIN1714270 IF (p) IHC (p) WB Rabbit IgG AA 724-769, pSer756 Log in to see Polyclonal 0
1 ABIN1495137 IHC IHC (p) WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN2689033 BR IHC (f) IHC (fro) FACS IP WB Rat IgG2a, kappa Log in to see M18-2 7
1 ABIN4899848 FACS WB Goat IgG AA 24-702 Log in to see Polyclonal 0

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Epitope AA 720-769, pThr758
(46), (35), (28), (21), (16), (15), (11), (11), (7), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(504), (197), (118), (38), (24), (21), (8), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1)
Host Rabbit
(351), (173), (144), (9), (3), (1)
Clonality
Polyclonal
Conjugate This Integrin beta 2 antibody is un-conjugated
(81), (68), (48), (26), (12), (11), (8), (8), (7), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
(448), (286), (178), (172), (93), (88), (74), (73), (66), (40), (26), (15), (13), (11), (7), (6), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 antibody (ABIN1531540) Images
Specificity

CD18/ITGB2 (Phospho-Thr758) Antibody detects endogenous levels of CD18/ITGB2 only when phosphorylated at Thr758.

PhosphorylationH:T758 M:T760 R:T757

Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human CD18/ITGB2 around the phosphorylation site of Thr758.
Isotype IgG
Plasmids, Primers & others

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 antibody (ABIN1531540) Images back to top
Antigen
Alternative Name CD18/ITGB2 (ITGB2 Antibody Abstract)
Background

Synonyms: CD18, cell surface adhesion glycoproteins LFA- 1/CR3/p150,95 beta, complement receptor C3 beta, integrin beta-2, ITB2

NCBI Gene Symbol: ITGB2

Molecular Weight 84 kDa
Gene ID 3689
OMIM 116920
UniProt P05107
Pathways NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 antibody (ABIN1531540) Images back to top
Application Notes WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000
Comment

Unigene-Number: Hs.375957 (NCBI Gene Symbol: ITGB2)

Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 antibody (ABIN1531540) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20°C for at least 1 year.
Expiry Date 12 months

References for anti-Integrin beta 2 antibody (ABIN1531540)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Background publications

Hattori, Fujiyama, Taylor, Watanabe, Yada, Park, Toyoda, Ishii, Totoki, Choi, Groner, Soeda, Ohki, Takagi, Sakaki, Taudien, Blechschmidt, Polley, Menzel, Delabar, Kumpf, Lehmann, Patterson, Reichwald et al.: "The DNA sequence of human chromosome 21. ..." in: Nature, Vol. 405, Issue 6784, pp. 311-9, 2000

Kishimoto, OConnor, Lee, Roberts, Springer: "Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family." in: Cell, Vol. 48, Issue 4, pp. 681-90, 1987

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 antibody (ABIN1531540) back to top
Supplier Images
Western Blotting (WB) image for anti-Integrin beta 2 (ITGB2) (AA 720-769), (pThr758) antibody (ABIN1531540) Western blot analysis of extracts from COS7 cells treated with EGF 200ng/ml 30', usin...
Immunofluorescence (IF) image for anti-Integrin beta 2 (ITGB2) (AA 720-769), (pThr758) antibody (ABIN1531540) Immunofluorescence analysis of HepG2 cells, using CD18/ITGB2 (Phospho-Thr758) Antibod...