We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) Antibodies|
|Conjugate||This IRAK1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-IRAK1 AntibodyTarget Details IRAK1 Application Details Handling References for anti-IRAK1 antibody (ABIN360116) Images
|Specificity||This antibody reacts to IRAK.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human IRAK1.|
Target Details IRAK1Product Details anti-IRAK1 Antibody Application Details Handling References for anti-IRAK1 antibody (ABIN360116) Images back to top
|Alternative Name||IRAK1 (IRAK1 Antibody Abstract)|
|Background||Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.The tyrosine-like kinase (TLK) group consists of 40 tyrosine and serine-threonine kinases such as MLK (mixed-lineage kinase), LISK (LIMK/TESK), IRAK (interleukin-1 receptor-associated kinase), Raf, RIPK (receptor-interacting protein kinase), and STRK (activin and TGF-beta receptors) families.Synonyms: IRAK, IRAK-1, Interleukin-1 receptor-associated kinase 1|
|Gene ID||3654, 9606|
|Research Area||Cytokines, Receptors, Inflammation|
|Pathways||NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-IRAK1 Antibody Target Details IRAK1 Handling References for anti-IRAK1 antibody (ABIN360116) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IRAK1 Antibody Target Details IRAK1 Application Details References for anti-IRAK1 antibody (ABIN360116) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-IRAK1 antibody (ABIN360116)Product Details anti-IRAK1 Antibody Target Details IRAK1 Application Details Handling Images back to top
Jensen, Whitehead: "Pellino3, a novel member of the Pellino protein family, promotes activation of c-Jun and Elk-1 and may act as a scaffolding protein." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 3, pp. 1500-6, 2003
Jiang, Johnson, Nie, Qin, Bird, Li et al.: "Pellino 1 is required for interleukin-1 (IL-1)-mediated signaling through its interaction with the IL-1 receptor-associated kinase 4 (IRAK4)-IRAK-tumor necrosis factor receptor-associated factor 6 ..." in: The Journal of biological chemistry, Vol. 278, Issue 13, pp. 10952-6, 2003
Strelow, Kollewe, Wesche: "Characterization of Pellino2, a substrate of IRAK1 and IRAK4." in: FEBS letters, Vol. 547, Issue 1-3, pp. 157-61, 2003
Cao, Henzel, Gao: "IRAK: a kinase associated with the interleukin-1 receptor." in: Science (New York, N.Y.), Vol. 271, Issue 5252, pp. 1128-31, 1996
ImagesProduct Details anti-IRAK1 Antibody Target Details IRAK1 Application Details Handling References for anti-IRAK1 antibody (ABIN360116) back to top