anti-Human NFKBIA antibody for Immunofluorescence (fixed cells)

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • AI462015
  • Nfkbi
  • IKBA
  • MAD-3
  • NFKBI
  • ECI-6/IKBA
  • RL/IF-1
  • NFKB inhibitor alpha
  • nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha
  • NFKBIA
  • Nfkbia
Epitope
AA 2-317
80
48
42
41
26
23
20
20
19
18
15
15
10
10
7
7
6
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
544
293
238
52
40
29
19
15
2
2
2
1
1
Host
Mouse
382
170
7
Clonality
Monoclonal
Conjugate
This NFKBIA antibody is conjugated to FITC
19
14
14
12
11
9
8
6
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
3
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC)
441
223
208
97
93
91
74
40
39
19
17
6
5
4
4
3
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1999494
$ 198.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1999491 ELISA ELISA (Detection) FACS IF IF/ICC Mouse IgG2b AA 2-317 Log in to see 02 0
1 ABIN1844549 IF/ICC IHC WB Rabbit pSer32, pSer36 Log in to see Polyclonal 0
1 ABIN5928173 ELISA FACS IF/ICC IHC WB Mouse IgG1 AA 150-291 Log in to see 0
1 ABIN5928168 ELISA IF/ICC IHC WB Rabbit IgG pSer32, pSer36 Log in to see Polyclonal 0
1 ABIN5928165 ELISA IF/ICC IHC WB Rabbit IgG AA 30-34, AA 34-38 Log in to see Polyclonal 0
1 ABIN6106009 ELISA IF/ICC IHC WB Rabbit IgG pSer32, pSer36 Log in to see Polyclonal 0
1 ABIN6102835 ELISA IF/ICC IHC Rabbit IgG Tyr305 Log in to see Polyclonal 0
1 ABIN6105177 ELISA IF/ICC IHC WB Rabbit IgG Ser32, Ser36 Log in to see Polyclonal 0
1 ABIN5998335 FACS IF/ICC IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5998339 IF/ICC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5998337 IF/ICC IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5928162 ELISA FACS IF/ICC IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5928163 ELISA IF/ICC IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2934225 IF/ICC IHC WB Rabbit pSer32, pSer36 Log in to see Polyclonal 0
1 ABIN2994715 IF/ICC IHC WB Rabbit pSer32, pSer36 Log in to see Polyclonal 0
1 ABIN2993191 IF/ICC IHC WB Rabbit Log in to see Polyclonal 0
1 ABIN2993189 IF/ICC IHC IP WB Rabbit Log in to see Polyclonal 0

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Epitope AA 2-317
(80), (48), (42), (41), (26), (23), (20), (20), (19), (18), (15), (15), (10), (10), (7), (7), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(544), (293), (238), (52), (40), (29), (19), (15), (2), (2), (2), (1), (1)
Host Mouse
(382), (170), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NFKBIA antibody is conjugated to FITC
(19), (14), (14), (12), (11), (9), (8), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC)
(441), (223), (208), (97), (93), (91), (74), (40), (39), (19), (17), (6), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling Images
Specificity Human IkB alpha / NFKBIA
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IkB alpha / NFKBIA (rh IkB alpha / NFKBIA, ABIN2004994 NP_065390.1, Phe 2-Leu 317, and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Immunogen Recombinant Human IkB alpha / NFKBIA Protein (ABIN2004994)
Clone 02
Isotype IgG2b
Plasmids, Primers & others

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling Images back to top
Antigen
Alternative Name NFKBIA (NFKBIA Antibody Abstract)
Background NF-kappa-B inhibitor alpha, also known as Major histocompatibility complex enhancer- binding protein MAD3, I-kappa-B-alpha, NFKBIA and IKBA, is a cytoplasm and nucleus protein which belongs to theNF-kappa-B inhibitor family. NFKBIA contains five ANK repeats. NFKBIA inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, NFKBIA becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription. Defects in NFKBIA are the cause of ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant (ADEDAID). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ADEDAID is an ectodermal dysplasia associated with decreased production of pro-inflammatory cytokines and certain interferons, rendering patients susceptible to infection.
NCBI Accession NP_065390.1
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling Images back to top
Application Notes ICC/IF: 5-20 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Images back to top
Format Liquid
Concentration 0.1 mg/mL
Buffer Aqueous solution containing 0.5 % BSA and 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal
Storage 4 °C
Storage Comment This antibody is stable for 12 months from date of receipt when stored at 2°C-8°C. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Expiry Date 12 months

Images

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 2-317) antibody (FITC) (ABIN1999494) NFKBIA expression in HeLa and Jurkat cells. The cells were treated according to manuf...
Immunofluorescence (IF) image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 2-317) antibody (FITC) (ABIN1999494) Immunofluorescence staining of human NFKBIA in HeLa cells with mouse monoclonal antib...