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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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3.2286417 | ABIN2668625 | WB | Mouse | IgG1 | AA 32-291 | Log in to see | 6A920 | 6 | ||
3.2286417 | ABIN252664 | IP WB | Mouse | IgG1 kappa | pSer32, pSer36 | Log in to see | 39A1413 | 11 | ||
3.2286417 | ABIN2779315 | WB | Rabbit | N-Term | Log in to see | Polyclonal | 0 | |||
3.2286417 | ABIN674454 | IF (p) IHC (p) WB | Rabbit | IgG | AA 5-34 | Log in to see | Polyclonal | 12 | ||
3.2286417 | ABIN314166 | ICC IF IHC (p) IHC WB | Rabbit | pSer32, pSer36 | Log in to see | Polyclonal | 5 | |||
3.2286417 | ABIN2668610 | WB | Mouse | IgG1 | Log in to see | 39A1431 | 3 | |||
3.2286417 | ABIN2792665 | IHC WB | Rabbit | N-Term | Log in to see | Polyclonal | 1 | |||
3.2286417 | ABIN2856763 | ICC IF IHC (p) WB | Rabbit | IgG | Center | Log in to see | Polyclonal | 0 | ||
3.2286417 | ABIN2792229 | WB | Rabbit | Middle Region | Log in to see | Polyclonal | 1 | |||
3.2286417 | ABIN2792116 | WB | Rabbit | N-Term | Log in to see | Polyclonal | 1 | |||
3.2286417 | ABIN2707587 | IC IF IHC WB | Rabbit | N-Term | Log in to see | Polyclonal | 0 | |||
3.2286417 | ABIN2707588 | IC IF IP IHC WB | Rabbit | N-Term | Log in to see | Polyclonal | 0 | |||
3.2286417 | ABIN2957632 | IHC WB | Rabbit | N-Term, pTyr42 | Log in to see | Polyclonal | 0 | |||
3.2286417 | ABIN2707586 | IC IF IHC WB | Rabbit | N-Term, pSer32, pSer36 | Log in to see | Polyclonal | 0 | |||
3.2286417 | ABIN968113 | IF IP IHC WB | Mouse | IgG1 | AA 145-302 | Log in to see | 25-IkBa-MAD | 5 | ||
3.2286417 | ABIN1531880 | IF IHC ELISA WB | Rabbit | IgG | AA 15-64, pSer32 | Log in to see | Polyclonal | 1 | ||
3.2286417 | ABIN1531661 | IHC ELISA WB | Rabbit | IgG | AA 268-317, pTyr305 | Log in to see | Polyclonal | 1 | ||
3.2286417 | ABIN3043889 | IHC (p) WB | Rabbit | IgG | AA 3-112 | Log in to see | Polyclonal | 0 | ||
3.2286417 | ABIN1532911 | IHC ELISA WB | Rabbit | IgG | AA 15-64 | Log in to see | Polyclonal | 0 | ||
3.2286417 | ABIN1532903 | IHC ELISA WB | Rabbit | IgG | AA 9-58 | Log in to see | Polyclonal | 0 |
General |
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Antigen | Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies |
Epitope | AA 32-291 Alternatives |
Reactivity | Human, Mouse (Murine), Rat (Rattus) Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This NFKBIA antibody is un-conjugated Alternatives |
Application |
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
Alternatives
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33 references available |
Supplier | Log in to see |
Product Details anti-NFKBIA AntibodyTarget Details NFKBIA Application Details Handling References for anti-NFKBIA antibody (ABIN252471) Images |
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Specificity | A 40 kDa band is observed. |
Purification | Protein G purified |
Immunogen | A recombinant protein corresponding to amino acid residues 32-291 of human IkBa was used as immunogen. |
Clone | 6A920 |
Isotype | IgG1 kappa |
Target Details NFKBIAProduct Details anti-NFKBIA Antibody Application Details Handling References for anti-NFKBIA antibody (ABIN252471) Images back to top |
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Antigen | |
Alternative Name | IkB-alpha (NFKBIA Antibody Abstract) |
Background | Gene Symbol: NFKBIA |
Gene ID | 4792 |
UniProt | P25963 |
Pathways | NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades |
Application DetailsProduct Details anti-NFKBIA Antibody Target Details NFKBIA Handling References for anti-NFKBIA antibody (ABIN252471) Images back to top |
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Application Notes | Western Blot 1-2 μg/mL, Simple Western 1:20, Flow Cytometry 0.25-1 μg/10^6 cells, Immunohistochemistry 1:20-1:1000, Immunocytochemistry/Immunofluorescence 1:20-1:1000, Immunoprecipitation 1 μg/mL, Immunohistochemistry-Paraffin 1:100, CyTOF-readyIn Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size. |
Comment |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
Restrictions | For Research Use only |
HandlingProduct Details anti-NFKBIA Antibody Target Details NFKBIA Application Details References for anti-NFKBIA antibody (ABIN252471) Images back to top |
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Format | Liquid |
Concentration | 1.0 mg/mL |
Buffer |
PBS Buffer contains: 0.05 % Sodium Azide |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Avoid freeze-thaw cycles |
Storage | 4 °C,-20 °C |
Storage Comment | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
References for anti-NFKBIA antibody (ABIN252471)Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling Images back to top |
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Product cited in: |
Zhu, Willette-Brown, Song, Lomada, Song, Xue, Gray, Zhao, Davis, Sun, Zhang, Wu, Zhan, Richie, Hu: "Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis." in: Cell host & microbe, Vol. 21, Issue 4, pp. 478-493.e7, 2017 Gao, Liu, Wu, Zhao, Wang, Gao, Wen, Zhang, Guo, Zhou, Yuan: "IL-17 intensifies IFN-γ-induced NOS2 upregulation in RAW 264.7 cells by further activating STAT1 and NF-κB." in: International journal of molecular medicine, Vol. 37, Issue 2, pp. 347-58, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting Tomasi, Ramani, Ryoo: "Ubiquitin-Conjugating Enzyme 9 Phosphorylation as a Novel Mechanism for Potentiation of the Inflammatory Response." in: The American journal of pathology, Vol. 186, Issue 9, pp. 2326-36, 2016 Further details: Western Blotting Hochrainer, Pejanovic, Olaseun, Zhang, Iadecola, Anrather: "The ubiquitin ligase HERC3 attenuates NF-κB-dependent transcription independently of its enzymatic activity by delivering the RelA subunit for degradation." in: Nucleic acids research, Vol. 43, Issue 20, pp. 9889-904, 2015 (Sample species: Human). Further details: Western Blotting Ikeno, Apel, Zouboulis, Luger, Böhm: "L-Ascorbyl-2-phosphate attenuates NF-?B signaling in SZ95 sebocytes without affecting IL-6 and IL-8 secretion." in: Archives of dermatological research, Vol. 307, Issue 7, pp. 595-605, 2015 (Sample species: Human). Further details: Western Blotting Tabit, Shenouda, Holbrook, Fetterman, Kiani, Frame, Kluge, Held, Dohadwala, Gokce, Farb, Rosenzweig, Ruderman, Vita, Hamburg: "Protein kinase C-β contributes to impaired endothelial insulin signaling in humans with diabetes mellitus." in: Circulation, Vol. 127, Issue 1, pp. 86-95, 2013 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence Yang, Zhao, Liu, Hu, Sun, Chang, Wei, Fan, Han: "Inhibition of NADPH Oxidase Mediates Protective Effect of Cardiotonic Pills against Rat Heart Ischemia/Reperfusion Injury." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2013, pp. 728020, 2013 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections) Delhase, Kim, Lee, Naiki-Ito, Chen, Ahn, Murata, Kim, Lautsch, Kobayashi, Shirai, Karin, Nakanishi: "TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 4, pp. E177-86, 2012 Pejanovic, Hochrainer, Liu, Aerne, Soares, Anrather: "Regulation of nuclear factor κB (NF-κB) transcriptional activity via p65 acetylation by the chaperonin containing TCP1 (CCT)." in: PLoS ONE, Vol. 7, Issue 7, pp. e42020, 2012 Harikumar, Sung, Pandey, Guha, Krishnan, Aggarwal: "Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway." in: Molecular pharmacology, Vol. 77, Issue 5, pp. 818-27, 2010 Yu, Su, Zou, Liu, Wu, Kong, Zhuang, Sun, Liu, Hu, Li, Strominger, Zang, Pei, Ge: "An essential function for beta-arrestin 2 in the inhibitory signaling of natural killer cells." in: Nature immunology, Vol. 9, Issue 8, pp. 898-907, 2008 (Sample species: Mouse (Murine)). Nishioka, Ikezoe, Jing, Umezawa, Yokoyama et al.: "DHMEQ, a novel nuclear factor-kappaB inhibitor, induces selective depletion of alloreactive or phytohaemagglutinin-stimulated peripheral blood mononuclear cells, decreases production of T helper type ..." in: Immunology, Vol. 124, Issue 2, pp. 198-205, 2008 Shakibaei, John, Schulze-Tanzil, Lehmann, Mobasheri et al.: "Suppression of NF-kappaB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes: Implications for the treatment ..." in: Biochemical pharmacology, Vol. 73, Issue 9, pp. 1434-45, 2007 Endo, Nishio, Enzler, Cottam, Fukuda, James, Karin, Kipps: "BAFF and APRIL support chronic lymphocytic leukemia B-cell survival through activation of the canonical NF-kappaB pathway." in: Blood, Vol. 109, Issue 2, pp. 703-10, 2007 (Sample species: Human). Further details: Western Blotting Vilimas, Mascarenhas, Palomero, Mandal, Buonamici, Meng, Thompson, Spaulding, Macaroun, Alegre, Kee, Ferrando, Miele, Aifantis: "Targeting the NF-kappaB signaling pathway in Notch1-induced T-cell leukemia." in: Nature medicine, Vol. 13, Issue 1, pp. 70-7, 2007 Further details: Western Blotting,Immunoprecipitation Perez-Moreno, Davis, Wong, Pasolli, Reynolds, Fuchs: "p120-catenin mediates inflammatory responses in the skin." in: Cell, Vol. 124, Issue 3, pp. 631-44, 2006 (Sample species: Mouse (Murine)). Further details: Western Blotting Chang, Kamata, Solinas, Luo, Maeda, Venuprasad, Liu, Karin: "The E3 ubiquitin ligase itch couples JNK activation to TNFalpha-induced cell death by inducing c-FLIP(L) turnover." in: Cell, Vol. 124, Issue 3, pp. 601-13, 2006 Further details: Western Blotting Strozyk, Pöppelmann, Schwarz, Kulms: "Differential effects of NF-kappaB on apoptosis induced by DNA-damaging agents: the type of DNA damage determines the final outcome." in: Oncogene, Vol. 25, Issue 47, pp. 6239-51, 2006 (Sample species: Human). Further details: Western Blotting Macagno, Molteni, Rinaldi, Bertoni, Lanzavecchia, Rossetti, Sallusto: "A cyanobacterial LPS antagonist prevents endotoxin shock and blocks sustained TLR4 stimulation required for cytokine expression." in: The Journal of experimental medicine, Vol. 203, Issue 6, pp. 1481-92, 2006 Further details: Western Blotting Santhi, Sebastian, Varghese, Prakash, Pillai: "NF-kappaB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins." in: Experimental and molecular pathology, Vol. 81, Issue 2, pp. 123-30, 2006 |
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