anti-Rat (Rattus) NFKBIA antibody for Western Blotting

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • IKBA
  • MAD-3
  • NFKBI
  • AI462015
  • Nfkbi
  • RL/IF-1
  • ECI-6/IKBA
  • ikbaA
  • wu:fj16a07
  • zgc:85769
  • NFKB inhibitor alpha
  • nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha a
  • NFKBIA
  • Nfkbia
  • nfkbiaa
Epitope
AA 32-291
77
45
42
41
27
22
20
19
17
15
15
11
11
7
7
6
5
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
488
293
237
49
37
26
19
15
2
2
2
1
1
Host
Mouse
386
111
7
Clonality
Monoclonal
Conjugate
This NFKBIA antibody is un-conjugated
16
13
11
8
7
6
6
5
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
392
192
180
100
87
70
42
42
41
19
19
6
5
4
3
3
2
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN252471
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.080771 ABIN252664 IP WB Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413 11
3.080771 ABIN674454 IF (p) IHC (p) WB Rabbit IgG AA 5-34 Log in to see Polyclonal 12
3.080771 ABIN3043889 IHC (p) WB Rabbit IgG AA 3-112 Log in to see Polyclonal 0
3.080771 ABIN2792229 WB Rabbit Middle Region Log in to see Polyclonal 1
3.080771 ABIN2792116 WB Rabbit N-Term Log in to see Polyclonal 1
3.080771 ABIN2707586 IC IF IHC WB Rabbit N-Term, pSer32, pSer36 Log in to see Polyclonal 0
3.080771 ABIN2957632 IHC WB Rabbit N-Term, pTyr42 Log in to see Polyclonal 0
3.080771 ABIN3185247 ELISA WB Rabbit IgG N-Term Log in to see Polyclonal 0
3.080771 ABIN1531661 IHC ELISA WB Rabbit IgG AA 268-317, pTyr305 Log in to see Polyclonal 1
3.080771 ABIN1531880 IF IHC ELISA WB Rabbit IgG AA 15-64, pSer32 Log in to see Polyclonal 1
3.080771 ABIN1532903 IHC ELISA WB Rabbit IgG AA 9-58 Log in to see Polyclonal 0
3.080771 ABIN1532911 IHC ELISA WB Rabbit IgG AA 15-64 Log in to see Polyclonal 0
3.080771 ABIN3185250 ELISA IF IHC WB Rabbit IgG Thr17 Log in to see Polyclonal 0
3.080771 ABIN3182050 ELISA IF IHC WB Rabbit IgG pSer32, pSer36 Log in to see Polyclonal 0
3.080771 ABIN1873901 IF IP IHC WB Rabbit IgG Log in to see Polyclonal 0
3.080771 ABIN6262504 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 1
3.080771 ABIN3188043 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.080771 ABIN252071 ELISA WB Biotin Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413 1
3.080771 ABIN5952450 ICC IF IHC IHC (p) IP WB Rabbit IgG Log in to see SZ00-07 0
3.080771 ABIN5961651 ELISA IF IHC (p) WB Rabbit IgG Tyr42 Log in to see Polyclonal 0

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Epitope AA 32-291
(77), (45), (42), (41), (27), (22), (20), (19), (17), (15), (15), (11), (11), (7), (7), (6), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(488), (293), (237), (49), (37), (26), (19), (15), (2), (2), (2), (1), (1)
Host Mouse
(386), (111), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NFKBIA antibody is un-conjugated
(16), (13), (11), (8), (7), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
(392), (192), (180), (100), (87), (70), (42), (42), (41), (19), (19), (6), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Pubmed 33 references available
Supplier Log in to see

Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling References for anti-NFKBIA antibody (ABIN252471) Images
Specificity A 40 kDa band is observed.
Purification Protein G purified
Immunogen A recombinant protein corresponding to amino acid residues 32-291 of human IkBa was used as immunogen.
Clone 6A920
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling References for anti-NFKBIA antibody (ABIN252471) Images back to top
Antigen
Alternative Name IkB-alpha (NFKBIA Antibody Abstract)
Background Gene Symbol: NFKBIA
Gene ID 4792
UniProt P25963
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling References for anti-NFKBIA antibody (ABIN252471) Images back to top
Application Notes Western Blot 1-2 μg/mL, Simple Western 1:20, Flow Cytometry 0.25-1 μg/10^6 cells, Immunohistochemistry 1:20-1:1000, Immunocytochemistry/Immunofluorescence 1:20-1:1000, Immunoprecipitation 1 μg/mL, Immunohistochemistry-Paraffin 1:100, CyTOF-readyIn Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details References for anti-NFKBIA antibody (ABIN252471) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-NFKBIA antibody (ABIN252471)

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling Images back to top
Product cited in:

Zhu, Willette-Brown, Song, Lomada, Song, Xue, Gray, Zhao, Davis, Sun, Zhang, Wu, Zhan, Richie, Hu: "Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis." in: Cell host & microbe, Vol. 21, Issue 4, pp. 478-493.e7, 2017

Gao, Liu, Wu, Zhao, Wang, Gao, Wen, Zhang, Guo, Zhou, Yuan: "IL-17 intensifies IFN-γ-induced NOS2 upregulation in RAW 264.7 cells by further activating STAT1 and NF-κB." in: International journal of molecular medicine, Vol. 37, Issue 2, pp. 347-58, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Tomasi, Ramani, Ryoo: "Ubiquitin-Conjugating Enzyme 9 Phosphorylation as a Novel Mechanism for Potentiation of the Inflammatory Response." in: The American journal of pathology, Vol. 186, Issue 9, pp. 2326-36, 2016 Further details: Western Blotting

Hochrainer, Pejanovic, Olaseun, Zhang, Iadecola, Anrather: "The ubiquitin ligase HERC3 attenuates NF-κB-dependent transcription independently of its enzymatic activity by delivering the RelA subunit for degradation." in: Nucleic acids research, Vol. 43, Issue 20, pp. 9889-904, 2015 (Sample species: Human). Further details: Western Blotting

Ikeno, Apel, Zouboulis, Luger, Böhm: "L-Ascorbyl-2-phosphate attenuates NF-?B signaling in SZ95 sebocytes without affecting IL-6 and IL-8 secretion." in: Archives of dermatological research, Vol. 307, Issue 7, pp. 595-605, 2015 (Sample species: Human). Further details: Western Blotting

Tabit, Shenouda, Holbrook, Fetterman, Kiani, Frame, Kluge, Held, Dohadwala, Gokce, Farb, Rosenzweig, Ruderman, Vita, Hamburg: "Protein kinase C-β contributes to impaired endothelial insulin signaling in humans with diabetes mellitus." in: Circulation, Vol. 127, Issue 1, pp. 86-95, 2013 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Yang, Zhao, Liu, Hu, Sun, Chang, Wei, Fan, Han: "Inhibition of NADPH Oxidase Mediates Protective Effect of Cardiotonic Pills against Rat Heart Ischemia/Reperfusion Injury." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2013, pp. 728020, 2013 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Delhase, Kim, Lee, Naiki-Ito, Chen, Ahn, Murata, Kim, Lautsch, Kobayashi, Shirai, Karin, Nakanishi: "TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 4, pp. E177-86, 2012

Pejanovic, Hochrainer, Liu, Aerne, Soares, Anrather: "Regulation of nuclear factor κB (NF-κB) transcriptional activity via p65 acetylation by the chaperonin containing TCP1 (CCT)." in: PLoS ONE, Vol. 7, Issue 7, pp. e42020, 2012

Harikumar, Sung, Pandey, Guha, Krishnan, Aggarwal: "Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway." in: Molecular pharmacology, Vol. 77, Issue 5, pp. 818-27, 2010

Yu, Su, Zou, Liu, Wu, Kong, Zhuang, Sun, Liu, Hu, Li, Strominger, Zang, Pei, Ge: "An essential function for beta-arrestin 2 in the inhibitory signaling of natural killer cells." in: Nature immunology, Vol. 9, Issue 8, pp. 898-907, 2008 (Sample species: Mouse (Murine)).

Nishioka, Ikezoe, Jing, Umezawa, Yokoyama et al.: "DHMEQ, a novel nuclear factor-kappaB inhibitor, induces selective depletion of alloreactive or phytohaemagglutinin-stimulated peripheral blood mononuclear cells, decreases production of T helper type ..." in: Immunology, Vol. 124, Issue 2, pp. 198-205, 2008

Shakibaei, John, Schulze-Tanzil, Lehmann, Mobasheri et al.: "Suppression of NF-kappaB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes: Implications for the treatment ..." in: Biochemical pharmacology, Vol. 73, Issue 9, pp. 1434-45, 2007

Endo, Nishio, Enzler, Cottam, Fukuda, James, Karin, Kipps: "BAFF and APRIL support chronic lymphocytic leukemia B-cell survival through activation of the canonical NF-kappaB pathway." in: Blood, Vol. 109, Issue 2, pp. 703-10, 2007 (Sample species: Human). Further details: Western Blotting

Vilimas, Mascarenhas, Palomero, Mandal, Buonamici, Meng, Thompson, Spaulding, Macaroun, Alegre, Kee, Ferrando, Miele, Aifantis: "Targeting the NF-kappaB signaling pathway in Notch1-induced T-cell leukemia." in: Nature medicine, Vol. 13, Issue 1, pp. 70-7, 2007 Further details: Western Blotting,Immunoprecipitation

Perez-Moreno, Davis, Wong, Pasolli, Reynolds, Fuchs: "p120-catenin mediates inflammatory responses in the skin." in: Cell, Vol. 124, Issue 3, pp. 631-44, 2006 (Sample species: Mouse (Murine)). Further details: Western Blotting

Chang, Kamata, Solinas, Luo, Maeda, Venuprasad, Liu, Karin: "The E3 ubiquitin ligase itch couples JNK activation to TNFalpha-induced cell death by inducing c-FLIP(L) turnover." in: Cell, Vol. 124, Issue 3, pp. 601-13, 2006 Further details: Western Blotting

Strozyk, Pöppelmann, Schwarz, Kulms: "Differential effects of NF-kappaB on apoptosis induced by DNA-damaging agents: the type of DNA damage determines the final outcome." in: Oncogene, Vol. 25, Issue 47, pp. 6239-51, 2006 (Sample species: Human). Further details: Western Blotting

Macagno, Molteni, Rinaldi, Bertoni, Lanzavecchia, Rossetti, Sallusto: "A cyanobacterial LPS antagonist prevents endotoxin shock and blocks sustained TLR4 stimulation required for cytokine expression." in: The Journal of experimental medicine, Vol. 203, Issue 6, pp. 1481-92, 2006 Further details: Western Blotting

Santhi, Sebastian, Varghese, Prakash, Pillai: "NF-kappaB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins." in: Experimental and molecular pathology, Vol. 81, Issue 2, pp. 123-30, 2006

Images

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling References for anti-NFKBIA antibody (ABIN252471) back to top
Supplier Images
Western Blotting (WB) image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 32-291) antibody (ABIN252471) Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucina...
Western Blotting (WB) image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 32-291) antibody (ABIN252471) Western blot analysis of IkBa using ABIN252471 at 2 ug/ml in (A) Daudi and (B) NIH 3T...
Western Blotting (WB) image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 32-291) antibody (ABIN252471) Western Blot: IkB-alpha Antibody (6A920) [ABIN2524717] - Jurkat cells were treated fo...
Simple Western (SimWes) image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 32-291) antibody (ABIN252471) Simple Western: IkB-alpha Antibody (6A920) [ABIN2524717] - Simple Western lane view s...
Flow Cytometry (FACS) image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 32-291) antibody (ABIN252471) Flow Cytometry: IkB-alpha Antibody (6A920) [ABIN2524717] - Intracellular staining of ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 32-291) antibody (ABIN252471) Immunohistochemistry-Paraffin: IkB-alpha Antibody (6A920) [ABIN2524717] - Formalin-fi...
 image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 32-291) antibody (ABIN252471) Knockout Validated: IkB-alpha Antibody (6A920) - Western blot shows lysates of HEK29...