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General |
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Antigen | Nerve Growth Factor Receptor (NGFR) Antibodies |
Reactivity | Cat (Feline), Human, Monkey, Rabbit Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This NGFR antibody is un-conjugated Alternatives |
Application |
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Alternatives
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Supplier | Log in to see |
Product Details anti-NGFR AntibodyTarget Details NGFR Application Details Handling Images |
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Specificity | Recognizes p75NGFR. Species Reactivity: Reacts with human, monkey (Cebus apella, Macaca fascicularis, mulatta, and nemestrina), baboon (Papio anubis and Cynocephalus), cat, rabbit and ferret. Does NOT react with rat or mouse. Cellular Location: Cell surface and cytoplasmic.Positive Control: Melanoma. |
Immunogen | NGFR from A875 melanoma cells. |
Clone | NGFR5 |
Isotype | IgG1 |
Target Details NGFRProduct Details anti-NGFR Antibody Application Details Handling Images back to top |
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Antigen | |
Alternative Name | CD271 / NGFR (NGFR Antibody Abstract) |
Background | Nerve Growth Factor (NGF) is important for the development, differentiation, and survival of a variety of neuronal and non-neuronal cells. The action of NGF is mediated by binding two distinct receptors, high affinity p140 and low affinity p75. This antibody recognizes a glycoprotein of 75 kDa identified as low affinity Nerve Growth Factor Receptor (p75NGFR), also called Neurotrophin Receptor (p75NTR). |
Pathways | NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding |
Application DetailsProduct Details anti-NGFR Antibody Target Details NGFR Handling Images back to top |
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Application Notes | Flow cytometry Functional: Inhibits binding of NGF to Melanoma cells (remove sodium azide). Immunofluorescence Immunohistochemistry: NOT SUITABLE. Western blot: NOT SUITABLE. The optimal dilution for a specific application should be determined by the researcher. |
Restrictions | For Research Use only |
HandlingProduct Details anti-NGFR Antibody Target Details NGFR Application Details Images back to top |
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Format | Liquid |
Buffer | 500 µL of 200 µg/mL monoclonal antibody in 10 mM PBS, pH 7.4 with protein stabilizer and 0.09% sodium azide. Purified from ascites fluid by Protein G chromatography. |
Preservative | Sodium azide |
Precaution of Use | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Storage | 4 °C |