anti-Human p65 antibody for Immunohistochemistry (Frozen Sections)

Recommended p65 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor-KB P65 (NFkBP65) Antibodies
  • C-Rel
  • NFKB3
  • p65
  • NFkB
  • c-rel
  • zgc:100833
  • REL proto-oncogene, NF-kB subunit
  • RELA proto-oncogene, NF-kB subunit
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel avian reticuloendotheliosis viral oncogene homolog
  • REL
  • RELA
  • Rela
  • Rel
  • rel
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1161
702
565
71
66
53
24
20
15
12
8
6
6
4
4
2
1
1
Host
Mouse
1040
124
11
10
1
Clonality
Monoclonal
Conjugate
This p65 antibody is un-conjugated
28
28
21
18
16
14
13
11
10
10
10
8
8
8
8
8
8
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
912
524
403
237
192
191
106
86
62
37
30
20
19
13
12
6
5
5
5
4
4
2
1
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Catalog No. ABIN126921
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN233530 ELISA GS IHC IHC (fro) IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN209098 ELISA FACS GS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG3 Log in to see 0
1 ABIN121887 EMSA EIA IHC (fro) IP WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN187261 IHC (fro) IP WB Mouse IgG1 Log in to see BV706 0
1 ABIN187876 IHC (fro) WB Rabbit Log in to see Polyclonal 0

General

Antigen Nuclear Factor-KB P65 (NFkBP65) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1161), (702), (565), (71), (66), (53), (24), (20), (15), (12), (8), (6), (6), (4), (4), (2), (1), (1)
Host Mouse
(1040), (124), (11), (10), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p65 antibody is un-conjugated
(28), (28), (21), (18), (16), (14), (13), (11), (10), (10), (10), (8), (8), (8), (8), (8), (8), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(912), (524), (403), (237), (192), (191), (106), (86), (62), (37), (30), (20), (19), (13), (12), (6), (5), (5), (5), (4), (4), (2), (1)
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Product Details anti-p65 Antibody

Target Details p65 Application Details Handling Images
Specificity This antibody recognizes NFkB.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse and Rat.
Characteristics Synonyms: NF kappa B p65, NFkB p65, Transcription factor p65, Rel A, NFKB3, Nuclear factorNF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer inB-cells 3
Purification Affinity Chromatography.
Immunogen Synthetic peptide from Human NFkB. Remarks: Molecular Weight: 65 kDa
Clone S8 (2A12A7)
Isotype IgG1
Plasmids, Primers & others

Target Details p65

Product Details anti-p65 Antibody Application Details Handling Images back to top
Antigen
Alternative Name RELA / NF-kB p65 (NFkBP65 Antibody Abstract)
Background NFkB was identified as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), proinflammatory cytokines, and viral infection. IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.Synonyms: NF kappa B p65, NFKB3, NFkB p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Rel A, Transcription factor p65
Gene ID 5970
UniProt Q04206
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-p65 Antibody Target Details p65 Handling Images back to top
Application Notes Western Blot: 1-4 μg/mL. Immunoprecipitation. Immunohistochemistry. A431 cells can be used as a Positive Control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-p65 Antibody Target Details p65 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer PBS containing 1 mg/mL BSA, 1.5 mM Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C/-80 °C
Storage Comment Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-p65 Antibody Target Details p65 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Nuclear Factor-KB P65 (NFkBP65) antibody (ABIN126921) anti-Nuclear Factor-KB P65 (NFkBP65) antibody