anti-Rat (Rattus) p65 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended p65 Antibody

Antigen
Nuclear Factor-KB P65 (NFkBP65) Antibodies
  • C-Rel
  • NFKB3
  • p65
  • NFkB
  • c-rel
  • zgc:100833
  • REL proto-oncogene, NF-kB subunit
  • RELA proto-oncogene, NF-kB subunit
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel avian reticuloendotheliosis viral oncogene homolog
  • REL
  • RELA
  • Rela
  • Rel
  • rel
Epitope
AA 50-100
80
80
78
62
59
51
50
48
38
32
25
24
21
17
16
16
16
16
15
15
13
13
12
11
10
10
9
8
8
8
6
6
6
6
6
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
1213
749
605
72
70
51
22
20
16
12
10
8
6
4
1
1
1
Host
Rabbit
1143
82
11
2
1
Clonality
Polyclonal
Conjugate
This p65 antibody is un-conjugated
45
28
26
26
26
26
26
26
20
13
12
11
8
8
8
8
8
3
2
2
Application
Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
875
512
387
190
185
178
105
43
39
36
26
20
19
13
12
7
5
4
3
1
Options

Available images

Catalog No. ABIN668961
$ 329.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
34 ABIN3044253 ICC IHC (p) WB Rabbit IgG AA 143-158, Middle Region Polyclonal 50
31.477398 ABIN3188030 ELISA IHC (p) WB Rabbit IgG acLys314, acLys315 Polyclonal 0
29.5 ABIN730528 IF (p) IHC (p) WB Rabbit IgG pSer536 Polyclonal 11
23.173733 ABIN252655 CyTOF FACS IHC IHC (p) SimWes WB Mouse IgG1 kappa 112A1021 11
22.477398 ABIN3187852 ELISA IHC (p) WB Rabbit IgG Thr75 Polyclonal 0
22.477398 ABIN3181891 ELISA IHC (p) WB Rabbit IgG acLys218 Polyclonal 0
19.477398 ABIN3187851 ELISA IHC (p) WB Rabbit IgG Thr743 Polyclonal 0
19 ABIN5693244 ELISA IHC (p) WB Rabbit AA 99-551 Polyclonal 45
16 ABIN6654172 ELISA IHC (p) WB Rabbit IgG AA 99-551 Polyclonal 0
14.859131 ABIN218583 ELISA GS IF IHC IHC (p) IP WB Rabbit C-Term Polyclonal 0
14.5 ABIN700555 IF (p) IHC (p) WB Rabbit IgG AA 250-300, pSer276 Polyclonal 2
14.173733 ABIN4349852 ICC IF IHC IHC (p) WB Rabbit pSer536 Polyclonal 4
14.173733 ABIN257452 ICC IF IHC (p) IHC WB Rabbit pSer276 Polyclonal 2
11.859131 ABIN498094 IHC (p) IP WB Rabbit Polyclonal 0
10 ABIN6652275 IF IHC (p) WB Rabbit pSer529 Polyclonal 4
9.314602 ABIN151596 ICC IF IHC IHC (p) IP WB Rabbit C-Term Polyclonal 29
8.859131 ABIN498856 IHC (p) Rabbit pSer536 Polyclonal 0
8.859131 ABIN498944 IHC (p) Rabbit Polyclonal 0
8.5 ABIN5961224 ELISA IF IHC (p) WB Rabbit IgG pSer536 Polyclonal 1
8.173733 ABIN4349897 CyTOF FACS IHC (p) WB Mouse IgG1 kappa 112A1021 4

General

Antigen Nuclear Factor-KB P65 (NFkBP65) Antibodies
Epitope AA 50-100
(80), (80), (78), (62), (59), (51), (50), (48), (38), (32), (25), (24), (21), (17), (16), (16), (16), (16), (15), (15), (13), (13), (12), (11), (10), (10), (9), (8), (8), (8), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(1213), (749), (605), (72), (70), (51), (22), (20), (16), (12), (10), (8), (6), (4), (1), (1), (1)
Host Rabbit
(1143), (82), (11), (2), (1)
Clonality
Polyclonal
Conjugate This p65 antibody is un-conjugated
(45), (28), (26), (26), (26), (26), (26), (26), (20), (13), (12), (11), (8), (8), (8), (8), (8), (3), (2), (2)
Application Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(875), (512), (387), (190), (185), (178), (105), (43), (39), (36), (26), (20), (19), (13), (12), (7), (5), (4), (3), (1)
Pubmed 29 references available
Supplier

Product Details anti-p65 Antibody

Target Details p65 Application Details Handling References for anti-p65 antibody (ABIN668961) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human NFKBp65
Isotype IgG
Plasmids, Primers & others

Target Details p65

Product Details anti-p65 Antibody Application Details Handling References for anti-p65 antibody (ABIN668961) Images back to top
Antigen
Alternative Name NFKB p65 (NFkBP65 Antibody Abstract)
Background

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p15, NFKB1/p5, REL and NFKB2/p52 and the heterodimeric p65-p5 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p5 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

Subcellular location: Cytoplasm

Synonyms: p65, NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA

Gene ID 5970
UniProt Q04206
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-p65 Antibody Target Details p65 Handling References for anti-p65 antibody (ABIN668961) Images back to top
Application Notes WB 1:100-1000
FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-p65 Antibody Target Details p65 Application Details References for anti-p65 antibody (ABIN668961) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-p65 antibody (ABIN668961)

Product Details anti-p65 Antibody Target Details p65 Application Details Handling Images back to top
Product cited in:

Oyagbemi, Omobowale, Ola-Davies, Asenuga, Ajibade, Adejumobi, Afolabi, Ogunpolu, Falayi, Ayodeji, Hassan, Saba, Adedapo, Yakubu: "Ameliorative effect of Rutin on sodium fluoride-induced hypertension through modulation of Kim-1/NF-κB/Nrf2 signaling pathway in rats." in: Environmental toxicology, Vol. 33, Issue 12, pp. 1284-1297, 2019 Method employed by authors: Immunohistochemistry (IHC), Immunohistochemistry (IHC)

Xu, Liu, Yu, Wu, Lu: "Effect of recombinant Trichinella spiralis cysteine proteinase inhibitor on TNBS-induced experimental inflammatory bowel disease in mice." in: International immunopharmacology, Vol. 66, pp. 28-40, 2019 Method employed by authors: Western Blotting (WB), Western Blotting (WB)

Fattori, Borghi, Guazelli, Giroldo, Crespigio, Bussmann, Coelho-Silva, Ludwig, Mazzuco, Casagrande, Verri: "Vinpocetine reduces diclofenac-induced acute kidney injury through inhibition of oxidative stress, apoptosis, cytokine production, and NF-κB activation in mice." in: Pharmacological research, Vol. 120, pp. 10-22, 2018 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)). Further details: Sample: kidney

Sun, Nemoto, Hong, Sasaki: "Modulation of stromal cell-derived factor 1 alpha (SDF-1α) and its receptor CXCR4 in Porphyromonas gingivalis-induced periodontal inflammation." in: BMC oral health, Vol. 17, Issue 1, pp. 26, 2018 Method employed by authors: Immunohistochemistry (IHC), Immunohistochemistry (IHC)

Xi, Wang, Chen, Yang, Hu, Zhang, Weng, Xu: "Expressions of IL-6, TNF-α and NF-κB in the skin of Chinese brown frog (Rana dybowskii)." in: European journal of histochemistry : EJH, Vol. 61, Issue 4, pp. 2834, 2018 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Lin, Geng, Zhao, Lin, Zhu, Tian: "MiR-21 Regulates TNF-α-Induced CD40 Expression via the SIRT1-NF-κB Pathway in Renal Inner Medullary Collecting Duct Cells." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 41, Issue 1, pp. 124-136, 2017 Method employed by authors: Western Blotting (WB), Western Blotting (WB)

Ganguly, Wen, Myer, Czech, Sahu, Steinmetz, Raman: "Anti-atherogenic effect of trivalent chromium-loaded CPMV nanoparticles in human aortic smooth muscle cells under hyperglycemic conditions in vitro." in: Nanoscale, Vol. 8, Issue 12, pp. 6542-54, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Yang, Zhong, Li, Xiong, Wang, Peng, Ye: "Hypothermic machine perfusion increases A20 expression which protects renal cells against ischemia/reperfusion injury by suppressing inflammation, apoptosis and necroptosis." in: International journal of molecular medicine, Vol. 38, Issue 1, pp. 161-71, 2016 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Rabbit). Further details: Sample: kidney

Oyagbemi, Omobowale, Asenuga, Adejumobi, Ajibade, Ige, Ogunpolu, Adedapo, Yakubu: "Sodium fluoride induces hypertension and cardiac complications through generation of reactive oxygen species and activation of nuclear factor kappa beta." in: Environmental toxicology, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rat (Rattus)). Further details: Sample: heart, kidney

Xi, Hu, Guo, Wang, Sheng, Han, Yuan, Weng, Xu: "Immunoreactivities of NF-κB, IL-1β and IL-1R in the skin of Chinese brown frog (Rana dybowskii)." in: Acta histochemica, Vol. 119, Issue 1, pp. 64-70, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Others). Further details: Sample: skin

Zheng, Liu, Li, Tang, Zhang, Tang: "Lithium posttreatment confers neuroprotection through glycogen synthase kinase-3β inhibition in intracerebral hemorrhage rats." in: Journal of neurosurgery, pp. 1-9, 2016 (Sample species: Rat (Rattus)). Further details: Sample: brain

Zhang, Wu, Deng, Zheng, Zhang, Deng, Chen, Ma, Wang, Yu, Kang, Wang: "High altitude increases the expression of hypoxia-inducible factor 1α and inducible nitric oxide synthase with intest-inal mucosal barrier failure in rats." in: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 5189-95, 2016 Method employed by authors: Immunohistochemistry (IHC), Immunohistochemistry (IHC)

Guo, Deng, Cui, Peng, Fang, Zuo, Deng, Wang, Wu, Chen: "Nickel chloride (NiCl2)-caused inflammatory responses via activation of NF-κB pathway and reduction of anti-inflammatory mediator expression in the kidney." in: Oncotarget, Vol. 6, Issue 30, pp. 28607-20, 2016 Method employed by authors: Immunohistochemistry (IHC), Immunohistochemistry (IHC)

Li, Cheng, Zhao, Guo, Liu, Zhang, Wang, Zhang, Pan, Nie: "ADAMTS-7 exhibits elevated expression in cartilage of osteonecrosis of femoral head and has a positive correlation with TNF- α and NF- κ B P65." in: Mediators of inflammation, Vol. 2015, pp. 196702, 2016 Method employed by authors: Immunohistochemistry (IHC)

Qian, Cai, Zhang, Wang, Qian, Hasegawa: "Effect of Daisaikoto on Expressions of SIRT1 and NF-kappaB of Diabetic Fatty Liver Rats Induced by High-Fat Diet and Streptozotocin." in: Yonago acta medica, Vol. 59, Issue 2, pp. 149-58, 2016 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro))

Huang, Yang, Wang, Ma, Cui, Huang, Sheng, Weng, Xu: "Immunostimulatory activity of protein hydrolysate from oviductus ranae on macrophage in vitro." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 180234, 2015 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Luo, Yang, Tang, Yao, Kong, He, Zhou: "Kaempferol alleviates insulin resistance via hepatic IKK/NF-?B signal in type 2 diabetic rats." in: International immunopharmacology, Vol. 28, Issue 1, pp. 744-50, 2015 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)). Further details: Sample: Liver

Zhang, Huang, Guo, Gou, Hinsdale, Lloyd, Liu: "MicroRNA-26b Modulates the NF-κB Pathway in Alveolar Macrophages by Regulating PTEN." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, Issue 11, pp. 5404-14, 2015 Method employed by authors: Western Blotting (WB), Western Blotting (WB)

Images

Product Details anti-p65 Antibody Target Details p65 Application Details Handling References for anti-p65 antibody (ABIN668961) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit ...
Immunohistochemistry (IHC) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Formalin-fixed and paraffin-embedded rat aortic tissue labeled with Rabbit Anti-NFKB ...
Flow Cytometry (FACS) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Mouse splenocytes probed with Rabbit Anti-NFkB p65 Polyclonal Antibody, Unconjugated .
Western Blotting (WB) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) HCCLM3 lysates probed with Anti-NFKB p65 Polyclonal Antibody, Unconjugated at 1:5000...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti-NFKB p65 Pol...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Paraformaldehyde-fixed, paraffin embedded rat bladder, Antigen retrieval by boiling i...