anti-Human PRKACA antibody for Immunofluorescence (Cultured Cells)

Recommended PRKACA Antibody (supplied by: Log in to see )

Antigen
Protein Kinase A, alpha (PRKACA) Antibodies
  • PKCD
  • Pkaca
  • PKACA
  • PKA
  • Calpha
  • Cs-PKA
  • PKCA1
  • PRKAR1
  • protein kinase, cAMP dependent, catalytic, alpha
  • protein kinase, cAMP-dependent, catalytic, alpha
  • protein kinase, cAMP-dependent, regulatory, type I, beta
  • Prkaca
  • PRKACA
  • PRKAR1B
Epitope
AA 1-351
20
16
12
7
7
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
111
30
24
4
3
2
2
2
2
2
2
Host
Rabbit
62
43
6
1
Clonality
Polyclonal
Conjugate
This PRKACA antibody is un-conjugated
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF), Western Blotting (WB)
95
42
15
13
12
10
5
5
3
2
2
1
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Catalog No. ABIN2800195
$ 431.20
Plus shipping costs $45.00

General

Antigen Protein Kinase A, alpha (PRKACA) Antibodies
Epitope AA 1-351
(20), (16), (12), (7), (7), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(111), (30), (24), (4), (3), (2), (2), (2), (2), (2), (2)
Host Rabbit
(62), (43), (6), (1)
Clonality
Polyclonal
Conjugate This PRKACA antibody is un-conjugated
(4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF), Western Blotting (WB)
(95), (42), (15), (13), (12), (10), (5), (5), (3), (2), (2), (1)
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Product Details anti-PRKACA Antibody

Target Details PRKACA Application Details Handling Images
Immunogen Recombinant fragment corresponding to a region within amino acids 1-351 of PKA C alpha
Isotype IgG

Target Details PRKACA

Product Details anti-PRKACA Antibody Application Details Handling Images back to top
Antigen
Alternative Name PKA C alpha (PRKACA Antibody Abstract)
Background Synonyms: MGC102831 antibody, MGC48865 antibody, PKACA antibody, PRKACA antibody, protein kinase A catalytic subunit antibody, "cAMP-dependent protein kinase catalytic subunit alpha, isoform 1 antibody", cAMP-dependent protein kinase catalytic subunit alpha antibody, PKA C-alpha antibody, "protein kinase, cAMP-dependent, catalytic, alpha antibody"
Molecular Weight 41 kDa
UniProt P17612
Pathways NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals

Application Details

Product Details anti-PRKACA Antibody Target Details PRKACA Handling Images back to top
Application Notes Tested ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.Sample (30 μg of whole cell lysate) A: NIH-3T3 B: C2C12 10 % SDS PAGE 107-10691 diluted at 1:1000 Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PKA alpha(107-10691) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.Sample(30 μg of whole cell lysate)A:HeLa S312 % SDS PAGE107-10691 diluted at 1:500
Comment

PKA C alpha antibody, 1 mg/ml (100 μl)

Restrictions For Research Use only

Handling

Product Details anti-PRKACA Antibody Target Details PRKACA Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.