anti-Human PRKACA antibody for Western Blotting

Recommended PRKACA Antibody

Antigen
Protein Kinase A, alpha (PRKACA) Antibodies
  • PKACA
  • Calpha
  • Cs-PKA
  • PKCA1
  • PKA
  • PKCD
  • Pkaca
  • zgc:158799
  • protein kinase cAMP-activated catalytic subunit alpha
  • protein kinase, cAMP dependent, catalytic, alpha
  • protein kinase, cAMP-dependent, catalytic, alpha, genome duplicate a
  • PRKACA
  • Prkaca
  • prkacaa
Epitope
Center
12
11
8
7
6
5
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
101
34
28
4
3
2
2
2
2
2
2
Host
Rabbit
81
12
8
1
Clonality
Polyclonal
Conjugate
This PRKACA antibody is un-conjugated
4
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
73
32
19
13
10
7
6
3
3
2
2
1
1
Options

Available images

Catalog No. ABIN2855997
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
23.362497 ABIN2856513 ICC IF WB Rabbit IgG Center Polyclonal 0
23.362497 ABIN2683009 ELISA WB Mouse IgG1 AA 1-120 7H3A4 0
17.362497 ABIN633167 WB Rabbit N-Term Polyclonal 0
17.362497 ABIN929010 WB Rabbit N-Term Polyclonal 0
15.383289 ABIN545453 IHC (p) ELISA WB Rabbit Polyclonal 3
13.479209 ABIN5539751 ELISA WB Goat Internal Region Polyclonal 0
13.479209 ABIN5539752 ELISA WB Goat Internal Region Polyclonal 0
13.479209 ABIN6136200 WB Rabbit pSer339 Polyclonal 0
13.479209 ABIN6136202 WB Rabbit pThr197 Polyclonal 0
13.479209 ABIN2149655 ELISA WB Rabbit IgG Polyclonal 0
13.479209 ABIN6134415 IHC WB Rabbit Polyclonal 0
10.883289 ABIN5586147 ELISA IF IHC (p) WB Rabbit Polyclonal 0
10.883289 ABIN5586148 IF IHC (p) WB Rabbit pThr197 Polyclonal 0
10.883289 ABIN3002818 FACS IHC WB Rabbit IgG Polyclonal 0
10 ABIN5534621 FACS IHC (p) WB Rabbit Ig Fraction AA 67-94, N-Term Polyclonal 0
10 ABIN519175 PLA ELISA WB Mouse IgG1 kappa AA 1-120 1D7 0
8.5 ABIN562366 ELISA WB Mouse AA 1-120 Polyclonal 1
8.5 ABIN535235 DB WB Mouse IgG 6D2-1 3
7.8832893 ABIN2787415 IHC WB Rabbit N-Term Polyclonal 0
7.8832893 ABIN2787416 IHC WB Rabbit N-Term Polyclonal 0

General

Antigen Protein Kinase A, alpha (PRKACA) Antibodies
Epitope Center
(12), (11), (8), (7), (6), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(101), (34), (28), (4), (3), (2), (2), (2), (2), (2), (2)
Host Rabbit
(81), (12), (8), (1)
Clonality
Polyclonal
Conjugate This PRKACA antibody is un-conjugated
(4), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(73), (32), (19), (13), (10), (7), (6), (3), (3), (2), (2), (1), (1)
Supplier

Product Details anti-PRKACA Antibody

Target Details PRKACA Application Details Handling Images
Cross-Reactivity Rat (Rattus), Dog (Canine), Sheep (Ovine), Cow (Bovine)
Cross-Reactivity (Details) Rat (100 %), Dog (100 %), Sheep (100 %), Bovine (100 %)
Characteristics Rabbit Polyclonal antibody to PKA C alpha (protein kinase, cAMP-dependent, catalytic, alpha)
PKA C alpha antibody [N2C3]
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human PKA C alpha. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details PRKACA

Product Details anti-PRKACA Antibody Application Details Handling Images back to top
Antigen
Alternative Name PKA C alpha (PRKACA Antibody Abstract)
Background CAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Cellular Localization: Cytoplasm (By similarity) , Nucleus (By similarity)
Molecular Weight 41 kDa
Gene ID 5566
Pathways NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, Lipid Metabolism

Application Details

Product Details anti-PRKACA Antibody Target Details PRKACA Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: HeLa , Jurkat , NT2D1 , mouse brain

Restrictions For Research Use only

Handling

Product Details anti-PRKACA Antibody Target Details PRKACA Application Details Images back to top
Format Liquid
Concentration 0.38 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-PRKACA Antibody Target Details PRKACA Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Protein Kinase A, alpha (PRKACA) (Center) antibody (ABIN2855997) WB Image Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE antibody diluted at...
Western Blotting (WB) image for anti-Protein Kinase A, alpha (PRKACA) (Center) antibody (ABIN2855997) WB Image Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE antibody dil...
Immunohistochemistry (IHC) image for anti-Protein Kinase A, alpha (PRKACA) (Center) antibody (ABIN2855997) IHC-P Image Immunohistochemical analysis of paraffin-embedded human colon, using PKA ...
Immunofluorescence (IF) image for anti-Protein Kinase A, alpha (PRKACA) (Center) antibody (ABIN2855997) ICC/IF Image Immunofluorescence analysis of methanol-fixed HeLa, using PKA alpha, ant...