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|Antigen||RANK (CD265) Antibodies|
|Conjugate||This RANK antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
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Product Details anti-RANK AntibodyTarget Details RANK Application Details Handling Images
|Specificity||Human sRANK Receptor|
E.coli derived recombinant Human sRANK Receptor.
Type of Immunogen: Cell extract
Target Details RANKProduct Details anti-RANK Antibody Application Details Handling Images back to top
|Alternative Name||TNFRSF11A / RANK (CD265 Antibody Abstract)|
Name/Gene ID: TNFRSF11A
Family: TNF Receptor
Synonyms: TNFRSF11A, CD265, CD265 antigen, ODFR, LOH18CR1, OPTB7, OSTS, PDB2, RANK, Receptor activator of NF-KB, TRANCER, FEO, OFE
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Approved: ELISA, IHC-P (1 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 1 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hsRANK Receptor by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sRANK Receptor (LS-C104414) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsRANK Receptor. Western Blot: To detect hsRANK Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANK Receptor is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
|Restrictions||For Research Use only|
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|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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