anti-Cow (Bovine) TGFA antibody for Immunofluorescence

Recommended TGFA Antibody (supplied by: Log in to see )

Antigen
Transforming Growth Factor, alpha (TGFA) Antibodies
  • TFGA
  • LOC100009482
  • TGF alpha
  • TGFA
  • wa-1
  • wa1
  • RATTGFAA
  • TGFAA
  • NV15606
  • si:ch211-10e8.7
  • transforming growth factor alpha
  • transforming growth factor alpha L homeolog
  • transforming growth factor-alpha
  • transforming growth factor, alpha
  • TGFA
  • TGF-A
  • Tgfa
  • tgfa.L
  • LOC100121300
  • LOC100009150
  • tgfa
Epitope
C-Term
188
93
16
8
7
5
5
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
484
204
200
120
101
57
9
3
3
2
1
Host
Mouse
337
157
16
4
2
Clonality
Monoclonal
Conjugate
This TGFA antibody is conjugated to CF®555
33
19
15
15
10
8
8
8
7
7
7
7
7
7
7
7
7
7
7
7
6
6
6
6
6
6
6
6
6
6
6
6
5
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
311
282
215
176
172
168
110
110
52
20
18
13
13
8
7
4
4
2
2
1
1
Supplier
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Catalog No. ABIN6171382
$ 209.23
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6171380 FACS IF IHC (f) CF®647 Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171386 FACS IF IHC (f) CF®568 Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171390 FACS IF IHC (f) CF®680 Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171392 FACS IF IHC (f) CF®680R Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171376 FACS IF IHC (f) CF®640R Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171378 FACS IF IHC (f) CF®543 Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171384 FACS IF IHC (f) CF®660R Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171388 FACS IF IHC (f) CF®770 Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171396 FACS IF IHC (f) CF®594 Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171372 FACS IF IHC (f) CF®405S Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171374 FACS IF IHC (f) CF405M Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171394 FACS IF IHC (f) CF®488A Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171407 FACS IF IHC (f) Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171399 FACS IF IHC (f) Alkaline Phosphatase (AP) Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171405 FACS IF IHC (f) PerCP Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171398 FACS IF IHC (f) APC Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171401 FACS IF IHC (f) Biotin Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171406 FACS IF IHC (f) PE Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171409 FACS IF IHC (f) Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171403 FACS IF IHC (f) HRP Mouse IgG1, kappa C-Term Log in to see MF9 0

General

Antigen Transforming Growth Factor, alpha (TGFA) Antibodies
Epitope C-Term
(188), (93), (16), (8), (7), (5), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(484), (204), (200), (120), (101), (57), (9), (3), (3), (2), (1)
Host Mouse
(337), (157), (16), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TGFA antibody is conjugated to CF®555
(33), (19), (15), (15), (10), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
(311), (282), (215), (176), (172), (168), (110), (110), (52), (20), (18), (13), (13), (8), (7), (4), (4), (2), (2), (1), (1)
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Product Details anti-TGFA Antibody

Target Details TGFA Application Details Handling Images
Purpose Mouse Monoclonal anti-TGF alpha (MF9), CF555 Conjugate
Characteristics This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF? ? (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF?.
Clone MF9
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details TGFA

Product Details anti-TGFA Antibody Application Details Handling Images back to top
Antigen
Alternative Name TGF alpha (TGFA Antibody Abstract)
Background EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1
Molecular Weight 6 kDa
Gene ID 7039, 170009
UniProt P01135
Pathways NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-TGFA Antibody Target Details TGFA Handling Images back to top
Application Notes Immunohistology formalin-fixed 2-4 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Immunofluorescence 1-2 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

Jurkat or Ramos cells. Testicular Carcinoma

Restrictions For Research Use only

Handling

Product Details anti-TGFA Antibody Target Details TGFA Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light