anti-Zebrafish (Danio rerio) TGFA antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended TGFA Antibody (supplied by: Log in to see )

Antigen
Transforming Growth Factor, alpha (TGFA) Antibodies
  • TFGA
  • LOC100009482
  • TGF alpha
  • TGFA
  • wa-1
  • wa1
  • RATTGFAA
  • TGFAA
  • NV15606
  • si:ch211-10e8.7
  • transforming growth factor alpha
  • transforming growth factor alpha L homeolog
  • transforming growth factor-alpha
  • transforming growth factor, alpha
  • TGFA
  • TGF-A
  • Tgfa
  • tgfa.L
  • LOC100121300
  • LOC100009150
  • tgfa
Epitope
AA 34-43
176
93
16
8
7
5
5
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Rabbit, Zebrafish (Danio rerio)
467
191
187
117
101
56
9
3
3
2
1
Host
Mouse
321
157
12
4
2
Clonality
Monoclonal
Conjugate
This TGFA antibody is un-conjugated
31
18
15
14
10
8
8
7
7
7
7
7
7
7
7
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
299
271
216
170
159
150
99
96
45
21
17
13
13
5
4
4
2
2
1
1
Supplier
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Catalog No. ABIN3026446
$ 471.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
4 ABIN3026163 FACS IF IHC (p) Mouse IgM, kappa AA 34-43 Log in to see 1E8-G6 0
4 ABIN3026450 FACS IF IHC (p) Mouse IgM AA 34-43 Log in to see TG86-P-T1 0
4 ABIN3026448 FACS IF IHC (p) Mouse IgG1, kappa AA 34-43 Log in to see P-T1 0
4 ABIN3026452 FACS IF IHC (p) Mouse IgG1, kappa AA 34-43 Log in to see TG86 0
1 ABIN2744784 ELISA FACS IF IHC IHC (p) Mouse kappa, IgM AA 34-43 Log in to see 1E8-G6 0
1 ABIN6252279 FACS IF IHC (p) Mouse IgG1, kappa AA 34-43 Log in to see TG86 0
1 ABIN3026164 FACS IF IHC (p) Mouse IgM, kappa AA 34-43 Log in to see 1E8-G6 0
1 ABIN3026449 FACS IF IHC (p) Mouse IgG1, kappa AA 34-43 Log in to see P-T1 0
1 ABIN3026447 FACS IF IHC (p) Mouse IgM, kappa AA 34-43 Log in to see SPM542 0
1 ABIN3026451 FACS IF IHC (p) Mouse IgM AA 34-43 Log in to see TG86-P-T1 0
1 ABIN3026453 FACS IF IHC (p) Mouse IgG1, kappa AA 34-43 Log in to see TG86 0
-9.842285 ABIN4358991 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgM Log in to see TG86-P-T1 0
-12.842285 ABIN4358885 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa AA 34-43 Log in to see TG86 0
-12.842285 ABIN4358888 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa AA 34-43 Log in to see P-T1 0
-12.842285 ABIN4358894 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa AA 34-43 Log in to see SPM542 0
-12.842285 ABIN4358882 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa AA 34-43 Log in to see 1E8-G6 0
-12.842285 ABIN4358910 ELISA FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 700 Mouse IgM kappa AA 34-43 Log in to see SPM542 0
-12.842285 ABIN4358915 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 488 Mouse IgM kappa AA 34-43 Log in to see SPM542 0
-12.842285 ABIN4358912 ELISA ICC IF IHC (fro) IHC (p) Biotin Mouse IgM kappa AA 34-43 Log in to see SPM542 0
-12.842285 ABIN4358918 FACS ICC IF IHC (fro) IHC (p) IP PerCP Mouse IgM kappa AA 34-43 Log in to see SPM542 0

General

Antigen Transforming Growth Factor, alpha (TGFA) Antibodies
Epitope AA 34-43
(176), (93), (16), (8), (7), (5), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Rabbit, Zebrafish (Danio rerio)
(467), (191), (187), (117), (101), (56), (9), (3), (3), (2), (1)
Host Mouse
(321), (157), (12), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TGFA antibody is un-conjugated
(31), (18), (15), (14), (10), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(299), (271), (216), (170), (159), (150), (99), (96), (45), (21), (17), (13), (13), (5), (4), (4), (2), (2), (1), (1)
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Product Details anti-TGFA Antibody

Target Details TGFA Application Details Handling Images
Characteristics This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. TGFa is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Purification Protein G affinity chromatography
Immunogen Amino acids 34-43 (PPVAAAVVSH) from the human protein were used as the immunogen for this anti-TGF alpha antibody.
Clone SPM542
Isotype IgM, kappa
Plasmids, Primers & others

Target Details TGFA

Product Details anti-TGFA Antibody Application Details Handling Images back to top
Antigen
Alternative Name TGF-alpha (TGFA Antibody Abstract)
Background This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. TGFa is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Pathways NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-TGFA Antibody Target Details TGFA Handling Images back to top
Application Notes The optimal dilution of the anti-TGF-alpha antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1)

Restrictions For Research Use only

Handling

Product Details anti-TGFA Antibody Target Details TGFA Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the anti-TGF-alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-TGFA Antibody Target Details TGFA Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Transforming Growth Factor, alpha (TGFA) (AA 34-43) antibody (ABIN3026446) Formalin-fixed, paraffin-embedded human testicular carcinoma stained with anti-TGF al...