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|Antigen||Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) Antibodies|
|Conjugate||This TNFRSF1A antibody is un-conjugated Alternatives|
Agonist (Agon), Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-TNFRSF1A AntibodyTarget Details TNFRSF1A Application Details Handling References for anti-TNFRSF1A antibody (ABIN5013005) Images
|Specificity||Detects human sTNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 10 % cross-reactivity with recombinant mouse TNF sRI is observed. Additionally, in direct ELISAs, this antibody does not cross-react with recombinant human (rh) sTNF RII, recombinant mouse (rm) sTNF RII, rhTNF-alpha , rmTNF-alpha , rhTNF-beta . This anti-human TNF RI antibody can interact with the cell surface 55 kDa human TNF RI and exhibits TNF agonist activities on the A549 human lung carcinoma cell line. This antibody also exhibits TNF agonist activities on the L-929 mouse fibroblast cell line, suggesting that it has cross-species activity with mouse cell surface TNF RI.|
|Purification||Immunogen affinity purified|
|Endotoxin Level||<0.10 EU per 1 μg of the antibody by the LAL method.|
|Immunogen||E. coli-derived recombinant human sTNF RI/TNFRSF1A, Accession # NP_001056|
Target Details TNFRSF1AProduct Details anti-TNFRSF1A Antibody Application Details Handling References for anti-TNFRSF1A antibody (ABIN5013005) Images back to top
|Alternative Name||TNF RI/TNFRSF1A (TNFRSF1A Antibody Abstract)|
|Background||Alternative Names: CD120a, CD120a antigen, FPF, p55, p55-R, p60, TNFARMGC19588, TNF-R, TNF-R1, TNFR1TBP1, TNFR55, TNF-R55, TNFR60, TNFRI, TNF-RI, TNF-R-I, TNFR-I, TNFRSF1A, tumor necrosis factor binding protein 1, Tumor necrosis factor receptor 1, tumor necrosis factor receptor 1A isoform beta, tumor necrosis factor receptor superfamily member 1A, tumor necrosis factor receptor superfamily, member 1A, tumor necrosis factor receptor type 1, Tumor necrosis factor receptor type I, tumor necrosis factor-alpha receptor|
|Pathways||NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C|
Application DetailsProduct Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Handling References for anti-TNFRSF1A antibody (ABIN5013005) Images back to top
Western Blot 0.1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Agonist Activity 1-6 μg/mL
Immunocytochemistry 5-15 μg/mL
|Restrictions||For Research Use only|
HandlingProduct Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Application Details References for anti-TNFRSF1A antibody (ABIN5013005) Images back to top
|Reconstitution||Reconstitute at 0.2 mg/mL in sterile PBS.|
|Buffer||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Storage||4 °C,-20 °C,-80 °C|
|Storage Comment||Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.|
|Expiry Date||12 months|
References for anti-TNFRSF1A antibody (ABIN5013005)Product Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Application Details Handling Images back to top
|Product cited in:||
Wang, Tan, Mu, Chang, Wu, Tu, Zhang, Guo, Qin, Li, Li, Li, Zhang, Li: "Ubiquitin-associated domain-containing ubiquitin regulatory X (UBX) protein UBXN1 is a negative regulator of nuclear factor κB (NF-κB) signaling." in: The Journal of biological chemistry, Vol. 290, Issue 16, pp. 10395-405, 2015 Method employed by authors: Immunoprecipitation (IP) (Sample species: Human).
Luci, Bekri, Bihl, Pini, Bourdely, Nouhen, Malgogne, Walzer, Braud, Anjuère: "NKp46+ Innate Lymphoid Cells Dampen Vaginal CD8 T Cell Responses following Local Immunization with a Cholera Toxin-Based Vaccine." in: PLoS ONE, Vol. 10, Issue 12, pp. e0143224, 2015 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).
Zhao, Qureshi, Eastman, Manning, Alexander, Robinson, Hesterberg: "Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis." in: Journal of immunological methods, Vol. 378, Issue 1-2, pp. 72-80, 2012 Method employed by authors: ELISA (ELISA) (Sample species: Human).
Hadjadj, Cloutier, Geoffroy, Tanguay: "Stent implantation in coronary porcine arteries is associated with early activation of TNFalpha and TNFalpha receptor II expression." in: Atherosclerosis, Vol. 192, Issue 1, pp. 25-32, 2007 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Pig (Porcine)).
van Grevenstein, Hofland, van Rossen, van Koetsveld, Jeekel, van Eijck: "Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers." in: Digestive diseases and sciences, Vol. 52, Issue 10, pp. 2775-83, 2007 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Human).
ImagesProduct Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Application Details Handling References for anti-TNFRSF1A antibody (ABIN5013005) back to top