anti-Mouse (Murine) TNFRSF1A antibody for Functional Studies

Recommended TNFRSF1A Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) Antibodies
  • CD120a
  • FPF
  • MS5
  • TBP1
  • TNF-R
  • TNF-R-I
  • TNF-R55
  • TNFAR
  • TNFR1
  • TNFR1-d2
  • TNFR55
  • TNFR60
  • p55
  • p55-R
  • p60
  • TNF-R1
  • TNF-alphaR1
  • TNFRI
  • TNFRp55
  • TNFalpha-R1
  • Tnfr-2
  • Tnfr1
  • fpf
  • tbp1
  • p55-r
  • tnf-r
  • tnfar
  • tnfr1
  • cd120a
  • tnfr55
  • tnfr60
  • xtnfr1
  • wu:fj65b10
  • zgc:85914
  • TNFR I
  • TNFRSF1A
  • TNFA
  • cTNF
  • TNF receptor superfamily member 1A
  • tumor necrosis factor receptor superfamily, member 1a
  • TNF receptor superfamily member 1A S homeolog
  • tumor necrosis factor
  • TNFRSF1A
  • Tnfrsf1a
  • tnfrsf1a.S
  • tnfrsf1a
  • TNF
Reactivity
Mouse (Murine)
351
184
130
75
70
67
62
13
10
6
6
5
5
3
2
1
1
1
Host
Rabbit
275
97
17
15
15
2
1
Clonality
Polyclonal
Conjugate
This TNFRSF1A antibody is un-conjugated
33
27
26
15
10
10
6
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunoassay (IA), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
282
167
138
123
117
60
42
32
29
27
23
18
13
10
6
5
4
3
3
2
2
1
1
1
1
Options
Supplier
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Supplier Product No.
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Catalog No. ABIN2192161
$ 715.97
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.280954 ABIN2191848 Func IA FACS IP FITC Armenian Hamster Log in to see 55R-170 2
13.280954 ABIN2192210 Func IA FACS IP Armenian Hamster Log in to see 55R-170 2
1 ABIN1830498 Func FACS IP ELISA FITC Hamster IgG1 Extracellular Domain Log in to see 13A86 0
1 ABIN1105788 Func EIA IP Hamster IgG Extracellular Domain Log in to see 55R-170 4
1 ABIN1105796 Func EIA IP FITC Hamster IgG Extracellular Domain Log in to see 55R-170 4
1 ABIN1105789 Func EIA IP WB Rabbit IgG Log in to see Polyclonal 3

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) Antibodies
Reactivity Mouse (Murine)
(351), (184), (130), (75), (70), (67), (62), (13), (10), (6), (6), (5), (5), (3), (2), (1), (1), (1)
Host Rabbit
(275), (97), (17), (15), (15), (2), (1)
Clonality
Polyclonal
Conjugate This TNFRSF1A antibody is un-conjugated
(33), (27), (26), (15), (10), (10), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunoassay (IA), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(282), (167), (138), (123), (117), (60), (42), (32), (29), (27), (23), (18), (13), (10), (6), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-TNFRSF1A Antibody

Target Details TNFRSF1A Application Details Handling References for anti-TNFRSF1A antibody (ABIN2192161) Images
Cross-Reactivity (Details) Cross reactivity: Mouse TNF-RII : Yes, Mouse TNF-alpha Minimal crossreactivity problematic in case of TNF-RI knockout studies and when used in high concentrations in functional studies
Sterility 0.2 μm filtered
Isotype IgG
Plasmids, Primers & others

Target Details TNFRSF1A

Product Details anti-TNFRSF1A Antibody Application Details Handling References for anti-TNFRSF1A antibody (ABIN2192161) Images back to top
Antigen
Alternative Name Cd120a, Tnf-Ri (TNFRSF1A Antibody Abstract)
Background The polyclonal antibody recognizes the extracellular part of the mouse Tumor Necrosis Factor Receptor type 1 (TNF-RI) of the membrane-bound as well as the soluble receptor. TNF-RI (~55-60 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-RI and TNF-RII, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is efficiently activated only by the membrane bound form of TNF-alpha. TNF-RI signaling is initiated when trimeric TNF-alpha binds TNF-RI receptors. Subsequent TNF-RI trimerization promotes the recruitment of a proximal signaling complex composed of TNF Receptor Associated protein with a Death Domain (TRADD), Receptor Interacting Protein (RIP), cellular Inhibitor of Apoptosis Protein 1 (cIAP1), TNF Receptor Associated Factor 2 (TRAF2), and likely TRAF5. Studies with TNF-RI-deficient mice indicate that TNF-RI mediates most of the proliferation, pro-inflammatory, and apoptosis-activating pathways. Aliases Tumor necrosis factor receptor superfamily member 1A, TNF-RI, p55/p60
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C

Application Details

Product Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Handling References for anti-TNFRSF1A antibody (ABIN2192161) Images back to top
Application Notes For flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Antibody reactivity with cell bound or soluble TNF-RI is not inhibited by high concentrations of mouse TNFα.
Restrictions For Research Use only

Handling

Product Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Application Details References for anti-TNFRSF1A antibody (ABIN2192161) Images back to top
Buffer PBS, containing 0.1 % bovine serum albumin
Storage 4 °C
Storage Comment Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Expiry Date 12 months

References for anti-TNFRSF1A antibody (ABIN2192161)

Product Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Application Details Handling Images back to top
Product cited in:

Gerspach, Götz, Zimmermann, Kolle, Böttinger, Grell: "Detection of membrane-bound tumor necrosis factor (TNF): an analysis of TNF-specific reagents." in: Microscopy research and technique, Vol. 50, Issue 3, pp. 243-50, 2000

Lucas, Garcia, Donati, Hribar, Mandriota, Giroud, Buurman, Fransen, Suter, Nunez, Pepper, Grau: "Both TNF receptors are required for direct TNF-mediated cytotoxicity in microvascular endothelial cells." in: European journal of immunology, Vol. 28, Issue 11, pp. 3577-86, 1998

Bemelmans, Gouma, Buurman: "LPS-induced sTNF-receptor release in vivo in a murine model. Investigation of the role of tumor necrosis factor, IL-1, leukemia inhibiting factor, and IFN-gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 10, pp. 5554-62, 1993