anti-Mouse (Murine) TNFRSF1B antibody for Flow Cytometry

Recommended TNFRSF1B Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) Antibodies
  • CD120b
  • TBPII
  • TNF-R-II
  • TNF-R75
  • TNFBR
  • TNFR1B
  • TNFR2
  • TNFR80
  • p75
  • p75TNFR
  • TNF-RII/TNFRSF1B
  • TNFR-II
  • TNFRSF1B
  • TNF-R2
  • TNF-alphaR2
  • TNFRII
  • TNFalpha-R2
  • Tnfr-1
  • Tnfr2
  • rank
  • zgc:163064
  • TNF receptor superfamily member 1B
  • tumor necrosis factor receptor superfamily, member 1b
  • tumor necrosis factor receptor superfamily, member 1B
  • TNFRSF1B
  • Tnfrsf1b
  • tnfrsf1b
Reactivity
Mouse (Murine)
176
134
61
6
3
2
2
2
2
2
2
Host
Rat
136
70
21
17
16
11
1
Clonality
Monoclonal
Conjugate
This TNFRSF1B antibody is conjugated to FITC
29
12
9
7
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunoassay (IA), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
175
101
99
63
55
27
25
25
21
19
14
13
9
8
5
3
3
2
1
1
1
1
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Catalog No. ABIN2191751
$ 864.51
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
25.436094 ABIN2191752 Func IA FACS IP WB Biotin Rat Log in to see HM102 2
25.436094 ABIN2191750 Func IA FACS IP WB Rat Log in to see HM102 2
21.36386 ABIN4899742 CyTOF FACS ICC WB Goat IgG AA 23-258 Log in to see Polyclonal 7
17.072235 ABIN2192162 Func IA FACS IP WB Rabbit IgG Log in to see Polyclonal 3
13.863859 ABIN4900547 CyTOF ELISA (Capture) FACS WB Hamster IgG Extracellular Domain Log in to see TR7554 4
13.863859 ABIN4897939 FACS APC Hamster IgG Extracellular Domain Log in to see TR7554 1
9.363859 ABIN2660924 FACS IHC ELISA Biotin Hamster IgG Log in to see TR75-89 7
9.363859 ABIN2665529 FACS ICFC Hamster IgG Log in to see TR75-32-4 6
9.363859 ABIN2693139 FACS APC Rabbit IgG AA 1-258 Log in to see 112 0
9.363859 ABIN2693141 FACS PE Rabbit IgG AA 1-258 Log in to see 112 0
9.363859 ABIN2665530 FACS Hamster IgG Log in to see TR75-54-7 5
9.363859 ABIN2660925 Func FACS IHC ELISA Biotin Hamster IgG Log in to see TR75-32-4 5
9.363859 ABIN5508643 FACS Rabbit IgG AA 1-258 Log in to see 112 0
5.5 ABIN2688928 FACS Biotin Armenian Hamster IgG1, lambda Log in to see TR75 1
1 ABIN602493 ELISA FACS IHC IHC (fro) FITC Rat IgG2a Log in to see 0
1 ABIN1105801 EIA IHC (fro) FACS IP Rat IgG2a Log in to see HM102 2
1 ABIN215526 FACS IHC IHC (fro) IP Biotin Rat IgG2a Log in to see 0
1 ABIN602497 ELISA FACS IHC IHC (fro) IP Rat IgG2a Log in to see HM102 0
1 ABIN1889572 FACS WB Hamster IgG AA 23-258 Log in to see RM0012-6F13 0
1 ABIN5500423 FACS Alexa Fluor 594 Hamster IgG Log in to see TR7554 0

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) Antibodies
Reactivity Mouse (Murine)
(176), (134), (61), (6), (3), (2), (2), (2), (2), (2), (2)
Host Rat
(136), (70), (21), (17), (16), (11), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TNFRSF1B antibody is conjugated to FITC
(29), (12), (9), (7), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunoassay (IA), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(175), (101), (99), (63), (55), (27), (25), (25), (21), (19), (14), (13), (9), (8), (5), (3), (3), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-TNFRSF1B Antibody

Target Details TNFRSF1B Application Details Handling References for anti-TNFRSF1B antibody (ABIN2191751) Images
Sterility 0.2 μm filtered
Clone HM102
Plasmids, Primers & others

Target Details TNFRSF1B

Product Details anti-TNFRSF1B Antibody Application Details Handling References for anti-TNFRSF1B antibody (ABIN2191751) Images back to top
Antigen
Alternative Name Tnf-Rii (TNFRSF1B Antibody Abstract)
Background The monoclonal antibody HM102 recognizes the extracellular part of membrane-bound TNF-RII as well as the soluble form of TNF-RII which is generated by proteolytic cleavage of the extracellular domain. The soluble form can still bind TNF-alpha with high affinity and functions as a TNF-alpha antagonist. TNF-alpha is an important signalling protein in the immune system which can activate inflammatory responses, induce apoptosis, regulate cellular proliferation, and may even promote cancer progression. TNF-alpha can bind to two structurally distinct membrane receptors, TNF-RI and TNFRII, which have both distinct and overlapping downstream signaling cascades. TNFRI is believed to be expressed on nearly all cell types, whereas TNFRII exhibits more restricted expression, being found on certain subpopulations of immune cells and several other cell types. A dominant role of TNFRII has been shown in thymocyte activation by TNF-alpha, whereas induction of cytotoxicity and other functions are mediated largely by TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is activated only by the membrane bound form of TNF-alpha. The antibody is an agonistic receptor modulating antibody. It enhances in vitro TNF alpha responses by increasing the affinity of the soluble form of TNF-alpha for TNF-RII. Aliases Tumor necrosis factor receptor superfamily member 1B, CD120b, TNF-R2, p75, p80 TNF-alpha receptor
Pathways NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C

Application Details

Product Details anti-TNFRSF1B Antibody Target Details TNFRSF1B Handling References for anti-TNFRSF1B antibody (ABIN2191751) Images back to top
Application Notes For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive RAW264.7 cells control
Restrictions For Research Use only

Handling

Product Details anti-TNFRSF1B Antibody Target Details TNFRSF1B Application Details References for anti-TNFRSF1B antibody (ABIN2191751) Images back to top
Buffer PBS, containing 1 % bovine serum albumin.
Storage 4 °C
Storage Comment Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-TNFRSF1B antibody (ABIN2191751)

Product Details anti-TNFRSF1B Antibody Target Details TNFRSF1B Application Details Handling Images back to top
Product cited in:

Brekke, Sagen, Bjerve: "Specificity of endogenous fatty acid release during tumor necrosis factor-induced apoptosis in WEHI 164 fibrosarcoma cells." in: Journal of lipid research, Vol. 40, Issue 12, pp. 2223-33, 2000

Tacchini-Cottier, Vesin, Redard, Buurman, Piguet: "Role of TNFR1 and TNFR2 in TNF-induced platelet consumption in mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 12, pp. 6182-6, 1998

Images

Product Details anti-TNFRSF1B Antibody Target Details TNFRSF1B Application Details Handling References for anti-TNFRSF1B antibody (ABIN2191751) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) antibody (FITC) (ABIN2191751) Flow cytometric detection of mouse TNF-RII (5µg/ml) on 500,000 BV2 microglial cells (...