Chicken TNFSF13B ELISA Kit

Recommended TNFSF13B Product (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) ELISA Kits
  • RGD1561519
  • TNFSF13B
  • BAFF
  • BLYS
  • CD257
  • DTL
  • TALL-1
  • TALL1
  • THANK
  • TNFSF20
  • ZTNF4
  • BLyS
  • D8Ertd387e
  • zTNF4
  • tumor necrosis factor (ligand) superfamily, member 13b
  • Tnfsf13b
  • TNFSF13B
Reactivity
Chicken
Alternatives
Kits with alternative reactivity to:
27
22
8
2
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
78-5.000 pg/mL
Minimum Detection Limit
78 pg/mL
Application
ELISA
Options
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Catalog No. ABIN5564666
$ 608.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN1559535 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see

General

Antigen Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) ELISA Kits
Reactivity Chicken
Kits with alternative reactivity to:
(27), (22), (8), (2), (1), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 78-5.000 pg/mL
Minimum Detection Limit 78 pg/mL
Application ELISA
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Product Details TNFSF13B ELISA Kit

Target details Application Details Handling Images
Sample Type Biological Fluids, Cell Lysate, Plasma, Serum, Tissue Homogenate
Detection Method Colorimetric
Analytical Method Quantitative
Specificity

This assay has high sensitivity and excellent specificity for detection of B-Cell Activating Factor (BAFF).
No significant cross-reactivity or interference between B-Cell Activating Factor (BAFF) and analogues was observed.

Sensitivity 27 pg/mL
Material not included
  • Microplate reader with 450 nm filter.
  • Precision single or multi-channel pipettes and disposable tips.
  • Eppendorf Tubes for diluting samples.
  • Deionized or distilled water.
  • Absorbent paper for blotting the microtiter plate.
  • Container for Wash Solution

Target details

Product Details TNFSF13B ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name B-Cell Activating Factor (BAFF) (TNFSF13B ELISA Kit Abstract)
Pathways NF-kappaB Signaling, Production of Molecular Mediator of Immune Response

Application Details

Product Details TNFSF13B ELISA Kit Target details Handling Images back to top
Assay Time 3 h
Plate Pre-coated
Protocol The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell Activating Factor (BAFF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell Activating Factor (BAFF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell Activating Factor (BAFF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell Activating Factor (BAFF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Procedure
  1. Prepare all reagents, samples and standards,
  2. Add 100μL standard or sample to each well. Incubate 2 hours at 37 °C,
  3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
  4. Aspirate and wash 3 times,
  5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
  6. Aspirate and wash 5 times,
  7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
  8. Add 50μL Stop Solution. Read at 450nm immediately.
Assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Cell Activating Factor (BAFF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Cell Activating Factor (BAFF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions For Research Use only

Handling

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Handling Advice

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Storage 4 °C

Images

Product Details TNFSF13B ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) ELISA Kit (ABIN5564666) Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) ELISA Kit