anti-Cat (Feline) Tumor Necrosis Factor antibody for Immunoprecipitation

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LOC103694380
  • tnfa
Reactivity
Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
1361
521
453
220
216
196
195
59
56
44
32
18
15
9
7
6
6
5
4
4
4
4
3
2
2
1
1
1
1
Host
Mouse
1047
544
138
52
11
11
8
7
5
2
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to Alexa Fluor 488
182
94
69
54
49
23
22
22
20
20
18
18
18
18
18
18
18
17
11
11
11
11
11
11
11
11
11
11
11
11
11
10
10
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
810
808
747
465
397
260
196
162
131
113
98
67
48
42
27
26
25
17
13
9
9
9
8
7
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN4360976
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
-11.561594 ABIN4360978 ELISA FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 700 Mouse IgG1 kappa Log in to see SPM543 0
-11.561594 ABIN4360981 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 650 Mouse IgG1 kappa Log in to see SPM543 0
-11.561594 ABIN4360989 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 350 Mouse IgG1 kappa Log in to see SPM543 0
-11.561594 ABIN4360977 ELISA FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 647 Mouse IgG1 kappa Log in to see SPM543 0
-11.561594 ABIN4360983 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 488 Mouse IgG1 kappa Log in to see SPM543 0
-11.561594 ABIN4360985 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 755 Mouse IgG1 kappa Log in to see SPM543 0
-11.561594 ABIN4360986 FACS ICC IF IHC (fro) IHC (p) IP PerCP Mouse IgG1 kappa Log in to see SPM543 0
-11.561594 ABIN4360988 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 550 Mouse IgG1 kappa Log in to see SPM543 0
-11.561594 ABIN4360990 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 405 Mouse IgG1 kappa Log in to see SPM543 0
-11.561594 ABIN4360975 ELISA FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 405 Mouse IgG1 kappa Log in to see SPM543 0
-11.561594 ABIN4360982 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 680 Mouse IgG1 kappa Log in to see SPM543 0

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(1361), (521), (453), (220), (216), (196), (195), (59), (56), (44), (32), (18), (15), (9), (7), (6), (6), (5), (4), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(1047), (544), (138), (52), (11), (11), (8), (7), (5), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to Alexa Fluor 488
(182), (94), (69), (54), (49), (23), (22), (22), (20), (20), (18), (18), (18), (18), (18), (18), (18), (17), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (10), (10), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(810), (808), (747), (465), (397), (260), (196), (162), (131), (113), (98), (67), (48), (42), (27), (26), (25), (17), (13), (9), (9), (9), (8), (7), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Specificity Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Purification Protein A purified
Immunogen A hexadecapeptide corresponding to aa115-130 of human TNF-alpha, conjugated to thyroglobulin
Clone SPM543
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Gene Symbol: TNF
Molecular Weight Theoretical MW: 17 kDa
Gene ID 7124
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-FrozenImmunohistochemistry (Staining of paraffin tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Buffer 50 mM Sodium Borate
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C in the dark.

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor (TNF) antibody (Alexa Fluor 488) (ABIN4360976) Flow Cytometry: TNF-alpha Antibody (SPM543) - An intracellular stain was performed ...