anti-Ferret Tumor Necrosis Factor antibody for ELISA (Detection)

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Epitope
AA 77-233
90
68
52
48
25
25
25
19
17
15
10
10
8
7
7
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Ferret
1503
553
484
225
217
198
197
62
55
42
31
18
13
13
9
7
5
4
4
4
3
3
2
1
1
1
Host
Mouse
1150
573
178
48
11
10
8
7
5
2
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
192
103
83
63
61
39
33
31
28
26
26
26
26
25
25
25
24
20
13
11
11
11
11
11
11
11
11
11
11
11
11
11
10
10
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA, ELISA (Detection)
912
892
794
489
398
315
220
176
132
122
93
67
48
42
26
26
24
17
17
12
10
9
9
7
6
6
6
5
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN2001463
$ 218.90
Plus shipping costs $45.00

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 77-233
(90), (68), (52), (48), (25), (25), (25), (19), (17), (15), (10), (10), (8), (7), (7), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Ferret
(1503), (553), (484), (225), (217), (198), (197), (62), (55), (42), (31), (18), (13), (13), (9), (7), (5), (4), (4), (4), (3), (3), (2), (1), (1), (1)
Host Mouse
(1150), (573), (178), (48), (11), (10), (8), (7), (5), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(192), (103), (83), (63), (61), (39), (33), (31), (28), (26), (26), (26), (26), (25), (25), (25), (24), (20), (13), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (10), (10), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application ELISA, ELISA (Detection)
(912), (892), (794), (489), (398), (315), (220), (176), (132), (122), (93), (67), (48), (42), (26), (26), (24), (17), (17), (12), (10), (9), (9), (7), (6), (6), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Specificity Ferret TNFA / TNF-alpha
Has cross-reactivity in ELISA with
Canine TNFα
No cross-reactivity in ELISA with
Human TNFα
Mouse TNFα
E.coli cell lysate
Cross-Reactivity Dog (Canine)
No Cross-Reactivity Human, Mouse (Murine), Escherichia coli (E. coli)
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Ferret TNFA / TNF-alpha (rF TNFA / TNF-alpha, ABIN2008788 A3FBF1, Val 77-Leu 233,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Ferret TNFA / TNF-alpha protein (ABIN2008788)
Clone 11
Isotype IgG1
Plasmids, Primers & others

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF (TNF Antibody Abstract)
Background Tumor necrosis factor alpha (TNFa), also known as cachectin and TNFSF1A, is the prototypic cytokine of the the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNFa is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNFa is primarily produced as a 26 kDa type II transmembrane protein arranged in stable homotrimers, and a 55 kDa soluble trimeric form of TNFa is released via proteolytic cleavage by the metalloprotease TACE/ ADAM17. Two receptors mediating the TNFa function have been identified, the ubiquitous TNFRI from which most signaling are derived, and the hematopoietic cell-restricted TNFRII. Upon the TNFa trimers binding, TNF receptors also form trimers, undergo a conformational change, and enable the adaptor protein TRADD to bind to the death domain, and thus initiates the signal pathways such as the NF-kB, Jak/STAT, and the MAPK pathways, as well as the death signaling. As a major mediator of apoptosis, inflammation and immunity, TNFa has been implicated in the pathogenesis of a variety of diseases including autoimmune diseases, insulin resistance, and cancer.
UniProt A3FBF1
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Ferret TNFα. The detection limit for Ferret TNFα is approximately 0.078 ng/well.
ELISA(Det): 0.1-1 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months