anti-Human Tumor Necrosis Factor antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Epitope
AA 180-230
51
44
27
27
24
22
19
17
14
11
11
10
10
8
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1261
458
355
144
136
119
118
62
54
41
29
17
14
12
9
7
6
4
4
4
3
2
1
1
1
1
Host
Rabbit
936
528
177
50
10
9
8
7
5
3
2
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
171
96
73
50
49
32
30
28
27
25
25
25
25
25
24
24
24
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
904
864
621
323
289
230
224
190
143
107
97
67
29
29
25
22
20
18
14
11
9
7
6
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
Options
Supplier
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Catalog No. ABIN677318
$ 318.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.833679 ABIN446837 ICC IF IHC IHC (ff) IHC (fro) IHC (p) WB Rabbit AA 100-200, Internal Region Log in to see Polyclonal 13
12.833679 ABIN153474 IHC (p) IHC ELISA WB Rabbit Log in to see Polyclonal 9
12.833679 ABIN2506723 ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal 0
12.833679 ABIN498271 IF IHC (p) WB Rabbit Log in to see Polyclonal 2
12.833679 ABIN305730 FACS IHC (fro) IHC (p) ELISA WB Mouse IgG1 Log in to see 52B83 0
1 ABIN782382 EIA IHC (p) WB Mouse IgG1 AA 1-234 Log in to see M1-C4 0
1 ABIN4360905 FACS IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see 52B83 3
1 ABIN4360908 FACS IHC IHC (fro) IHC (p) WB FITC Mouse IgG1 Log in to see 52B83 1
1 ABIN263068 ELISA FACS ICC IF IHC IHC (fro) IHC (p) Mouse IgG1 kappa Log in to see MAb11 1
1 ABIN722481 IF (p) IHC (p) WB Mouse IgG Log in to see 1F6 1
1 ABIN263070 ICC FACS IF IHC (p) FITC Mouse IgG1 kappa Log in to see MAb11 1
1 ABIN959235 IHC (p) Mouse IgG1 Log in to see 2C8 0
1 ABIN563205 IHC (p) ELISA WB Mouse IgG1 kappa AA 1-233, full length Log in to see M1-C4 1
1 ABIN3187695 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal 0
1 ABIN573983 ELISpot IHC (p) ELISA Mouse IgG1, kappa Log in to see MAb1 0
1 ABIN790510 IHC (p) ELISA WB Mouse IgG1, kappa AA 1-234 Log in to see M 1 C4 0
1 ABIN1492935 IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN3201314 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 77-233 Log in to see 0
1 ABIN465605 ELISA IHC (p) Neut Mouse IgG1 Log in to see 0
1 ABIN5556639 EIA Func IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal 0

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 180-230
(51), (44), (27), (27), (24), (22), (19), (17), (14), (11), (11), (10), (10), (8), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1261), (458), (355), (144), (136), (119), (118), (62), (54), (41), (29), (17), (14), (12), (9), (7), (6), (4), (4), (4), (3), (2), (1), (1), (1), (1)
Host Rabbit
(936), (528), (177), (50), (10), (9), (8), (7), (5), (3), (2)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(171), (96), (73), (50), (49), (32), (30), (28), (27), (25), (25), (25), (25), (25), (24), (24), (24), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(904), (864), (621), (323), (289), (230), (224), (190), (143), (107), (97), (67), (29), (29), (25), (22), (20), (18), (14), (11), (9), (7), (6), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 12 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN677318) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from mouse TNF-alpha
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN677318) Images back to top
Antigen
Alternative Name Tnf alpha (TNF Antibody Abstract)
Background

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Subcellular location: Extracellular

Synonyms: DIF, Tnfa, TNF-a, TNFSF2, Tnfsf1a, TNFalpha, TNF-alpha, Tumor necrosis factor, Cachectin, Tumor necrosis factor ligand superfamily member 2, Tnf

Gene ID 21926
UniProt P06804
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN677318) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN677318) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-Tumor Necrosis Factor antibody (ABIN677318)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Dong, Zhou, Ji, Pan, Zheng: "AG1296 enhances plaque stability via inhibiting inflammatory responses and decreasing MMP-2 and MMP-9 expression in ApoE-/- mice." in: Biochemical and biophysical research communications, Vol. 489, Issue 4, pp. 426-431, 2017 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Han, Wang, Qin, Xia, Wu: "LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4." in: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 83, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Wang, Tang, Shen, Chen, Chen, Luo, Ge: "Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation." in: Cytokine, Vol. 99, pp. 275-280, 2017 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Shi, Yu, Liu, Liu, Zhang, Yang, Chai, Lou, Gao, Zhu: "Panax notoginseng saponins administration modulates pro- /anti-inflammatory factor expression and improves neurologic outcome following permanent MCAO in rats." in: Metabolic brain disease, Vol. 32, Issue 1, pp. 221-233, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rat (Rattus)). Further details: Sample: brain

Ji, Wang, Wang, Chen, Li: "Protective effect of Xuebijing injection against acute lung injury induced by left ventricular ischemia/reperfusion in rabbits." in: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 51-58, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rabbit). Further details: Sample: lung

Du, Niu, Wang, Zhao, Kong, Zhang, Nan: "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." in: Molecular medicine reports, Vol. 13, Issue 3, pp. 2159-66, 2016 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)). Further details: Sample: liver

Wang, Li, Wei, Xu, Chen, Song, Lu, Wang: "Targeted knockout of TNF?? by injection of lentivirus?mediated siRNA into the subacromial bursa for the treatment of subacromial bursitis in rats." in: Molecular medicine reports, Vol. 12, Issue 3, pp. 4389-95, 2015

Sun, Zeng, Cao, Song, Zhang, Liu: "Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue." in: BioMed research international, Vol. 2015, pp. 919401, 2015 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Xu, Zhao, Lin, Wang, Hu, Jiang: "Role of Dectin-1 in the innate immune response of rat corneal epithelial cells to Aspergillus fumigatus." in: BMC ophthalmology, Vol. 15, pp. 126, 2015 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rat (Rattus)).

Morishita, Oku, Horie, Tonari, Kida, Okubo, Sugiyama, Takai, Hara, Ikeda: "Systemic simvastatin rescues retinal ganglion cells from optic nerve injury possibly through suppression of astroglial NF-?B activation." in: PLoS ONE, Vol. 9, Issue 1, pp. e84387, 2014 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Rat (Rattus)).

Wang, Hu, Wang, Gu, Gao, Cao, Zheng: "5-Aminolevulinic acid-mediated sonodynamic therapy reverses macrophage and dendritic cell passivity in murine melanoma xenografts." in: Ultrasound in medicine & biology, Vol. 40, Issue 9, pp. 2125-33, 2014 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Wang, Zhang, Gu, Ding, Fan, Zhu, Shi, Hong, Kong: "Morin reduces hepatic inflammation-associated lipid accumulation in high fructose-fed rats via inhibiting sphingosine kinase 1/sphingosine 1-phosphate signaling pathway." in: Biochemical pharmacology, Vol. 86, Issue 12, pp. 1791-804, 2013 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)). Further details: Sample: liver

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN677318) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Antigen: 0.2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:...
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha P...
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Formalin-fixed and paraffin embedded rat hippocampus labeled with Rabbit Anti-TNF alp...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha P...
Western Blotting (WB) image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated at 1:300...
'Independent Validation' Badge
Lot Number 140113
Method validated Immunofluorescence
Positive Control Normal mouse lung tissue
Negative Control Normal mouse adipose tissue
Notes Fluorescent signal is detected in positive control, but not in negative control or isotype controls.
Primary Antibody
  • Antibody: Tumor Necrosis Factor (TNF)
  • Catalog number: ABIN677318
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 140113
Isotype Control Antibody
Secondary Antibody
  • Antibody: Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (Alexa 647)
  • Catalog number: Log in to see
  • Supplier: Log in to see
  • Lot number: Not available
Controls
  • Positive control: FFPE normal mouse lung tissue
  • Negative Control: FFPE normal mouse fat tissue
  • Isotype antibody control: tissue sections treated with Rabbit IgG Isotype at 1ug/ul
  • Secondary antibody only control: positive and negative control tissue sections treated with Goat anti-Rabbit Alexa 647 secondary antibody only. Any staining observed is due to non-specific binding of secondary antibody.
Protocol
  • Paraffin embedded positive and negative control tissue sections were deparaffinized and underwent antigen retrieval using 10 mM sodium citrate pH 6.0 in a pressure cooker for 10 min.
  • The tissue sections were incubated with 1 mg/mL sodium borohydride for 10 min at room temperature to block autofluorescent background signal. The sections were then rinsed three times in TBS for 5 min each at RT.
  • Tissue sections were blocked in 1 X TBS / 5% normal donkey serum for 60 min at RT.
  • Tissue sections were incubated with primary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum overnight at 4°C.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Cells were incubated with secondary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum for 60 min at RT in dark.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Coverslips were mounted on slides with Prolong Gold anti-fade mounting media (Invitrogen).
  • IF stained tissue sections were imaged with a Zeiss LSM 510 Meta confocal microscope.
Experimental Notes Nothing to note.
Validation Images
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 1: confocal image of positive control (mouse lung) with anti-TNF alpha antibody
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 2: confocal image of negative control (mouse adipose) with anti-TNF alpha antib...
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 3: confocal image of positive control (mouse lung) with isotype control
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 4: confocal image of negative control (mouse adipose) with isotype control
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 5: confocal image of positive control (mouse lung) with secondary antibody only