anti-Monkey Tumor Necrosis Factor antibody for ELISA

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Reactivity
Guinea Pig, Human, Monkey, Mouse (Murine)
1510
551
483
227
219
200
199
62
55
41
31
18
13
12
9
7
6
4
4
4
3
3
2
1
1
1
Host
Mouse
1159
573
173
48
11
10
8
7
5
2
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
190
102
82
63
61
39
33
31
28
26
26
26
26
25
25
25
24
20
13
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
912
893
792
492
402
317
221
176
133
122
95
67
48
42
26
26
25
17
17
12
10
9
9
7
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN305730
$ 727.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN225260 ELISA WB Mouse IgG1, kappa Log in to see MAb1 0
1 ABIN600294 ELISA FACS IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see 52B83 0
1 ABIN199738 FACS Neut ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN598274 ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN603912 Neut ELISA Mouse IgG2a Log in to see 0
1 ABIN3200803 ELISA FACS Func ICC IHC (fro) PE Mouse IgG1 Log in to see MAb11 0
1 ABIN3200991 ELISA FACS Func ICC IHC (fro) APC Mouse IgG1 Log in to see MAb11 0
1 ABIN3200802 ELISA FACS Func ICC IHC (fro) FITC Mouse IgG1 Log in to see MAb11 0
1 ABIN3200801 ELISA FACS Func ICC IHC (fro) Mouse IgG1 Log in to see MAb11 0
1 ABIN3040095 ELISA FACS IHC Mouse IgG1 Log in to see 0
1 ABIN3040098 ELISA FACS IHC FITC Mouse IgG1 Log in to see 0
1 ABIN3040099 ELISA FACS IHC PE Mouse IgG1 Log in to see 0
1 ABIN3040097 ELISA FACS IHC APC Mouse IgG1 Log in to see 0
1 ABIN2380218 IC IHC ELISA WB Mouse Log in to see 13j92 0

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Guinea Pig, Human, Monkey, Mouse (Murine)
(1510), (551), (483), (227), (219), (200), (199), (62), (55), (41), (31), (18), (13), (12), (9), (7), (6), (4), (4), (4), (3), (3), (2), (1), (1), (1)
Host Mouse
(1159), (573), (173), (48), (11), (10), (8), (7), (5), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(190), (102), (82), (63), (61), (39), (33), (31), (28), (26), (26), (26), (26), (25), (25), (25), (24), (20), (13), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(912), (893), (792), (492), (402), (317), (221), (176), (133), (122), (95), (67), (48), (42), (26), (26), (25), (17), (17), (12), (10), (9), (9), (7), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Brand IHC-plus™
Specificity Recognizes human native and recombinant TNF-alpha. Reacts with soluble (17kD) and membrane (26kD) human TNF-alpha. Does not cross-react with TNF-beta, lymphotoxin and receptor bound TNF-alpha.
Purification Purified
Immunogen Human TNFa.
Clone 52B83
Isotype IgG1
Plasmids, Primers & others

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF Alpha (TNF Antibody Abstract)
Background Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2
Gene ID 7124
UniProt P01375
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Approved: ELISA, Flo, IHC, IHC-Fr, IHC-P (2.5 μg/mL), WB (1:5)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Non-specific background staining is observed in connective tissues.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, 0.02 % sodium azide, 0.1 % BSA.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN305730) Human Lung (formalin-fixed, paraffin-embedded) stained with TNF antibody ABIN305730 a...