anti-Mouse (Murine) Tumor Necrosis Factor antibody for Cytometry by Time of Flight

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Epitope
AA 80-235
92
68
51
49
25
25
25
19
17
15
10
10
8
7
7
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
1511
551
483
227
219
200
199
62
55
42
31
18
13
13
9
7
6
4
4
4
3
3
2
1
1
1
Host
Goat
1160
573
173
47
11
10
8
7
5
2
2
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
190
102
82
63
61
39
33
31
28
26
26
26
26
25
25
25
24
20
13
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
913
893
792
493
402
318
222
175
132
122
95
67
48
42
26
26
25
17
16
12
10
9
8
7
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN4900489
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2661299 CyTOF FACS IF IHC IP Biotin Goat IgG Log in to see Polyclonal 3
1 ABIN4360972 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706 0
1 ABIN4360960 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa Log in to see SPM543 0
1 ABIN4360966 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see P-T2 0
1 ABIN4360974 CyTOF FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM Log in to see TNF706-P-T2 0
1 ABIN4360962 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see 4C6-H8 0

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 80-235
(92), (68), (51), (49), (25), (25), (25), (19), (17), (15), (10), (10), (8), (7), (7), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(1511), (551), (483), (227), (219), (200), (199), (62), (55), (42), (31), (18), (13), (13), (9), (7), (6), (4), (4), (4), (3), (3), (2), (1), (1), (1)
Host Goat
(1160), (573), (173), (47), (11), (10), (8), (7), (5), (2), (2)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(190), (102), (82), (63), (61), (39), (33), (31), (28), (26), (26), (26), (26), (25), (25), (25), (24), (20), (13), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
(913), (893), (792), (493), (402), (318), (222), (175), (132), (122), (95), (67), (48), (42), (26), (26), (25), (17), (16), (12), (10), (9), (8), (7), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 53 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN4900489) Images
Specificity Detects mouse TNF-alpha in ELISAs and Western blots. In sandwich immunoassays, approximately 50 % cross-reactivity with recombinant rat TNF-alpha is observed and less than 1 % cross-reactivity with recombinant human TNF-alpha is observed.
Purification Immunogen affinity purified
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen E. coli-derived recombinant mouse TNF-alpha, Leu80-Leu235, Accession # P06804
Isotype IgG
Plasmids, Primers & others

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN4900489) Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Alternative Names: APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, tnfa, TNF-a, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Gene ID 7124
UniProt P06804
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN4900489) Images back to top
Application Notes Western Blot 0.1 μg/mL
Neutralization 0.1-0.4 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 0.2-0.8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN4900489) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-Tumor Necrosis Factor antibody (ABIN4900489)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Tsunekawa, Banno, Mizoguchi, Sugiyama, Tominaga, Onoue, Hagiwara, Ito, Iwama, Goto, Suga, Sugimura, Arima: "Deficiency of PTP1B Attenuates Hypothalamic Inflammation via Activation of the JAK2-STAT3 Pathway in Microglia." in: EBioMedicine, Vol. 16, pp. 172-183, 2017 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Chung, Chatzigeorgiou, Economopoulou, Garcia-Martin, Alexaki, Mitroulis, Nati, Gebler, Ziemssen, Goelz, Phieler, Lim, Karalis, Papayannopoulou, Blüher, Hajishengallis, Chavakis: "A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity." in: Nature immunology, Vol. 18, Issue 6, pp. 654-664, 2017 Method employed by authors: Neutralization (Neut) (Sample species: Mouse (Murine)).

Matsushita, Hassanein, Martinez-Clemente, Lazaro, French, Xie, Lai, Karin, Tsukamoto: "Gender difference in NASH susceptibility: Roles of hepatocyte Ikkβ and Sult1e1." in: PLoS ONE, Vol. 12, Issue 8, pp. e0181052, 2017 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Oh, Kwon, Jung: "Anti-inflammatory Effects of the Natural Compounds CortexPhellodendriandHumulus japonicuson Pelvic Inflammatory Disease in Mice." in: International journal of medical sciences, Vol. 14, Issue 8, pp. 729-734, 2017 Method employed by authors: ELISA (Capture) (ELISA (Capture)) (Sample species: Mouse (Murine)).

Lee, Kwon, Kim, Jung, Cho: "Interferon-gamma regulates inflammatory cell death by targeting necroptosis in experimental autoimmune arthritis." in: Scientific reports, Vol. 7, Issue 1, pp. 10133, 2017 Method employed by authors: ELISA (Capture) (ELISA (Capture)) (Sample species: Mouse (Murine)).

Lim, Yuzhalin, Gordon-Weeks, Muschel: "Tumor-infiltrating monocytes/macrophages promote tumor invasion and migration by upregulating S100A8 and S100A9 expression in cancer cells." in: Oncogene, Vol. 35, Issue 44, pp. 5735-5745, 2016 Method employed by authors: Neutralization (Neut), Neutralization (Neut) (Sample species: Mouse (Murine)).

Lambertsen, Østergaard, Clausen, Hansen, Stenvang, Thorsen, Meldgaard, Kristensen, Hansen, Sorensen, Finsen: "No effect of ablation of surfactant protein-D on acute cerebral infarction in mice." in: Journal of neuroinflammation, Vol. 11, pp. 123, 2015 Method employed by authors: Functional Studies (Func) (Sample species: Mouse (Murine)).

Ramkhelawon, Hennessy, Ménager, Ray, Sheedy, Hutchison, Wanschel, Oldebeken, Geoffrion, Spiro, Miller, McPherson, Rayner, Moore: "Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity." in: Nature medicine, Vol. 20, Issue 4, pp. 377-84, 2014 Method employed by authors: Biochemical Assay (BCA), Biochemical Assay (BCA) (Sample species: Mouse (Murine)).

Shikata, Sakaue, Nakashiro, Okazaki, Kurata, Okamura, Okura, Ryugo, Nakamura, Yasugi, Higashiyama, Izutani: "Pathophysiology of lung injury induced by common bile duct ligation in mice." in: PLoS ONE, Vol. 9, Issue 4, pp. e94550, 2014 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Ilett, Kottke, Donnelly, Thompson, Willmon, Diaz, Zaidi, Coffey, Selby, Harrington, Pandha, Melcher, Vile: "Cytokine conditioning enhances systemic delivery and therapy of an oncolytic virus." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 22, Issue 10, pp. 1851-63, 2014 Method employed by authors: Neutralization (Neut), Neutralization (Neut) (Sample species: Mouse (Murine)).

Clatworthy, Harford, Mathews, Smith: "FcγRIIb inhibits immune complex-induced VEGF-A production and intranodal lymphangiogenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 50, pp. 17971-6, 2014 Method employed by authors: Neutralization (Neut), Neutralization (Neut) (Sample species: Mouse (Murine)).

Ingham, Williams, Bate: "Glimepiride reduces CD14 expression and cytokine secretion from macrophages." in: Journal of neuroinflammation, Vol. 11, pp. 115, 2014 Method employed by authors: ELISA (Capture) (ELISA (Capture)) (Sample species: Mouse (Murine)).

Hillian, McMullen, Sebastian, Roychowdhury, Rowchowdhury, Kashyap, Schauer, Kirwan, Feldstein, Nagy: "Mice lacking C1q are protected from high fat diet-induced hepatic insulin resistance and impaired glucose homeostasis." in: The Journal of biological chemistry, Vol. 288, Issue 31, pp. 22565-75, 2013 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Li, Shi, Shu, Gao, Wu, Tang et al.: "Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of ..." in: The Journal of biological chemistry, Vol. 288, Issue 19, pp. 13610-9, 2013 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Junkins, MacNeil, Wu, McCormick, Lin: "Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 190, Issue 10, pp. 5178-86, 2013 Method employed by authors: ELISA (Capture) (ELISA (Capture)) (Sample species: Mouse (Murine)).

Kim, Feng, Ohkuri, Sauers, Cohn, Chai, Nelson, Bachmanov, Huang, Wang: "Defects in the peripheral taste structure and function in the MRL/lpr mouse model of autoimmune disease." in: PLoS ONE, Vol. 7, Issue 4, pp. e35588, 2012 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Zhang, Yin, Zhang, Sun, Yang, Zhang, Salzman, Wang, Liu, Vidyasagar, Zhang, Ju, Okunieff, Zhang: "Response patterns of cytokines/chemokines in two murine strains after irradiation." in: Cytokine, Vol. 58, Issue 2, pp. 169-77, 2012 Method employed by authors: Luminex Assay (LMNX), Luminex Assay (LMNX) (Sample species: Mouse (Murine)).

Fisher, Chen, Skitzki, Muhitch, Zhou, Appenheimer, Vardam, Weis, Passanese, Wang, Gollnick, Dewhirst, Rose-John, Repasky, Baumann, Evans: "IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells." in: The Journal of clinical investigation, Vol. 121, Issue 10, pp. 3846-59, 2011 Method employed by authors: In vivo Studies (in vivo), In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Wieland, van der Windt, Florquin, McKenzie, van der Poll: "ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis." in: Microbes and infection, Vol. 11, Issue 4, pp. 524-30, 2009 Method employed by authors: ELISA (ELISA), ELISA (ELISA) (Sample species: Mouse (Murine)).

Wu, Mehta, Chakrabarty, Booth, Duggan, Patel, Ballard, Coggeshall, Metcalf: "Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 9, pp. 5799-806, 2009 Method employed by authors: ELISA (ELISA), ELISA (ELISA) (Sample species: Mouse (Murine)).

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN4900489) back to top
Supplier Images
Neutralization (Neut) image for anti-Tumor Necrosis Factor (TNF) (AA 80-235) antibody (ABIN4900489) Cytotoxicity Induced by TNF-alpha and Neutralization by Mouse TNF-alpha Antibody. Rec...
Immunocytochemistry (ICC) image for anti-Tumor Necrosis Factor (TNF) (AA 80-235) antibody (ABIN4900489) TNF-alpha in Mouse T Cells. TNF-alpha was detected in immersion fixed activated mouse...
Immunocytochemistry (ICC) image for anti-Tumor Necrosis Factor (TNF) (AA 80-235) antibody (ABIN4900489) TNF-alpha in RAW 264.7 Mouse Cell Line. TNF-alpha was detected in immersion fixed RAW...