anti-Rabbit Tumor Necrosis Factor antibody for Immunohistochemistry (Frozen Sections)

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LOC103694380
  • tnfa
Epitope
AA 80-235
97
69
51
48
26
22
19
15
14
10
10
8
8
7
6
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Rabbit
1362
521
454
221
216
197
196
59
56
43
32
18
15
9
7
6
6
5
4
4
4
4
3
2
2
1
1
1
1
Host
Mouse
1044
547
135
52
11
11
8
7
5
2
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
181
92
68
54
49
24
22
22
20
20
18
18
18
18
18
18
18
17
11
11
11
11
11
11
11
11
11
11
11
11
11
10
10
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
810
809
745
466
398
259
195
162
131
114
97
66
48
42
27
26
25
17
11
9
9
9
8
7
6
6
6
5
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Options
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Catalog No. ABIN5662008
$ 203.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
-8.269841 ABIN4360908 FACS IHC IHC (fro) IHC (p) WB FITC Mouse IgG1 Log in to see 52B83 1
-9.769841 ABIN4360976 ELISA FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 488 Mouse IgG1 kappa Log in to see SPM543 0
-12.769841 ABIN4360943 FACS ICC IF IHC (fro) IHC (p) Mouse IgM kappa N-Term Log in to see TNF706 0
-12.769841 ABIN4361077 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgM kappa N-Term Log in to see TNF706 0
-12.769841 ABIN4361078 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgM kappa N-Term Log in to see TNF706 0
-12.769841 ABIN4361082 ELISA ICC IF IHC (fro) IHC (p) Biotin Mouse IgM kappa N-Term Log in to see TNF706 0
-12.769841 ABIN4361083 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgM kappa N-Term Log in to see TNF706 0
-12.769841 ABIN4361087 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgM kappa N-Term Log in to see TNF706 0
-12.769841 ABIN4361091 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgM kappa N-Term Log in to see TNF706 0
-12.769841 ABIN4361092 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgM kappa N-Term Log in to see TNF706 0
-12.769841 ABIN4361084 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgM kappa N-Term Log in to see TNF706 0
-12.769841 ABIN4361080 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgM kappa N-Term Log in to see TNF706 0
-12.769841 ABIN4361085 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgM kappa N-Term Log in to see TNF706 0
-12.769841 ABIN4361079 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgM kappa N-Term Log in to see TNF706 0
-12.769841 ABIN4361090 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgM kappa N-Term Log in to see TNF706 0
-12.769841 ABIN4360946 FACS ICC IF IHC (fro) IHC (p) Mouse IgM Log in to see TNF706-P-T2 0
-12.769841 ABIN4360958 FACS ICC IF IHC (fro) IHC (p) Mouse IgG1 kappa Log in to see SPM543 0
-12.769841 ABIN4360937 FACS ICC IF IHC (fro) IHC (p) Mouse IgM kappa Log in to see 4C6-H8 0
-12.769841 ABIN4360940 FACS ICC IF IHC (fro) IHC (p) Mouse IgM kappa Log in to see P-T2 0
-12.769841 ABIN4360977 ELISA FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 647 Mouse IgG1 kappa Log in to see SPM543 0

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 80-235
(97), (69), (51), (48), (26), (22), (19), (15), (14), (10), (10), (8), (8), (7), (6), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rabbit
(1362), (521), (454), (221), (216), (197), (196), (59), (56), (43), (32), (18), (15), (9), (7), (6), (6), (5), (4), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(1044), (547), (135), (52), (11), (11), (8), (7), (5), (2), (1)
Clonality
Monoclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(181), (92), (68), (54), (49), (24), (22), (22), (20), (20), (18), (18), (18), (18), (18), (18), (18), (17), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (10), (10), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(810), (809), (745), (466), (398), (259), (195), (162), (131), (114), (97), (66), (48), (42), (27), (26), (25), (17), (11), (9), (9), (9), (8), (7), (6), (6), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN5662008) Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- L RSASRALSDK PLAHVVANPQ VEGQLQWLSQ RANALLANGM KLTDNQLVVP ADGLYLIYSQ VLFSGQGCRS YVLLTHTVSR FAVSYPNKVN LLSAIKSPCH RETPEEAEPM AWYEPIYLGG VFQLEKGDRL STEVNQPEYL DLAESGQVYF GIIAL
Specificity It has been selected for its ability to recognize TNFa in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen TNFa (AA 80-235)
Isotype IgG
Plasmids, Primers & others

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN5662008) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor Alpha (TNFa) (TNF Antibody Abstract)
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN5662008) Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN5662008) Images back to top
Format Liquid
Concentration 500 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

References for anti-Tumor Necrosis Factor antibody (ABIN5662008)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Larrañaga-Vera, Lamuedra, Pérez-Baos, Prieto-Potin, Peña, Herrero-Beaumont, Largo: "Increased synovial lipodystrophy induced by high fat diet aggravates synovitis in experimental osteoarthritis." in: Arthritis research & therapy, Vol. 19, Issue 1, pp. 264, 2017

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN5662008) back to top
Supplier Images
Western Blotting (WB) image for anti-Tumor Necrosis Factor (TNF) (AA 80-235) antibody (ABIN5662008) anti-Tumor Necrosis Factor (TNF) (AA 80-235) antibody