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|Antigen||Tumor Necrosis Factor (TNF) Antibodies|
|Reactivity||Human, Mouse (Murine), Sheep (Ovine) Alternatives|
|Conjugate||This Tumor Necrosis Factor antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
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Product Details anti-Tumor Necrosis Factor AntibodyTarget Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN4360905) Images
|Specificity||This detects both natural and recombinant TNFa. It does not cross-react with TNF beta or lymphotoxin. Non-specific background staining is obesrved in connective tissues.|
|Purification||Protein A or G purified|
|Immunogen||The monoclonal antibody 52B83 reacts with tumor necrosis factor alpha (TNF-alpha).|
|Plasmids, Primers & others|
Target Details Tumor Necrosis FactorProduct Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN4360905) Images back to top
|Alternative Name||TNF-alpha (TNF Antibody Abstract)|
|Background||Gene Symbol: TNF|
|Pathways||NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus|
Application DetailsProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN4360905) Images back to top
|Application Notes||Western Blot 1:10-1:100, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha (17.4 kDa) is not biologically active.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN4360905) Images back to top
PBS with 0.1 % BSA
Buffer contains: 0.02 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
References for anti-Tumor Necrosis Factor antibody (ABIN4360905)Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
|Product cited in:||
Zhou, Robert, Kapoulea, Lei, Stagner, Jakobiec, Dohlman, Paschalis: "Sustained Subconjunctival Delivery of Infliximab Protects the Cornea and Retina Following Alkali Burn to the Eye." in: Investigative ophthalmology & visual science, Vol. 58, Issue 1, pp. 96-105, 2017 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Rabbit).
Azeez, Olopade, Laperchia, Andrioli, Scambi, Onwuka, Bentivoglio, Olopade: "Regional Myelin and Axon Damage and Neuroinflammation in the Adult Mouse Brain After Long-Term Postnatal Vanadium Exposure." in: Journal of neuropathology and experimental neurology, Vol. 75, Issue 9, pp. 843-54, 2016 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry
Li, Das, Tang, Han, Jaiswal, Ray: "Transmembrane TNF-α Facilitates HIV-1 Infection of Podocytes Cultured from Children with HIV-Associated Nephropathy." in: Journal of the American Society of Nephrology : JASN, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).