anti-Sheep (Ovine) Tumor Necrosis Factor antibody for Flow Cytometry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Reactivity
Human, Mouse (Murine), Sheep (Ovine)
1510
551
483
227
219
200
199
62
55
42
30
18
13
13
9
7
6
4
4
4
3
3
2
1
1
1
Host
Mouse
1159
573
173
48
11
10
8
7
5
2
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
190
102
82
63
61
39
33
31
28
26
26
26
26
25
25
25
24
20
13
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
913
893
792
492
402
317
221
176
133
122
95
67
48
42
26
26
25
17
17
12
10
9
9
7
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN4360905
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4360908 FACS IHC IHC (fro) IHC (p) WB FITC Mouse IgG1 Log in to see 52B83 1
1 ABIN598279 FACS ELISA Biotin Mouse IgG2a Log in to see 0
1 ABIN213085 ELISA FACS Biotin Mouse IgG2a Log in to see 0
1 ABIN2476868 FACS IHC (fro) WB Mouse IgG2a Log in to see VPM61 4
1 ABIN4361152 CyTOF FACS IHC WB Mouse IgG1 Log in to see 52B83 0

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human, Mouse (Murine), Sheep (Ovine)
(1510), (551), (483), (227), (219), (200), (199), (62), (55), (42), (30), (18), (13), (13), (9), (7), (6), (4), (4), (4), (3), (3), (2), (1), (1), (1)
Host Mouse
(1159), (573), (173), (48), (11), (10), (8), (7), (5), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(190), (102), (82), (63), (61), (39), (33), (31), (28), (26), (26), (26), (26), (25), (25), (25), (24), (20), (13), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(913), (893), (792), (492), (402), (317), (221), (176), (133), (122), (95), (67), (48), (42), (26), (26), (25), (17), (17), (12), (10), (9), (9), (7), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN4360905) Images
Specificity This detects both natural and recombinant TNFa. It does not cross-react with TNF beta or lymphotoxin. Non-specific background staining is obesrved in connective tissues.
Purification Protein A or G purified
Immunogen The monoclonal antibody 52B83 reacts with tumor necrosis factor alpha (TNF-alpha).
Clone 52B83
Isotype IgG1
Plasmids, Primers & others

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN4360905) Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Gene Symbol: TNF
Gene ID 7124
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN4360905) Images back to top
Application Notes Western Blot 1:10-1:100, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha (17.4 kDa) is not biologically active.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN4360905) Images back to top
Format Liquid
Concentration 0.1 mg/mL
Buffer PBS with 0.1 % BSA
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-Tumor Necrosis Factor antibody (ABIN4360905)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Zhou, Robert, Kapoulea, Lei, Stagner, Jakobiec, Dohlman, Paschalis: "Sustained Subconjunctival Delivery of Infliximab Protects the Cornea and Retina Following Alkali Burn to the Eye." in: Investigative ophthalmology & visual science, Vol. 58, Issue 1, pp. 96-105, 2017 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Rabbit).

Azeez, Olopade, Laperchia, Andrioli, Scambi, Onwuka, Bentivoglio, Olopade: "Regional Myelin and Axon Damage and Neuroinflammation in the Adult Mouse Brain After Long-Term Postnatal Vanadium Exposure." in: Journal of neuropathology and experimental neurology, Vol. 75, Issue 9, pp. 843-54, 2016 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Li, Das, Tang, Han, Jaiswal, Ray: "Transmembrane TNF-α Facilitates HIV-1 Infection of Podocytes Cultured from Children with HIV-Associated Nephropathy." in: Journal of the American Society of Nephrology : JASN, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).