anti-Human High Mobility Group AT-Hook 1 antibody for Immunohistochemistry

Recommended High Mobility Group AT-Hook 1 Antibody (supplied by: Log in to see )

Antigen
High Mobility Group AT-Hook 1 (HMGA1) Antibodies
  • HMG-R
  • HMGA1A
  • HMGIY
  • Hmgi
  • Hmgiy
  • hmga1
  • hmgiy
  • wu:fb12b06
  • wu:fb18f11
  • wu:fc19f01
  • wu:fi38b05
  • zgc:85677
  • AL023995
  • Hmga1a
  • Hmga1b
  • Hmgy
  • HMG-I(Y)
  • HMG-Y
  • HMGA1
  • high mobility group AT-hook 1
  • high mobility group protein HMG-I/HMG-Y
  • high mobility group AT-hook 1a
  • HMGA1
  • Hmga1
  • TSC_c24220
  • hmga1a
  • LOC705105
Epitope
Internal Region
18
18
15
12
11
8
6
5
5
5
2
2
2
1
1
1
Reactivity
Human
125
67
67
31
25
15
9
8
6
6
6
3
2
1
1
1
1
1
Host
Goat
90
43
15
3
Clonality
Polyclonal
Conjugate
Un-conjugated
13
9
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC)
109
58
52
25
14
13
12
8
7
7
5
1
1
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Catalog No. ABIN185443
$ 429.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.25679 ABIN571040 ELISA IHC WB Goat Internal Region Log in to see Polyclonal 0
13.25679 ABIN3017095 IHC WB Rabbit Log in to see Polyclonal 0
13.132549 ABIN364402 ELISA IHC IHC (p) Goat AA 12-23 Log in to see Polyclonal 0
13.132549 ABIN490021 ELISA IHC IHC (p) WB Goat AA 9-21 Log in to see Polyclonal 0
13.132549 ABIN5920882 ELISA IHC WB Goat AA 9-21 Log in to see Polyclonal 0
10.132549 ABIN294187 IHC IHC (p) WB Rabbit AA 1-50 Log in to see Polyclonal 0
10.132549 ABIN6568818 IHC WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN1078142 ICC IHC WB Rabbit IgG AA 3-107 Log in to see Polyclonal 0
10 ABIN2932918 IF/ICC IHC IP WB Rabbit AA 3-107 Log in to see Polyclonal 0
7.1325493 ABIN2621955 ELISA IHC IHC (p) Rabbit IgG AA 1-53 Log in to see Polyclonal 0
7.1325493 ABIN2465818 IHC ELISA Goat Internal Region Log in to see Polyclonal 0
7.1325493 ABIN2470113 IHC WB Rabbit AA 1-50 Log in to see Polyclonal 0
7.1325493 ABIN5961095 ELISA IHC Rabbit IgG Log in to see Polyclonal 0
4.108079 ABIN4319317 IHC IHC (p) Rabbit IgG Log in to see Polyclonal 0
1 ABIN2283300 IHC ELISA FITC Goat Internal Region Log in to see Polyclonal 0
1 ABIN2283297 IHC ELISA Biotin Goat Internal Region Log in to see Polyclonal 0
1 ABIN2283296 IHC ELISA APC Goat Internal Region Log in to see Polyclonal 0
1 ABIN2283315 IHC ELISA PE Goat Internal Region Log in to see Polyclonal 0
1 ABIN2283313 IHC ELISA Alkaline Phosphatase (AP) Goat Internal Region Log in to see Polyclonal 0
1 ABIN2283312 IHC ELISA Goat Internal Region Log in to see Polyclonal 0

General

Antigen High Mobility Group AT-Hook 1 (HMGA1) Antibodies
Epitope Internal Region
(18), (18), (15), (12), (11), (8), (6), (5), (5), (5), (2), (2), (2), (1), (1), (1)
Reactivity Human
(125), (67), (67), (31), (25), (15), (9), (8), (6), (6), (6), (3), (2), (1), (1), (1), (1), (1)
Host Goat
(90), (43), (15), (3)
Clonality
Polyclonal
Conjugate Un-conjugated
(13), (9), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunohistochemistry (IHC)
(109), (58), (52), (25), (14), (13), (12), (8), (7), (7), (5), (1), (1)
Pubmed 2 references available
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Product details

Target Details Application Details Handling References Images
Purpose HMGA1
Blocking Peptide Blocking peptide for this product available: ABIN369400
Sequence LASKQEKDGT EK
Specificity This antibody is expected to recognise isoform a (also called HMG-I, NP_665906.1, NP_665908.1) and isoform b (also called HMG-Y, NP_002122.1, NP_665909.1, NP_665910.1, NP_665912.1).
Predicted Reactivity Human, Mouse, Rat, Dog, Pig, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence C-LASKQEKDGTEK, from the internal region of the protein sequence according to NP_665906.1, NP_665908.1, NP_002122.1, NP_665909.1, NP_665912.1, NP_665910.1.

Target Details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name HMGA1 (HMGA1 Antibody Abstract)
Background HMGA1, high mobility group AT-hook 1 , HGNC:5010, HMG-R, HMGIY, MGC12816, MGC4242, MGC4854 , OTTHUMP00000039618, high-mobility group (nonhistone chromosomal) protein isoforms I and Y, nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y, HMGA1A, OTTHUMP00000016223, OTTHUMP00000016224
Gene ID 3159
NCBI Accession NP_665906, NP_665908, NP_002122, NP_665909, NP_665912, NP_665910
Pathways Nuclear Hormone Receptor Binding, Autophagy

Application Details

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Application Notes

DS WB Results: Preliminary experiments gave an approx 50 kDa band in Human Testis and Ovary lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 11.7 kDa according to isoform a (also called HMG-I, NP_665906.1, NP_665908.1, NP_665911.1) and 10.7 kDa according to isoform b( (also called HMG-Y, NP_002122.1, NP_665909.1, NP_665910.1, NP_665912.1). The 50 kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

DS IHC Results: In paraffin embedded Human Placenta shows strong nuclear staining. Recommended concentration, 4-6 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions For Research Use only

Handling

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Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References

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Product cited in:

Di Cello, Shin, Harbom, Brayton: "Knockdown of HMGA1 inhibits human breast cancer cell growth and metastasis in immunodeficient mice." in: Biochemical and biophysical research communications, Vol. 434, Issue 1, pp. 70-4, 2013

Background publications

Chiappetta, Botti, Monaco, Pasquinelli, Pentimalli, Di Bonito, DAiuto, Fedele, Iuliano, Palmieri, Pierantoni, Giancotti, Fusco: "HMGA1 protein overexpression in human breast carcinomas: correlation with ErbB2 expression." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 22, pp. 7637-44, 2004