anti-Human High Mobility Group AT-Hook 1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended High Mobility Group AT-Hook 1 Antibody (supplied by: Log in to see )

Antigen
High Mobility Group AT-Hook 1 (HMGA1) Antibodies
  • HMG-R
  • HMGA1A
  • HMGIY
  • Hmgi
  • Hmgiy
  • hmga1
  • hmgiy
  • wu:fb12b06
  • wu:fb18f11
  • wu:fc19f01
  • wu:fi38b05
  • zgc:85677
  • AL023995
  • Hmga1a
  • Hmga1b
  • Hmgy
  • HMG-I(Y)
  • HMG-Y
  • HMGA1
  • high mobility group AT-hook 1
  • high mobility group protein HMG-I/HMG-Y
  • high mobility group AT-hook 1a
  • HMGA1
  • Hmga1
  • TSC_c24220
  • hmga1a
  • LOC705105
Epitope
Isoform A
19
18
15
13
11
8
6
5
5
5
2
2
2
2
1
1
1
Reactivity
Human
125
67
67
30
25
15
9
8
6
6
6
3
2
1
1
1
1
1
Host
Goat
90
42
15
3
Clonality
Polyclonal
Conjugate
Un-conjugated
13
9
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
110
57
51
23
14
13
12
8
7
7
5
1
1
Supplier
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Catalog No. ABIN4319316
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.951868 ABIN364402 ELISA IHC IHC (p) Goat AA 12-23 Log in to see Polyclonal 0
9.951868 ABIN4319317 IHC IHC (p) Rabbit IgG Log in to see Polyclonal 0
9.951868 ABIN294187 IHC IHC (p) WB Rabbit AA 1-50 Log in to see Polyclonal 0
9.951868 ABIN736626 IF (p) IHC (p) Rabbit IgG AA 60-107 Log in to see Polyclonal 1
9.951868 ABIN614899 EIA IHC (p) Goat Internal Region Log in to see Polyclonal 0
9.951868 ABIN615453 EIA IHC (p) WB Goat Internal Region Log in to see Polyclonal 0
9.951868 ABIN4319314 IHC (p) WB Rabbit Log in to see Polyclonal 0
9.951868 ABIN4319315 ELISA IHC (p) Goat Log in to see Polyclonal 0
1 ABIN5551871 EIA IHC (p) Goat Internal Region Log in to see Polyclonal 0
1 ABIN490021 ELISA IHC IHC (p) WB Goat AA 9-21 Log in to see Polyclonal 0
1 ABIN372421 IHC (p) WB Rabbit IgG AA 1-50, N-Term Log in to see Polyclonal 0
1 ABIN2621955 ELISA IHC IHC (p) Rabbit IgG AA 1-53 Log in to see Polyclonal 0
1 ABIN736635 IHC (p) HRP Rabbit IgG AA 60-107 Log in to see Polyclonal 0
1 ABIN1735497 IHC (p) ELISA WB Goat N-Term, Internal Region Log in to see Polyclonal 0
1 ABIN1735496 IHC (p) ELISA Goat Internal Region Log in to see Polyclonal 0
1 ABIN736628 IHC (p) Biotin Rabbit IgG AA 60-107 Log in to see Polyclonal 0
1 ABIN1735498 IHC (p) WB Rabbit IgG AA 1-50 Log in to see Polyclonal 0
1 ABIN2963034 IHC (p) ELISA Rabbit IgG AA 1-53 Log in to see Polyclonal 0
1 ABIN2767014 IHC (p) WB Rabbit AA 1-50 Log in to see Polyclonal 0
1 ABIN4319313 IHC (p) WB Rabbit Log in to see Polyclonal 0

General

Antigen High Mobility Group AT-Hook 1 (HMGA1) Antibodies
Epitope Isoform A
(19), (18), (15), (13), (11), (8), (6), (5), (5), (5), (2), (2), (2), (2), (1), (1), (1)
Reactivity Human
(125), (67), (67), (30), (25), (15), (9), (8), (6), (6), (6), (3), (2), (1), (1), (1), (1), (1)
Host Goat
(90), (42), (15), (3)
Clonality
Polyclonal
Conjugate Un-conjugated
(13), (9), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(110), (57), (51), (23), (14), (13), (12), (8), (7), (7), (5), (1), (1)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Specificity This antibody is expected to recognise isoform a (also called HMG-I, NP_665906.1, NP_665908.1) and isoform b (also called HMG-Y, NP_002122.1, NP_665909.1, NP_665910.1, NP_665912.1).
Purification Immunogen affinity purified
Immunogen Peptide with sequence C-LASKQEKDGTEK corresponding to internal region according to NP_665906.1, NP_665908.1, NP_002122.1, NP_665909.1, NP_665912.1, NP_665910.1.

Target Details

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Antigen
Alternative Name HMGA1 (HMGA1 Antibody Abstract)
Background Gene Symbol: HMGA1
Gene ID 3159
NCBI Accession NP_665906
Pathways Nuclear Hormone Receptor Binding, Autophagy

Application Details

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Application Notes Immunohistochemistry 4 - 6 μg/mL, Immunohistochemistry-Paraffin 1:10 - 1:500, Peptide ELISA Detection limit 1:32000WB: Preliminary experiments gave an approx. 50 kDa band in human testis and ovary lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 11.7 kDa band according to isoform a (also called HMG-I, NP_665906.1, NP_665908.1, NP_665911.1) and 10.7 kDa band according to isoform b( (also called HMG-Y, NP_002122.1, NP_665909.1, NP_665910.1, NP_665912.1). The 50 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human placenta shows strong nuclear staining.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.5 mg/mL Tris ( pH 7.3) and 0.5 % BSA
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References

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Product cited in:

Chiappetta, Botti, Monaco, Pasquinelli, Pentimalli, Di Bonito, DAiuto, Fedele, Iuliano, Palmieri, Pierantoni, Giancotti, Fusco: "HMGA1 protein overexpression in human breast carcinomas: correlation with ErbB2 expression." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 22, pp. 7637-44, 2004

Images

Product details Target Details Application Details Handling References back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-High Mobility Group AT-Hook 1 (HMGA1) (Isoform A) antibody (ABIN4319316) Immunohistochemistry-Paraffin: HMGA1 Antibody [ABIN4319316] - (4ug/ml) staining of pa...