anti-Rat (Rattus) High Mobility Group AT-Hook 1 antibody for Enzyme Immunoassay

Recommended High Mobility Group AT-Hook 1 Antibody (supplied by: Log in to see )

Antigen
High Mobility Group AT-Hook 1 (HMGA1) Antibodies
  • HMG-R
  • HMGA1A
  • HMGIY
  • Hmgi
  • Hmgiy
  • hmga1
  • hmgiy
  • wu:fb12b06
  • wu:fb18f11
  • wu:fc19f01
  • wu:fi38b05
  • zgc:85677
  • AL023995
  • Hmga1a
  • Hmga1b
  • Hmgy
  • HMG-I(Y)
  • HMG-Y
  • HMGA1
  • high mobility group AT-hook 1
  • high mobility group protein HMG-I/HMG-Y
  • high mobility group AT-hook 1a
  • HMGA1
  • Hmga1
  • TSC_c24220
  • hmga1a
  • LOC705105
Epitope
Internal Region
19
18
15
14
10
9
6
5
5
5
2
2
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
112
59
59
27
24
15
9
8
6
6
6
3
2
1
1
1
1
1
1
Host
Goat
76
40
13
3
Clonality
Polyclonal
Conjugate
Un-conjugated
10
8
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
94
67
37
23
14
13
7
7
4
4
1
1
1
Supplier
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Catalog No. ABIN5551871
$ 455.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5551861 EIA WB Goat AA 9-21, Internal Region Log in to see Polyclonal 0
1 ABIN614899 EIA IHC (p) Goat Internal Region Log in to see Polyclonal 0
1 ABIN615453 EIA IHC (p) WB Goat Internal Region Log in to see Polyclonal 0

General

Antigen High Mobility Group AT-Hook 1 (HMGA1) Antibodies
Epitope Internal Region
(19), (18), (15), (14), (10), (9), (6), (5), (5), (5), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(112), (59), (59), (27), (24), (15), (9), (8), (6), (6), (6), (3), (2), (1), (1), (1), (1), (1), (1)
Host Goat
(76), (40), (13), (3)
Clonality
Polyclonal
Conjugate Un-conjugated
(10), (8), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(94), (67), (37), (23), (14), (13), (7), (7), (4), (4), (1), (1), (1)
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Product details

Target Details Application Details Handling Images
Sequence c-LASKQEKDGT EK
Specificity This antibody detects HMGA1 (Internal).
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Dog, Pig, CowSpecies reactivity (tested):Human
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Immunogen Peptide from the internal region of the protein sequence according to NP_665906.1, NP_665908.1, NP_002122.1, NP_665909.1, NP_665912.1, NP_665910.1. Genename: HMGA1

Target Details

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Antigen
Alternative Name HMGA1 / HMGIY (HMGA1 Antibody Abstract)
Background HMGA1 (HMGI/Y) is a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. There are two splice isoforms of HMGA1 - the full length HMGA1a/HMGI and the shortened form HMGA1b/HMGY. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. HMGA1 is frequently acetylated and is found in the nucleus. Interacts with HIV-1 pre-integration complex.Synonyms: High mobility group AT-hook protein 1, High mobility group protein A1, High mobility group protein HMG-I/HMG-Y, High mobility group protein R
Gene ID 3159
NCBI Accession NP_002122
UniProt P17096
Pathways Nuclear Hormone Receptor Binding, Autophagy

Application Details

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Application Notes Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: Preliminary experiments gave an approx 50 kDa band in Human Testis andOvary lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot findan explanation in the literature for the band we observe given the calculated size of11.0 kDa according to isoform a (also called HMG-I, NP_665906.1, NP_665908.1,NP_665911.1) and 10.0 kDa according to isoform b( (also called HMG-Y, NP_002122.1,NP_665909.1, NP_665910.1, NP_665912.1). The 50 kDa band was successfully blocked byincubation with the immunizing peptide. We would appreciate any feedback from peoplein the field - have any results been reported with other antibodies/lysates? Have anyfurther splice variants/modified forms been reported?Immunohistochemistry: In paraffin embedded Human Placenta shows strong nuclearstaining. Recommended concentration, 4-6 μg/mL.
Restrictions For Research Use only

Handling

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Concentration 0.5 mg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.