anti-Rat (Rattus) High Mobility Group AT-Hook 1 antibody for Enzyme Immunoassay

Recommended High Mobility Group AT-Hook 1 Antibody (supplied by: Log in to see )

Antigen
High Mobility Group AT-Hook 1 (HMGA1) Antibodies
  • HMG-R
  • HMGA1A
  • HMGIY
  • Hmgi
  • Hmgiy
  • hmga1
  • hmgiy
  • wu:fb12b06
  • wu:fb18f11
  • wu:fc19f01
  • wu:fi38b05
  • zgc:85677
  • AL023995
  • Hmga1a
  • Hmga1b
  • Hmgy
  • HMG-I(Y)
  • HMG-Y
  • HMGA1
  • high mobility group AT-hook 1
  • high mobility group protein HMG-I/HMG-Y
  • high mobility group AT-hook 1a
  • HMGA1
  • Hmga1
  • TSC_c24220
  • hmga1a
  • LOC705105
Epitope
AA 9-21, Internal Region
18
18
15
14
10
8
6
5
5
4
2
2
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
124
64
64
29
24
15
9
8
6
6
6
3
2
1
1
1
1
1
1
Host
Goat
88
42
15
3
Clonality
Polyclonal
Conjugate
Un-conjugated
13
9
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
107
57
52
24
14
13
12
8
8
7
4
1
1
Supplier
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Catalog No. ABIN5551861
$ 462.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5551871 EIA IHC (p) Goat Internal Region Log in to see Polyclonal 0
1 ABIN614899 EIA IHC (p) Goat Internal Region Log in to see Polyclonal 0
1 ABIN615453 EIA IHC (p) WB Goat Internal Region Log in to see Polyclonal 0

General

Antigen High Mobility Group AT-Hook 1 (HMGA1) Antibodies
Epitope AA 9-21, Internal Region
(18), (18), (15), (14), (10), (8), (6), (5), (5), (4), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(124), (64), (64), (29), (24), (15), (9), (8), (6), (6), (6), (3), (2), (1), (1), (1), (1), (1), (1)
Host Goat
(88), (42), (15), (3)
Clonality
Polyclonal
Conjugate Un-conjugated
(13), (9), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(107), (57), (52), (24), (14), (13), (12), (8), (8), (7), (4), (1), (1)
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Product details

Target Details Application Details Handling Images
Sequence c-SQPLASKQEK DGT
Specificity This antibody is expected to recognize reported isoforms apha (NP_665906.1, NP_665908.1, NP_665911.1) and beta (NP_002122.1, NP_665910.1, NP_665912.1). This antibody is not expected to cross-react with HMGA2.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine, Pig.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Immunogen Peptide from the internal region (near N Terminus) of the protein sequence according to NP_665906.1, NP_002122.1. Genename: HMGA1

Target Details

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Antigen
Alternative Name HMGA1 / HMGIY (HMGA1 Antibody Abstract)
Background HMGA1 (HMGI/Y) is a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. There are two splice isoforms of HMGA1 - the full length HMGA1a/HMGI and the shortened form HMGA1b/HMGY. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. HMGA1 is frequently acetylated and is found in the nucleus. Interacts with HIV-1 pre-integration complex.Synonyms: High mobility group AT-hook protein 1, High mobility group protein A1, High mobility group protein HMG-I/HMG-Y, High mobility group protein R
Gene ID 3159
NCBI Accession NP_002122
UniProt P17096
Pathways Nuclear Hormone Receptor Binding, Autophagy

Application Details

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Application Notes Peptide ELISA: Detection Limit: 1/16000. Western blot: 0.5-1 μg/mL. Approx 24 kDa band observed in Human Pancreas (calculatedMW of 11.4 kDa/10.4 kDa according to NP_665906.1, NP_002122.1). The observed molecularweight corresponds to earlier findings with different antibodies from other commercialsources. This band was successfully blocked by incubation with the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

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Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.