anti-Mouse (Murine) Estrogen Receptor 2 (ER Beta) antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Estrogen Receptor 2 (ER Beta) Antibody (supplied by: Log in to see )

Antigen
Estrogen Receptor 2 (ER Beta) (ESR2) Antibodies
  • ER-BETA
  • ESR-BETA
  • ESRB
  • ESTRB
  • Erb
  • NR3A2
  • ER[b]1
  • zfER-beta1
  • zfER[b]1
  • erb2
  • CERB
  • ERB
  • erb
  • esrb
  • estrb
  • nr3a2
  • ERbeta
  • er-beta
  • esr-beta
  • ESR2
  • ERBETA
  • er
  • ER-beta
  • ER[b]
  • Estrb
  • ER[b]2
  • ER[b]a
  • zfER[b]2
  • zfER-beta2
  • Erb2
  • esr2
  • ERBeta2
  • estrogen receptor 2
  • estrogen receptor 2b
  • estrogen receptor beta 2
  • estrogen receptor 2 (beta)
  • estrogen receptor 2a
  • estrogen receptor beta
  • ESR2
  • esr2b
  • erb2
  • esr2
  • Esr2
  • esr2a
Epitope
C-Term
108
25
23
23
20
19
18
18
17
16
15
15
11
7
6
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
493
182
167
62
59
40
39
38
18
18
18
18
11
9
8
6
5
4
1
Host
Mouse
290
203
17
5
Clonality
Monoclonal
Conjugate
Un-conjugated
27
26
21
17
11
11
11
9
8
8
7
7
7
7
7
7
7
6
6
4
4
4
3
3
3
3
3
3
3
2
2
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
400
169
158
138
97
97
96
40
28
20
16
4
4
3
2
2
2
1
Supplier
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Catalog No. ABIN3024794
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.5 ABIN725210 FACS IF (p) IHC (p) WB Rabbit IgG AA 220-270 Log in to see Polyclonal 2
11.117387 ABIN214750 IHC IHC (p) WB Rabbit AA 82-131 Log in to see Polyclonal 0
10 ABIN3024798 FACS IF IHC (p) WB Mouse IgG2a C-Term Log in to see NR3Gb-1 0
4 ABIN781661 IHC (fro) IHC (p) Mouse IgG1 AA 512-530 Log in to see ERbeta-2D7 1
4 ABIN1387942 IF (p) IHC (p) WB Rabbit IgG pSer87 Log in to see Polyclonal 0
4 ABIN5960537 ELISA IF IHC (p) WB Rabbit IgG Ser105 Log in to see Polyclonal 0
1 ABIN725219 IHC (p) WB HRP Rabbit IgG AA 220-270 Log in to see Polyclonal 0
1 ABIN346611 GS ICC IF IHC IHC (fro) IHC (p) WB Rabbit AA 101-115 Log in to see Polyclonal 0
1 ABIN215126 IHC IHC (fro) IHC (p) WB Rabbit AA 512-530 Log in to see Polyclonal 0
1 ABIN215125 GS IHC IHC (fro) IHC (p) WB Rabbit AA 100-115 Log in to see Polyclonal 0
1 ABIN725212 IHC (p) WB Biotin Rabbit IgG AA 220-270 Log in to see Polyclonal 0
1 ABIN1415559 IHC (p) WB HRP Rabbit IgG pSer87 Log in to see Polyclonal 0
1 ABIN1397686 IHC (p) WB Biotin Rabbit IgG pSer87 Log in to see Polyclonal 0
1 ABIN1386285 IF (p) IHC (p) WB Rabbit IgG pSer105 Log in to see Polyclonal 0
1 ABIN2740736 ELISA FACS IF IHC IHC (p) IP WB Mouse IgG2a C-Term Log in to see ERb455 0
1 ABIN3024799 IHC (p) Mouse IgG2a C-Term Log in to see NR3Gb-1 0
1 ABIN3024795 IHC (p) Mouse IgG2a C-Term Log in to see ERb455 0
1 ABIN3024801 FACS IF IHC (p) WB Mouse IgG2a C-Term Log in to see NR3Gb-1 0
1 ABIN3024797 FACS IF IHC (p) WB Mouse IgG2a C-Term Log in to see ERb455 0

General

Antigen Estrogen Receptor 2 (ER Beta) (ESR2) Antibodies
Epitope C-Term
(108), (25), (23), (23), (20), (19), (18), (18), (17), (16), (15), (15), (11), (7), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
(493), (182), (167), (62), (59), (40), (39), (38), (18), (18), (18), (18), (11), (9), (8), (6), (5), (4), (1)
Host Mouse
(290), (203), (17), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(27), (26), (21), (17), (11), (11), (11), (9), (8), (8), (7), (7), (7), (7), (7), (7), (7), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(400), (169), (158), (138), (97), (97), (96), (40), (28), (20), (16), (4), (4), (3), (2), (2), (2), (1)
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Product details

Target Details Application Details Handling Images
Purification Protein G purified monoclonal antibody
Immunogen The C-terminus fragment of recombinant human Estrogen Receptor beta protein was used as the immunogen for this antibody.
Clone ERb455
Isotype IgG2a

Target Details

Product details Application Details Handling Images back to top
Antigen
Alternative Name Estrogen Receptor beta (ESR2 Antibody Abstract)
Background Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including alpha and beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER alpha and beta are differentially activated by various ligands. Ligand interaction triggers a cascade of events, including dissociation from heat shock proteins, recepter dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER alpha and beta may be regulated by distinct mechanisms even though they share many functional characteristics.
Gene ID 2100
Pathways Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling

Application Details

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Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Estrogen Receptor beta antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10e6 cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Restrictions For Research Use only

Handling

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Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the Estrogen Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

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Supplier Images
Flow Cytometry (FACS) image for anti-Estrogen Receptor 2 (ER Beta) (ESR2) (C-Term) antibody (ABIN3024794) FACS testing of BT474 cells: Black=cells alone; Gray=isotype control; Green=Estrogen...
Flow Cytometry (FACS) image for anti-Estrogen Receptor 2 (ER Beta) (ESR2) (C-Term) antibody (ABIN3024794) FACS testing of MCF-7 cells: Black=cells alone; Green=isotype control; Red=Estrogen ...
Immunohistochemistry (IHC) image for anti-Estrogen Receptor 2 (ER Beta) (ESR2) (C-Term) antibody (ABIN3024794) IHC testing of bladder carcinoma stained with Estrogen Receptor beta antibody (ERb455).
Immunohistochemistry (IHC) image for anti-Estrogen Receptor 2 (ER Beta) (ESR2) (C-Term) antibody (ABIN3024794) IHC testing of breast cancer stained with Estrogen Receptor beta antibody (ERb455).
Immunohistochemistry (IHC) image for anti-Estrogen Receptor 2 (ER Beta) (ESR2) (C-Term) antibody (ABIN3024794) IHC testing of gastric carcinoma stained with Estrogen Receptor beta antibody (ERb455).
Immunohistochemistry (IHC) image for anti-Estrogen Receptor 2 (ER Beta) (ESR2) (C-Term) antibody (ABIN3024794) IHC testing of ovarian cancer stained with Estrogen Receptor beta antibody (ERb455).