anti-Primate NR0B2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended NR0B2 Antibody

Antigen
Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) Antibodies
  • SHP
  • SHP1
  • SHP-1
  • Shp1
  • Shp
  • NR0B2-A
  • Nr0b2
  • SHP-A
  • gb:bc058069
  • nuclear receptor subfamily 0 group B member 2
  • nuclear receptor subfamily 0, group B, member 2
  • nuclear receptor subfamily 0, group B, member 2a
  • NR0B2
  • Nr0b2
  • nr0b2a
Reactivity
Hamster, Human, Monkey, Mouse (Murine), Primate, Rabbit
88
20
19
6
6
6
3
3
2
2
1
1
Host
Rabbit
70
16
4
Clonality
Polyclonal
Conjugate
This NR0B2 antibody is un-conjugated
4
3
3
2
2
2
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
72
38
32
26
15
13
13
6
3
1
1
1
1
Options

Available images

Catalog No. ABIN271164
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General

Antigen Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) Antibodies
Reactivity Hamster, Human, Monkey, Mouse (Murine), Primate, Rabbit
(88), (20), (19), (6), (6), (6), (3), (3), (2), (2), (1), (1)
Host Rabbit
(70), (16), (4)
Clonality
Polyclonal
Conjugate This NR0B2 antibody is un-conjugated
(4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(72), (38), (32), (26), (15), (13), (13), (6), (3), (1), (1), (1), (1)
Pubmed 2 references available
Supplier

Product Details anti-NR0B2 Antibody

Target Details NR0B2 Application Details Handling References for anti-NR0B2 antibody (ABIN271164) Images
Specificity Human NR0B2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Purification Immunogen affinity purified
Immunogen Synthetic 16 amino acid peptide from ligand-binding domain of human NR0B2.
Plasmids, Primers & others

Target Details NR0B2

Product Details anti-NR0B2 Antibody Application Details Handling References for anti-NR0B2 antibody (ABIN271164) Images back to top
Antigen
Alternative Name NR0B2 (NR0B2 Antibody Abstract)
Background Gene Symbol: NR0B2
Gene ID 8431
UniProt Q15466
Pathways Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway

Application Details

Product Details anti-NR0B2 Antibody Target Details NR0B2 Handling References for anti-NR0B2 antibody (ABIN271164) Images back to top
Application Notes Immunohistochemistry-Paraffin 4 μg/mL
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Nuclear Receptor SHP Antibody (NLS5411)Immunohistochemistry
1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
. Make 4 um sections and place on pre-cleaned and charged microscope slides.
. Heat in a tissue-drying oven for 45 minutes at 60 degrees Celcius.
. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each at RT.
. Rehydrate the tissues by washing the slides in 3 changes of 100 % alcohol for 3 minutes each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 3 minutes each at RT.
. Wash the slides in 1 change of 80 % alcohol for 3 minutes at RT.
. Rinse the slides in gentle running distilled water for 5 minutes at RT.
. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.
0) at 99-100 degrees Celciusfor 20 minutes.
. Remove the slides from the heat and let stand in buffer at RT for 20 minutes.
. Rinse the slides in 1X TBS-T for 1 minute at RT.**Do not allow the tissues to dry at any time during the staining procedure**
. Begin the immunostaining by applying a universal protein block for 20 minutes at RT.
. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase steptavidin for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase chromagen substrate for 30 minutes at RT.
. Rinse the slide in distilled water for 1 minute at RT.**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**
. Dehydrate the tissue by washing the slides in 2 changes of 80 % alcohol for 1 minute each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of 100 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of xylene for 1 minute each at RT.
. Apply cover slip.
Restrictions For Research Use only

Handling

Product Details anti-NR0B2 Antibody Target Details NR0B2 Application Details References for anti-NR0B2 antibody (ABIN271164) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-NR0B2 antibody (ABIN271164)

Product Details anti-NR0B2 Antibody Target Details NR0B2 Application Details Handling Images back to top
Product cited in:

He, Park, Zhang, Huang, Lu, Wang: "Epigenetic inhibition of nuclear receptor small heterodimer partner is associated with and regulates hepatocellular carcinoma growth." in: Gastroenterology, Vol. 134, Issue 3, pp. 793-802, 2008 Further details: Immunohistochemistry (Paraffin-embedded Sections)

Takahashi, Shibutani, Woo, Inoue, Fujimoto, Igarashi, Kanno, Hirose, Nishikawa: "Cellular distributions of molecules with altered expression specific to the tumor promotion process from the early stage in a rat two-stage hepatocarcinogenesis model." in: Carcinogenesis, Vol. 29, Issue 11, pp. 2218-26, 2008 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Images

Product Details anti-NR0B2 Antibody Target Details NR0B2 Application Details Handling References for anti-NR0B2 antibody (ABIN271164) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) antibody (ABIN271164) Immunohistochemistry-Paraffin: SHP/NR0B2/Nuclear Receptor SHP Antibody [NLS5411] - An...