anti-Sheep (Ovine) Nuclear Receptor Subfamily 1, Group D, Member 2 antibody for Western Blotting

Recommended Nuclear Receptor Subfamily 1, Group D, Member 2 Antibody (supplied by: Log in to see )

Antigen
Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2) Antibodies
  • BD73
  • EAR-1R
  • RVR
  • HZF-2
  • Rev-erb
  • REV-ERBB
  • NR1D2
  • rvr
  • bd73
  • hzf2
  • ear-1r
  • hs.37288
  • NR1D2-A
  • Reverbbeta-A
  • reverbb1
  • nuclear receptor subfamily 1 group D member 2
  • nuclear receptor subfamily 1, group D, member 2
  • nuclear receptor subfamily 1, group D, member 2a
  • NR1D2
  • Nr1d2
  • nr1d2
  • nr1d2a
Epitope
C-Term
11
11
10
9
8
7
5
5
4
4
3
2
2
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish (Danio rerio)
77
16
13
9
9
9
8
7
6
5
5
2
2
2
1
1
1
Host
Rabbit
65
15
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
Application
Western Blotting (WB)
54
30
29
23
5
3
3
2
1
1
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Catalog No. ABIN2780650
$ 249.00
Plus shipping costs $45.00

General

Antigen Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2) Antibodies
Epitope C-Term
(11), (11), (10), (9), (8), (7), (5), (5), (4), (4), (3), (2), (2), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish (Danio rerio)
(77), (16), (13), (9), (9), (9), (8), (7), (6), (5), (5), (2), (2), (2), (1), (1), (1)
Host Rabbit
(65), (15)
Clonality
Polyclonal
Conjugate Un-conjugated
(3), (3), (3), (2), (2), (2)
Application Western Blotting (WB)
(54), (30), (29), (23), (5), (3), (3), (2), (1), (1)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Sequence ETLIRALRTL IMKNHPNEAS IFTKLLLKLP DLRSLNNMHS EELLAFKVHP
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 92%, Zebrafish: 93%
Characteristics This is a rabbit polyclonal antibody against NR1D2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human NR1D2

Target Details

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Antigen
Alternative Name NR1D2 (NR1D2 Antibody Abstract)
Background NR1D2 can interact with NCOA5 coactivator, leading to a strong increase of transcription of target genes. NR1D2 also binds to the sequences 5'-AATGTAGGTCA-3' and 5'-ATAACTAGGTCA-3' and acts as a potent competitive transcriptional silencer and negative regulator of RORalpha mediated trans-activation. NR1D2 may play different roles in metabolism, inflammation, and circadian cycling in the organ-specific manner in homeostasis.
Alias Symbols: RVR, BD73, EAR-1R
Protein Interaction Partner: MID2, RBPMS, TRIM27, NOTCH2NL, KRTAP10-9, KRT40, KRTAP4-2, PRMT6, SKP1, UBC, SIN3B, KAT5, NCOR1, TAF9, TAF6, HDAC1, GTF2B, NCOA5, UBE2I, CUL1, NR1D1, RGN,
Protein Size: 579
Molecular Weight 65 kDa
Gene ID 9975
NCBI Accession NM_005126, NP_005117
UniProt Q14995
Pathways Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 579 AA

Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Background publications

Sauvé, McBroom, Gallant, Moraitis, Labrie, Giguère: "CIA, a novel estrogen receptor coactivator with a bifunctional nuclear receptor interacting determinant." in: Molecular and cellular biology, Vol. 21, Issue 1, pp. 343-53, 2001

Images

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Supplier Images
Western Blotting (WB) image for anti-Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2) (C-Term) antibody (ABIN2780650) WB Suggested Anti-NR1D2 Antibody Titration: 0.5ug/ml ELISA Titer: 1:62500 Positiv...