anti-Rabbit Nuclear Receptor Subfamily 1, Group H, Member 3 antibody for Western Blotting
Recommended Nuclear Receptor Subfamily 1, Group H, Member 3 Antibody (supplied by: Log in to see )
- Antigen
-
Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) Antibodies
show synonyms for this antigen- NR1H3
- LXR-a
- LXRA
- RLD-1
- AU018371
- LXR
- RLD1
- Unr1
- LXRalpha
- LXR-alpha
- im:7141239
- wu:fd59b01
- zgc:113339
- zgc:158542
- nuclear receptor subfamily 1 group H member 3
- nuclear receptor subfamily 1, group H, member 3
- NR1H3
- nr1h3
- Nr1h3
- Epitope
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C-Term
Alternatives - Reactivity
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Bat, Chicken, Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
Alternatives - Host
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Rabbit
Alternatives - Clonality
- Conjugate
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Un-conjugated
Alternatives - Application
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Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Alternatives - Options
- Supplier
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- Supplier Product No.
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Available images
Catalog No. ABIN6741573
$ 727.83
Plus shipping costs $45.00
Plus shipping costs $45.00
| Relevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
|---|---|---|---|---|---|---|---|---|---|---|
| 12.343901 | ABIN2780709 | WB | Rabbit | C-Term | Log in to see | Polyclonal | 1 | |||
| 4.8439007 | ABIN2779794 | WB | Rabbit | C-Term | Log in to see | Polyclonal | 0 | |||
| 4.8439007 | ABIN2780708 | WB | Rabbit | N-Term | Log in to see | Polyclonal | 1 | |||
| 4 | ABIN297261 | ELISA WB | Goat | AA 384-397 | Log in to see | Polyclonal | 0 | |||
| 4 | ABIN6748254 | WB | Rabbit | AA 356-405 | Log in to see | Polyclonal | 0 |
General |
|
|---|---|
| Antigen | Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) Antibodies |
| Epitope | C-Term Alternatives |
| Reactivity | Bat, Chicken, Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio) Alternatives |
| Host | Rabbit Alternatives |
| Clonality | |
| Conjugate | Un-conjugated Alternatives |
| Application |
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Alternatives
|
| Supplier | Log in to see |
Product detailsTarget Details Application Details Handling Images |
|
| Brand | IHC-plus™ |
| Specificity | Human NR1H3 / LXR Alpha |
| Predicted Reactivity | Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Goat, Pig, Guinea pig, Chicken, Zebrafish (100%). |
| Purification | Immunoaffinity purified |
| Immunogen |
Synthetic peptide from C-Terminus of human NR1H3 (Q13133, NP_005684). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Trout, Salmon, Stickleback, Zebrafish (100%). Type of Immunogen: Synthetic peptide |
| Isotype | IgG |
Target DetailsProduct details Application Details Handling Images back to top |
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| Antigen | |
| Alternative Name | NR1H3 / LXR Alpha (NR1H3 Antibody Abstract) |
| Background |
Name/Gene ID: NR1H3 Subfamily: NR1 Thyroid hormone-like Family: NHR Synonyms: NR1H3, Liver X receptor alpha, LXRA, LXR-a, Oxysterols receptor LXR-alpha, LXR alpha, Lxralpha, RLD-1 |
| Gene ID | 10062 |
| NCBI Accession | NP_005684 |
| UniProt | Q13133 |
| Pathways | Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Nuclear Hormone Receptor Binding, Cellular Response to Molecule of Bacterial Origin, Hepatitis C |
Application DetailsProduct details Target Details Handling Images back to top |
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| Application Notes |
Approved: IHC, IHC-P (5 μg/mL), WB Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. |
| Comment |
Target Species of Antibody: Human |
| Restrictions | For Research Use only |
HandlingProduct details Target Details Application Details Images back to top |
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| Format | Lyophilized |
| Reconstitution | Distilled water |
| Concentration | Lot specific |
| Buffer | Lyophilized from PBS with 2 % sucrose |
| Handling Advice | Avoid repeat freeze-thaw cycles. |
| Storage | 4 °C,-20 °C |
| Storage Comment |
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year) Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles. |
ImagesProduct details Target Details Application Details Handling back to top |
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| Supplier Images |
Human Colon (formalin-fixed, paraffin-embedded) stained with NR1H3 antibody ABIN46221...
Human Small Intestine (formalin-fixed, paraffin-embedded) stained with NR1H3 antibody...
Human Liver (formalin-fixed, paraffin-embedded) stained with NR1H3 antibody ABIN46221...
Anti-NR1H3 / LXR Alpha antibody IHC of human colon. Immunohistochemistry of formalin-...
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