anti-Yeast (Saccharomyces cerevisiae) Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) antibody for Flow Cytometry

Recommended Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) Antibody

Antigen
Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) Antibodies
  • GR
  • gcr
  • grl
  • xGR
  • gccr
  • gr
  • zgc:113038
  • NR3C1
  • GCL
  • LOC100302444
  • Gcr
  • Grl
  • Grl-1
  • Grl1
  • GRL
  • GCCR
  • GCR
  • NO
  • GR-A
  • nr3c1
  • nuclear receptor subfamily 3 group C member 1
  • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
  • nuclear receptor subfamily 3, group C, member 1
  • glucocorticoid receptor
  • NR3C1
  • nr3c1
  • LOC100302444
  • Nr3c1
Reactivity
Guinea Pig, Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Yeast (Saccharomyces cerevisiae)
291
151
135
8
7
6
5
5
4
4
3
1
1
1
Host
Mouse
250
57
5
Clonality
Monoclonal
Conjugate
Un-conjugated
23
17
10
7
6
6
6
6
6
6
6
6
3
3
3
3
3
1
1
Application
Blocking Peptide (BP), Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Neutralization (Neut), Western Blotting (WB)
226
120
105
63
53
39
35
29
27
17
4
3
2
1
1
Options

Available images

Catalog No. ABIN268501
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
-4.753898 ABIN4315957 FACS PE Mouse IgG2 BuGR2 0
-4.753898 ABIN4315966 FACS ICC IF IHC IHC (p) WB Alexa Fluor 647 Mouse IgG2 BuGR2 0
-13.073406 ABIN4315955 FACS ICC IF IHC IHC (p) WB DyLight 350 Mouse IgG2 BuGR2 0
-13.073406 ABIN4315956 FACS ICC IF IHC IHC (p) WB DyLight 405 Mouse IgG2 BuGR2 0
-13.073406 ABIN4315959 FACS APC Mouse IgG2 BuGR2 0
-13.073406 ABIN4315960 FACS ICC IF IHC IHC (p) WB DyLight 755 Mouse IgG2 BuGR2 0
-13.073406 ABIN4315961 FACS ICC IF IHC IHC (p) WB DyLight 488 Mouse IgG2 BuGR2 0
-13.073406 ABIN4315958 BP ChIP FACS GS ICC IF IHC IHC (p) IP Neut WB PerCP Mouse IgG2 BuGR2 0
-13.073406 ABIN4315964 FACS ICC IF IHC IHC (p) WB Alexa Fluor 405 Mouse IgG2 BuGR2 0
-13.073406 ABIN4315963 FACS ICC IF IHC IHC (p) WB DyLight 680 Mouse IgG2 BuGR2 0
-13.073406 ABIN4315965 FACS ICC IF IHC IHC (p) WB Alexa Fluor 488 Mouse IgG2 BuGR2 0
-13.073406 ABIN4315967 FACS ICC IF IHC IHC (p) WB Alexa Fluor 700 Mouse IgG2 BuGR2 0
-13.073406 ABIN5951921 FACS ICC IF IHC IHC (p) Janelia Fluor® 549 Mouse IgG2 BuGR2 0
-13.073406 ABIN5951922 FACS ICC IF IHC IHC (p) Janelia Fluor® 646 Mouse IgG2 BuGR2 0

General

Antigen Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) Antibodies
Reactivity Guinea Pig, Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Yeast (Saccharomyces cerevisiae)
(291), (151), (135), (8), (7), (6), (5), (5), (4), (4), (3), (1), (1), (1)
Host Mouse
(250), (57), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(23), (17), (10), (7), (6), (6), (6), (6), (6), (6), (6), (6), (3), (3), (3), (3), (3), (1), (1)
Application Blocking Peptide (BP), Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Neutralization (Neut), Western Blotting (WB)
(226), (120), (105), (63), (53), (39), (35), (29), (27), (17), (4), (3), (2), (1), (1)
Pubmed 8 references available
Supplier

Product details

Target Details Application Details Handling References Images
Specificity Using enzymatic digestion analysis, has been shown to react with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.
Purification Protein A purified
Immunogen Partially purified rat GR.
Clone BuGR2
Isotype IgG2

Target Details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name GR/NR3C1 (NR3C1 Antibody Abstract)
Background Gene Symbol: NR3C1
Gene ID 2908
Pathways Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Regulation of Carbohydrate Metabolic Process

Application Details

Product details Target Details Handling References Images back to top
Application Notes Western Blot 5 μg/mL, Chromatin Immunoprecipitation 1:10 - 1:500, Flow Cytometry 1:10 - 1:1000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 5 μg/mL, Gel Super Shift Assays 1:1- 1:100, Blocking/Neutralizing 50 - 200 Molar excessBlocking, ChIP, and ELISA usages were reported in scientific literature.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Format Lyophilized
Reconstitution Reconstitute with 0.1 mL sterilized water to desired concentration.
Buffer PBS ( pH 7.2)
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References

Product details Target Details Application Details Handling Images back to top
Product cited in:

Taves, Mittelstadt, Presman, Hager, Ashwell: "Single-Cell Resolution and Quantitation of Targeted Glucocorticoid Delivery in the Thymus." in: Cell reports, Vol. 26, Issue 13, pp. 3629-3642.e4, 2019 (Sample species: Mouse (Murine)).

Rué, Bañez-Coronel, Creus-Muncunill, Giralt, Alcalá-Vida, Mentxaka, Kagerbauer, Zomeño-Abellán, Aranda, Venturi, Pérez-Navarro, Estivill, Martí: "Targeting CAG repeat RNAs reduces Huntington's disease phenotype independently of huntingtin levels." in: The Journal of clinical investigation, Vol. 126, Issue 11, pp. 4319-4330, 2016

Wu, Pinello, Yissachar, Wischhusen, Yuan, Roberts: "Functionally distinct patterns of nucleosome remodeling at enhancers in glucocorticoid-treated acute lymphoblastic leukemia." in: Epigenetics & chromatin, Vol. 8, pp. 53, 2015 Further details: Chromatin Immunoprecipitation

Schoenfelder, Hiemke, Schmitt: "Behavioural consequences of p-glycoprotein deficiency in mice, with special focus on stress-related mechanisms." in: Journal of neuroendocrinology, Vol. 24, Issue 5, pp. 809-17, 2012 (Sample species: Mouse (Murine)).

Viegas, Vicent, Barañao, Beato, Pecci: "Steroid hormones induce bcl-X gene expression through direct activation of distal promoter P4." in: The Journal of biological chemistry, Vol. 279, Issue 11, pp. 9831-9, 2004

Harrell, Murphy, Morishima, Chen, Mansfield, Galigniana, Pratt: "Evidence for glucocorticoid receptor transport on microtubules by dynein." in: The Journal of biological chemistry, Vol. 279, Issue 52, pp. 54647-54, 2004

Lambert, Nordeen: "CBP recruitment and histone acetylation in differential gene induction by glucocorticoids and progestins." in: Molecular endocrinology (Baltimore, Md.), Vol. 17, Issue 6, pp. 1085-94, 2003

Hutchison, Dittmar, Pratt et al.: "All of the factors required for assembly of the glucocorticoid receptor into a functional heterocomplex with heat shock protein 90 are preassociated in a self-sufficient protein folding structure, a ..." in: The Journal of biological chemistry, Vol. 269, Issue 45, pp. 27894-9, 1994

Images

Product details Target Details Application Details Handling References back to top
Supplier Images
Immunofluorescence (IF) image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody (ABIN268501) Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [ABIN268501] - Anal...
Western Blotting (WB) image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody (ABIN268501) Western Blot: GR/NR3C1 Antibody (BuGR2) [ABIN268501] - Analysis of glucocorticoid rec...
Immunofluorescence (IF) image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody (ABIN268501) Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [ABIN268501] - Anal...
Immunofluorescence (IF) image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody (ABIN268501) Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [ABIN268501] - Anal...
Flow Cytometry (FACS) image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody (ABIN268501) Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [ABIN268501] - Analysis of Glucocorticoid R...
Flow Cytometry (FACS) image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody (ABIN268501) Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [ABIN268501] - Analysis of Glucocorticoid R...
Flow Cytometry (FACS) image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody (ABIN268501) Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [ABIN268501] - Analysis of GR in Jurkat cel...
Flow Cytometry (FACS) image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody (ABIN268501) Flow Cytometry: GR/NR3C1 Antibody (BuGR2) - Using the Alexa Fluor 647 direct conjuga...
Flow Cytometry (FACS) image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody (ABIN268501) Flow Cytometry: GR/NR3C1 Antibody (BuGR2) - An intracellular stain was performed on ...