anti-Human RORB antibody for Immunohistochemistry

Recommended RORB Antibody (supplied by: Log in to see )

Antigen
RAR-Related Orphan Receptor B (RORB) Antibodies
  • RORB
  • Nr1f2
  • LOC100226267
  • NR1F2
  • ROR-BETA
  • RZR-BETA
  • RZRB
  • bA133M9.1
  • nr1f2
  • rzrb
  • sb:eu943
  • RZR-beta
  • Rorbeta
  • RAR related orphan receptor B
  • RAR-related orphan receptor B
  • RAR-related orphan receptor beta
  • RORB
  • Rorb
  • rorb
Epitope
Hinge Region
11
10
9
7
7
6
4
3
3
3
2
1
1
1
1
1
Reactivity
Bat, Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
94
35
33
9
9
9
9
9
6
6
6
6
4
3
3
2
2
1
Host
Rabbit
72
22
Clonality
Polyclonal
Conjugate
This RORB antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
60
40
32
22
13
2
Supplier
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Catalog No. ABIN1049297
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.289492 ABIN1049295 IHC IHC (p) Rabbit Ligand Binding Domain Log in to see Polyclonal 0
9.289492 ABIN1049296 ELISA IHC IHC (p) Rabbit Hinge Region Log in to see Polyclonal 0
9.289492 ABIN4350953 IHC WB Mouse IgG1 Log in to see 1G1 0
9.289492 ABIN2427097 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
9.289492 ABIN2433709 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN4350951 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2353280 IHC ELISA WB Rabbit IgG C-Term, AA 328-358 Log in to see Polyclonal 0
1 ABIN1931447 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 329-358 Log in to see Polyclonal 0
1 ABIN1931452 IHC ELISA WB HRP Rabbit IgG AA 329-358 Log in to see Polyclonal 0
1 ABIN1931448 IHC ELISA WB APC Rabbit IgG AA 329-358 Log in to see Polyclonal 0
1 ABIN1931449 IHC ELISA WB Biotin Rabbit IgG AA 329-358 Log in to see Polyclonal 0
1 ABIN1931450 IHC ELISA WB FITC Rabbit IgG AA 329-358 Log in to see Polyclonal 0
1 ABIN1931451 IHC ELISA WB PE Rabbit IgG AA 329-358 Log in to see Polyclonal 0
1 ABIN1810346 IHC IHC (p) WB Rabbit AA 329-358 Log in to see Polyclonal 0
1 ABIN2579380 ELISA IHC WB Rabbit IgG AA 329-358 Log in to see Polyclonal 0
1 ABIN2353277 IHC ELISA WB HRP Rabbit IgG C-Term, AA 328-358 Log in to see Polyclonal 0
1 ABIN2353276 IHC ELISA WB FITC Rabbit IgG C-Term, AA 328-358 Log in to see Polyclonal 0
1 ABIN2353274 IHC ELISA WB APC Rabbit IgG C-Term, AA 328-358 Log in to see Polyclonal 0
1 ABIN2353278 IHC ELISA WB PE Rabbit IgG C-Term, AA 328-358 Log in to see Polyclonal 0
1 ABIN2353273 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term, AA 328-358 Log in to see Polyclonal 0

General

Antigen RAR-Related Orphan Receptor B (RORB) Antibodies
Epitope Hinge Region
(11), (10), (9), (7), (7), (6), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Reactivity Bat, Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
(94), (35), (33), (9), (9), (9), (9), (9), (6), (6), (6), (6), (4), (3), (3), (2), (2), (1)
Host Rabbit
(72), (22)
Clonality
Polyclonal
Conjugate This RORB antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(60), (40), (32), (22), (13), (2)
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Product Details anti-RORB Antibody

Target Details RORB Application Details Handling Images
Brand IHC-plus™
Specificity Human ROR Beta. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Hamster, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Chicken, Platypus, Lizard (100%) Elephant, Xenopus (94%) Zebrafish (83%).
Purification Immunoaffinity purified
Immunogen Synthetic 18 amino acid peptide from hinge domain of human ROR Beta. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Hamster, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Chicken, Platypus, Lizard (100%), Elephant, Xenopus (94%), Zebrafish (83%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details RORB

Product Details anti-RORB Antibody Application Details Handling Images back to top
Antigen
Alternative Name RORB / ROR beta (RORB Antibody Abstract)
Background Name/Gene ID: RORB
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: RORB, BA133M9.1, Nuclear receptor ROR-beta, NR1F2, ROR Beta, ROR-BETA, Rorbeta, RZR-BETA, Nuclear receptor RZR-beta, RAR-related orphan receptor B, RZRB
Gene ID 6096
Pathways Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway

Application Details

Product Details anti-RORB Antibody Target Details RORB Handling Images back to top
Application Notes Approved: IHC, IHC-P (20 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-RORB Antibody Target Details RORB Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-RORB Antibody Target Details RORB Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-RAR-Related Orphan Receptor B (RORB) (Hinge Region) antibody (ABIN1049297) Anti-ROR Beta antibody ABIN1049297 IHC staining of human brain, cortex. Immunohistoc...