anti-Rat (Rattus) RORB antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended RORB Antibody (supplied by: Log in to see )

Antigen
RAR-Related Orphan Receptor B (RORB) Antibodies
  • RORB
  • Nr1f2
  • LOC100226267
  • NR1F2
  • ROR-BETA
  • RZR-BETA
  • RZRB
  • bA133M9.1
  • nr1f2
  • rzrb
  • sb:eu943
  • RZR-beta
  • Rorbeta
  • RAR related orphan receptor B
  • RAR-related orphan receptor B
  • RAR-related orphan receptor beta
  • RORB
  • Rorb
  • rorb
Reactivity
Bat, Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Primate, Rabbit, Rat (Rattus), Sheep (Ovine)
92
33
31
9
9
9
9
9
6
6
6
6
4
3
3
2
1
1
Host
Rabbit
70
22
Clonality
Polyclonal
Conjugate
This RORB antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
56
36
24
22
13
Supplier
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Available images

Catalog No. ABIN270818
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.83519 ABIN4350949 IHC (p) Rabbit Log in to see Polyclonal 0
1 ABIN1049295 IHC (p) Rabbit Ligand Binding Domain Log in to see Polyclonal 0
1 ABIN1049297 IHC (p) Rabbit Hinge Region Log in to see Polyclonal 0
1 ABIN715871 IF (p) IHC (p) Rabbit IgG Log in to see Polyclonal 0
1 ABIN715880 IHC (p) HRP Rabbit IgG Log in to see Polyclonal 0
1 ABIN715873 IHC (p) Biotin Rabbit IgG Log in to see Polyclonal 0

General

Antigen RAR-Related Orphan Receptor B (RORB) Antibodies
Reactivity Bat, Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Primate, Rabbit, Rat (Rattus), Sheep (Ovine)
(92), (33), (31), (9), (9), (9), (9), (9), (6), (6), (6), (6), (4), (3), (3), (2), (1), (1)
Host Rabbit
(70), (22)
Clonality
Polyclonal
Conjugate This RORB antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(56), (36), (24), (22), (13)
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Product Details anti-RORB Antibody

Target Details RORB Application Details Handling Images
Specificity Human ROR Beta. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Purification Immunogen affinity purified
Immunogen Synthetic 20 amino acid peptide from ligand-binding domain of human ROR Beta.

Target Details RORB

Product Details anti-RORB Antibody Application Details Handling Images back to top
Antigen
Alternative Name ROR beta (RORB Antibody Abstract)
Background Gene Symbol: RORB
Gene ID 6096
UniProt Q92753
Pathways Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway

Application Details

Product Details anti-RORB Antibody Target Details RORB Handling Images back to top
Application Notes Immunohistochemistry-Paraffin 5 μg/mL
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for ROR beta Antibody (NLS2374)Immunohistochemistry
1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
. Make 4 um sections and place on pre-cleaned and charged microscope slides.
. Heat in a tissue-drying oven for 45 minutes at 60 degrees Celcius.
. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each at RT.
. Rehydrate the tissues by washing the slides in 3 changes of 100 % alcohol for 3 minutes each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 3 minutes each at RT.
. Wash the slides in 1 change of 80 % alcohol for 3 minutes at RT.
. Rinse the slides in gentle running distilled water for 5 minutes at RT.
. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.
0) at 99-100 degrees Celciusfor 20 minutes.
. Remove the slides from the heat and let stand in buffer at RT for 20 minutes.
. Rinse the slides in 1X TBS-T for 1 minute at RT.**Do not allow the tissues to dry at any time during the staining procedure**
. Begin the immunostaining by applying a universal protein block for 20 minutes at RT.
. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase steptavidin for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase chromagen substrate for 30 minutes at RT.
. Rinse the slide in distilled water for 1 minute at RT.**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**
. Dehydrate the tissue by washing the slides in 2 changes of 80 % alcohol for 1 minute each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of 100 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of xylene for 1 minute each at RT.
. Apply cover slip.
Restrictions For Research Use only

Handling

Product Details anti-RORB Antibody Target Details RORB Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Images

Product Details anti-RORB Antibody Target Details RORB Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-RAR-Related Orphan Receptor B (RORB) antibody (ABIN270818) Immunohistochemistry-Paraffin: ROR beta Antibody [NLS2374] - Human Skin, Melanoma. Fo...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-RAR-Related Orphan Receptor B (RORB) antibody (ABIN270818) Immunohistochemistry-Paraffin: ROR beta Antibody [NLS2374] - Analysis of anti-ROR Bet...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-RAR-Related Orphan Receptor B (RORB) antibody (ABIN270818) Immunohistochemistry-Paraffin: ROR beta Antibody - Anti-RORB / ROR Beta antibody IHC...