anti-Rat (Rattus) Splicing Factor 1 antibody for Immunohistochemistry

Recommended Splicing Factor 1 Antibody (supplied by: Log in to see )

Antigen
Splicing Factor 1 (SF1) Antibodies
  • BBP
  • D11S636
  • MBBP
  • ZCCHC25
  • ZFM1
  • ZNF162
  • BB094781
  • CW17R
  • MZFM
  • WBP4
  • Zfp162
  • CG5836
  • Dmel\\CG5836
  • cg5836
  • dSF1
  • p70
  • SF1
  • sf1
  • wu:fc09f06
  • znf162
  • MWD22.25
  • MWD22_25
  • splicing factor 1
  • Splicing factor 1
  • splicing factor-like protein
  • splicing factor 1 S homeolog
  • SF1
  • Sf1
  • sf1
  • AT5G51300
  • sf1.S
Epitope
AA 160-266
18
13
10
10
7
7
7
6
5
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
110
59
32
19
17
16
14
12
11
10
8
7
3
2
1
1
Host
Rabbit
97
13
Clonality
Polyclonal
Conjugate
This Splicing Factor 1 antibody is un-conjugated
2
2
2
2
2
2
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
104
60
54
32
27
7
6
5
3
2
1
Options
Supplier
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Available images

Catalog No. ABIN5692946
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
15.987052 ABIN2776691 IHC WB Rabbit N-Term Log in to see Polyclonal 1
15.987052 ABIN364486 ELISA IHC IHC (p) Rabbit IgG AA 10-25 Log in to see Polyclonal 0
15.987052 ABIN6270202 ELISA IHC WB Rabbit IgG pSer80, pSer82 Log in to see Polyclonal 0
12.987052 ABIN214143 IHC IHC (p) WB Rabbit IgG AA 517-566 Log in to see Polyclonal 0
12.987052 ABIN6570432 IHC WB Rabbit IgG Log in to see Polyclonal 0
12.987052 ABIN2559895 IHC WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN2969210 IHC WB Rabbit IgG Log in to see Polyclonal 0
9.987052 ABIN2777969 IHC WB Rabbit Middle Region Log in to see Polyclonal 1
9.987052 ABIN1934096 IHC IHC (p) WB Rabbit AA 65-114 Log in to see Polyclonal 0
9.987052 ABIN1934093 IHC IHC (p) WB Rabbit AA 215-264 Log in to see Polyclonal 0
7 ABIN203234 IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
4 ABIN6712123 IF IHC WB Rabbit AA 45-98 Log in to see Polyclonal 0
4 ABIN6716554 IHC WB Rabbit Log in to see Polyclonal 0
1 ABIN3198312 IC IF IHC WB Rabbit N-Term, pSer82 Log in to see Polyclonal 0
1 ABIN2628220 ICC IHC IHC (fro) IHC (p) WB Rabbit IgG AA 11-30 Log in to see Polyclonal 0
1 ABIN2881677 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN4218856 IHC IHC (p) WB Rabbit pSer82 Log in to see Polyclonal 0
1 ABIN2912503 ELISA IHC Rabbit IgG Log in to see Polyclonal 0
1 ABIN1092861 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
1 ABIN6240993 IHC WB Rabbit pSer82 Log in to see Polyclonal 0

General

Antigen Splicing Factor 1 (SF1) Antibodies
Epitope AA 160-266
(18), (13), (10), (10), (7), (7), (7), (6), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(110), (59), (32), (19), (17), (16), (14), (12), (11), (10), (8), (7), (3), (2), (1), (1)
Host Rabbit
(97), (13)
Clonality
Polyclonal
Conjugate This Splicing Factor 1 antibody is un-conjugated
(2), (2), (2), (2), (2), (2), (1)
Application ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(104), (60), (54), (32), (27), (7), (6), (5), (3), (2), (1)
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Product Details anti-Splicing Factor 1 Antibody

Target Details Splicing Factor 1 Application Details Handling Images
Brand Picoband™
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for splicing factor 1 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.
Immunogen E. coli-derived human splicing factor 1 recombinant protein (Position: R160-Q266).
Plasmids, Primers & others

Target Details Splicing Factor 1

Product Details anti-Splicing Factor 1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SF1 (SF1 Antibody Abstract)
Background

Synonyms: Splicing factor 1, Mammalian branch point-binding protein, BBP, mBBP, Transcription factor ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, SF1, ZFM1, ZNF162

Tissue Specificity: Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.

Background: Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

UniProt Q15637
Pathways Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location

Application Details

Product Details anti-Splicing Factor 1 Antibody Target Details Splicing Factor 1 Handling Images back to top
Application Notes

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Application Details: Western blot, 0.1-0.5&mu,g/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1&mu,g/mL
Direct ELISA, 0.1-0.5&mu,g/mL

Restrictions For Research Use only

Handling

Product Details anti-Splicing Factor 1 Antibody Target Details Splicing Factor 1 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Buffer Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-Splicing Factor 1 Antibody Target Details Splicing Factor 1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Splicing Factor 1 (SF1) (AA 160-266) antibody (ABIN5692946) Western blot analysis of splicing factor 1 using anti-splicing factor 1 antibody . E...
Immunohistochemistry (IHC) image for anti-Splicing Factor 1 (SF1) (AA 160-266) antibody (ABIN5692946) IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody . splicing fa...
Immunohistochemistry (IHC) image for anti-Splicing Factor 1 (SF1) (AA 160-266) antibody (ABIN5692946) IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody . splicing fa...
Immunohistochemistry (IHC) image for anti-Splicing Factor 1 (SF1) (AA 160-266) antibody (ABIN5692946) IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody . splicing fa...
Immunohistochemistry (IHC) image for anti-Splicing Factor 1 (SF1) (AA 160-266) antibody (ABIN5692946) IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody . splicing fa...
Immunohistochemistry (IHC) image for anti-Splicing Factor 1 (SF1) (AA 160-266) antibody (ABIN5692946) IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody . splicing fa...
Immunohistochemistry (IHC) image for anti-Splicing Factor 1 (SF1) (AA 160-266) antibody (ABIN5692946) IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody . splicing fa...