anti-Rat (Rattus) Splicing Factor 1 antibody for Immunohistochemistry

Recommended Splicing Factor 1 Antibody (supplied by: Log in to see )

Antigen
Splicing Factor 1 (SF1) Antibodies
  • BBP
  • D11S636
  • MBBP
  • ZCCHC25
  • ZFM1
  • ZNF162
  • BB094781
  • CW17R
  • MZFM
  • WBP4
  • Zfp162
  • CG5836
  • Dmel\\CG5836
  • cg5836
  • dSF1
  • p70
  • SF1
  • sf1
  • wu:fc09f06
  • znf162
  • MWD22.25
  • MWD22_25
  • splicing factor 1
  • Splicing factor 1
  • splicing factor-like protein
  • splicing factor 1 S homeolog
  • SF1
  • Sf1
  • sf1
  • AT5G51300
  • sf1.S
Epitope
Middle Region
17
13
10
10
7
7
7
6
5
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
106
56
29
18
16
15
13
11
11
9
8
7
3
2
1
1
Host
Rabbit
93
13
Clonality
Polyclonal
Conjugate
This Splicing Factor 1 antibody is un-conjugated
2
2
2
2
2
2
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
100
61
50
31
27
7
6
5
3
2
1
Options
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Catalog No. ABIN2777969
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.404629 ABIN2776691 IHC WB Rabbit N-Term Log in to see Polyclonal 1
8.404629 ABIN364486 ELISA IHC IHC (p) Rabbit IgG AA 10-25 Log in to see Polyclonal 0
8.404629 ABIN6270202 ELISA IHC WB Rabbit IgG pSer80, pSer82 Log in to see Polyclonal 0
8.404629 ABIN214143 IHC IHC (p) WB Rabbit IgG AA 517-566 Log in to see Polyclonal 0
8.404629 ABIN5692946 ELISA IHC WB Rabbit AA 160-266 Log in to see Polyclonal 0
8.404629 ABIN1934093 IHC IHC (p) WB Rabbit AA 215-264 Log in to see Polyclonal 0
8.404629 ABIN1934096 IHC IHC (p) WB Rabbit AA 65-114 Log in to see Polyclonal 0
8.404629 ABIN5964190 IHC WB Rabbit IgG Log in to see Polyclonal 0
8.404629 ABIN2559895 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN3198312 IC IF IHC WB Rabbit N-Term, pSer82 Log in to see Polyclonal 0
1 ABIN203234 IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN2628220 ICC IHC IHC (fro) IHC (p) WB Rabbit IgG AA 11-30 Log in to see Polyclonal 0
1 ABIN2969210 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2881677 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN4218856 IHC IHC (p) WB Rabbit pSer82 Log in to see Polyclonal 0
1 ABIN2912503 ELISA IHC Rabbit IgG Log in to see Polyclonal 0
1 ABIN1092861 IHC ELISA Rabbit IgG Log in to see Polyclonal 0

General

Antigen Splicing Factor 1 (SF1) Antibodies
Epitope Middle Region
(17), (13), (10), (10), (7), (7), (7), (6), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(106), (56), (29), (18), (16), (15), (13), (11), (11), (9), (8), (7), (3), (2), (1), (1)
Host Rabbit
(93), (13)
Clonality
Polyclonal
Conjugate This Splicing Factor 1 antibody is un-conjugated
(2), (2), (2), (2), (2), (2), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(100), (61), (50), (31), (27), (7), (6), (5), (3), (2), (1)
Pubmed 1 reference available
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Product Details anti-Splicing Factor 1 Antibody

Target Details Splicing Factor 1 Application Details Handling References for anti-Splicing Factor 1 antibody (ABIN2777969) Images
Blocking Peptide Blocking peptide for this product available: ABIN983611
Sequence VKKAVEQIRN ILKQGIETPE DQNDLRKMQL RELARLNGTL REDDNRILRP
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%
Characteristics This is a rabbit polyclonal antibody against SF1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human SF1
Plasmids, Primers & others

Target Details Splicing Factor 1

Product Details anti-Splicing Factor 1 Antibody Application Details Handling References for anti-Splicing Factor 1 antibody (ABIN2777969) Images back to top
Antigen
Alternative Name SF1 (SF1 Antibody Abstract)
Background SF1 contains 1 CCHC-type zinc finger and 1 KH domain. SF1 is Necessary for the ATP-dependent first step of spliceosome assembly. It binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. SF1 may act as transcription repressor.
Alias Symbols: D11S636, ZFM1, ZNF162, BBP, MBBP
Protein Interaction Partner: KRTAP26-1, TRIM69, FAM168A, CIAO1, MKRN3, PSMA3, FUS, UBC, NEDD8, SUZ12, EED, RPL24, PLOD2, OSBP, NARS, GFPT1, GART, EIF4B, APBB1, SUPT5H, TROVE2, ITCH, PRPF40A, SAMHD1, XPO7, WBP4, FAF1, TCERG1, HSPH1, FARSB, GAS7, RANBP3, HIRIP3, HIST1H4A, XRCC5, U2AF2,
Protein Size: 639
Molecular Weight 68 kDa
Gene ID 7536
NCBI Accession NM_004630, NP_004621
UniProt Q15637
Pathways Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location

Application Details

Product Details anti-Splicing Factor 1 Antibody Target Details Splicing Factor 1 Handling References for anti-Splicing Factor 1 antibody (ABIN2777969) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 639 AA

Restrictions For Research Use only

Handling

Product Details anti-Splicing Factor 1 Antibody Target Details Splicing Factor 1 Application Details References for anti-Splicing Factor 1 antibody (ABIN2777969) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Splicing Factor 1 antibody (ABIN2777969)

Product Details anti-Splicing Factor 1 Antibody Target Details Splicing Factor 1 Application Details Handling Images back to top
Background publications

Rino, Desterro, Pacheco, Gadella, Carmo-Fonseca: "Splicing factors SF1 and U2AF associate in extraspliceosomal complexes." in: Molecular and cellular biology, Vol. 28, Issue 9, pp. 3045-57, 2008

Images

Product Details anti-Splicing Factor 1 Antibody Target Details Splicing Factor 1 Application Details Handling References for anti-Splicing Factor 1 antibody (ABIN2777969) back to top
Supplier Images
Western Blotting (WB) image for anti-Splicing Factor 1 (SF1) (Middle Region) antibody (ABIN2777969) WB Suggested Anti-SF1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:12500 Positi...
Immunohistochemistry (IHC) image for anti-Splicing Factor 1 (SF1) (Middle Region) antibody (ABIN2777969) Rabbit Anti-SF1 Antibody Formalin Fixed Paraffin Embedded Tissue: Human heart Tissu...