anti-Mouse (Murine) APAF1 antibody for Immunoprecipitation

Recommended APAF1 Antibody (supplied by: Log in to see )

Antigen
Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
  • APAF1
  • ARK
  • Apaf-1
  • Apaf1
  • CG6829
  • D-Apaf-1
  • DARK
  • Dapaf-1
  • Dapaf-1/HAC-1
  • Dark
  • Dark/Apaf-I
  • Dark/Dapaf-1/HAC1
  • Dark/Hac-1/dApaf-1
  • Dark/Hac-1/dApaf1
  • Dmel\\CG6829
  • Hac-1
  • Hac-1/Dark
  • Hac1
  • T1
  • anon-53Fa
  • apaf-1
  • apaf1
  • arc
  • ark
  • dAPAF-1
  • dApaf-1
  • dApaf-1/DARK/HAC-1
  • dApaf1
  • dArk
  • dapaf
  • dapaf-1
  • dapaf-1L
  • dapaf-1S
  • dark
  • dark/dapaf-1/hac-1
  • dark/hac-1/dapaf-1
  • hac-1
  • hac1
  • l(2)SH0173
  • APAF-1
  • CED4
  • 6230400I06Rik
  • Apaf1l
  • fog
  • mKIAA0413
  • zgc:136543
  • Death-associated APAF1-related killer
  • apoptotic peptidase activating factor 1 L homeolog
  • apoptotic peptidase activating factor 1
  • Dark
  • apaf1.L
  • apaf1
  • APAF1
  • Apaf1
Epitope
AA 1-97, N-Term
39
35
15
13
13
9
5
5
5
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
199
120
103
7
4
3
3
3
2
1
Host
Rat
171
25
15
2
Clonality
Monoclonal
Conjugate
This APAF1 antibody is un-conjugated
4
4
4
2
2
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
162
91
71
55
41
27
22
18
10
7
4
4
3
Options
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Catalog No. ABIN2745509
$ 481.56
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.572235 ABIN2745510 ICC IP ELISA WB Rat IgG2a AA 1-97, N-Term Log in to see 18H2 0
12.572235 ABIN1690175 IP Magnetic Particles Rabbit Log in to see Polyclonal 0
12.572235 ABIN1690523 IP Sepharose Rabbit Log in to see Polyclonal 0
10 ABIN957135 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 0
7 ABIN2908263 IF/ICC IHC IP WB Rabbit AA 872-1135 Log in to see Polyclonal 0
1 ABIN204433 ICC IP ELISA WB Rat IgG2a AA 1-97 Log in to see 0
1 ABIN204427 ICC IP WB Rat IgG2a AA 1-97 Log in to see 0
1 ABIN5690622 ELISA ICC IP WB Rat IgG2a, kappa AA 1-97 Log in to see 13F11 0
1 ABIN5690623 ELISA ICC IP WB Rat IgG2a, kappa AA 1-97 Log in to see 18H2 0
1 ABIN1495161 IP Sepharose Rabbit N-Term Log in to see Polyclonal 0
1 ABIN1495160 IP Magnetic Particles Rabbit N-Term Log in to see Polyclonal 0
1 ABIN2616746 ELISA ICC IP WB Rat IgG2a, kappa AA 1-97 Log in to see 13F11 0
1 ABIN2616747 ELISA ICC IP WB Rat IgG2a, kappa AA 1-97 Log in to see 18H2 0
1 ABIN326854 IP IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2201993 IC IP WB Rat IgG2a AA 1-97 Log in to see 6D120 0
1 ABIN2201994 IC IP ELISA WB Rat IgG2a AA 1-97 Log in to see 6D121 0
1 ABIN6029004 IF/ICC IHC IP WB Rabbit Log in to see Polyclonal 0

General

Antigen Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
Epitope AA 1-97, N-Term
(39), (35), (15), (13), (13), (9), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(199), (120), (103), (7), (4), (3), (3), (3), (2), (1)
Host Rat
(171), (25), (15), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This APAF1 antibody is un-conjugated
(4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(162), (91), (71), (55), (41), (27), (22), (18), (10), (7), (4), (4), (3)
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Product Details anti-APAF1 Antibody

Target Details APAF1 Application Details Handling Images
Specificity Recognizes the CARD domain of mouse and rat Apaf-1.
Cross-Reactivity Mouse (Murine), Rat (Rattus)
No Cross-Reactivity Human
Cross-Reactivity (Details) Does not cross-react with human Apaf-1.
Purity >95 % (SDS-PAGE)
Endotoxin Level <0.1EU/μg purified protein (LAL test, Lonza).
Immunogen Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain.
Clone 13F11
Isotype IgG2a
Plasmids, Primers & others

Target Details APAF1

Product Details anti-APAF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Apaf-1 (APAF1 Antibody Abstract)
Background Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
UniProt Q9EPV5
Pathways p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-APAF1 Antibody Target Details APAF1 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer In PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.
Expiry Date 12 months